109 articles for “DNA analysis”

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InvestigationOpen accessIn the abstractGenetic Structure of Populations of Kazakh Whiteheaded and Hereford Cattles on the Basis of Microsatellite DNAAdas Tileubekovna Tyngoziyeva, Talgat Nikolaevich Karymsakov, Serik Doldashevich NurbaevThis work is aimed at determination of genetic dissimilarities between two allied cattle breeds: Kazakh Whiteheaded and Hereford. Hair follicles were analyzed, from which DNA was extracted with subsequent amplification. Identification of amplification products was performed using an ABI Prism 310 genetic analyzer. Allele 147 was detected in Kazakh Whiteheaded cattle in locus BM 2113, there was no such allele … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 6 Dec 2017 · 7,427 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Investigation of Organelle Genomes of Extra Nuclear Sunflower Mutants with Variegated PhenotypeIrina Dremuk, Nikolay Kozel, Mariya Logacheva et al.The comparative analysis of complete chloroplast and mitochondrial DNA sequences of the original inbred line 3629 and three extra nuclear mutant lines variegated-1, variegated-15, variegated-17, obtained by the N-nitroso-N-methylurea induced mutagenesis, was done. The studied mutant lines were presented two different phenotypes with diverse chlorophyll content: Pale/green mutant line variegated-17 with 1.8% relative (to 3629 line) chlorophyll content in mutant … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 24 Oct 2017 · 7,633 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPhylogenetic of ERIC-DNA Fingerprinting and New Sequencing of Aeromonas Species and V. Cholerae DNAHawraa Natiq Kabroot AL-Fatlawy, Hawraa Abdalameer Aldahhan, Ali Hmood AlsaadiThe current study included 44 isolate of A. hydrophila, A. sobria and V. cholerae and other bacteria isolated from stool samples and environmental samples (Kufa river water and hospital environmental samples). ERIC DNA Fingerprinting with ERIC primers pairs generated distinct amplification bands ranging in size from (87 bp to 8000 kb). The 44 isolates produced 93 different patterns by ERIC … …American Journal of Applied Sciences, Vol. 14, Issue 10 · 5 May 2017 · 7,801 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPhylogeny and Molecular Study of Some Entomopathogenic Rhizobacteria Isolated from Two Regions in AlgeriaOulebsir-Mohandkaci Hakima, Benzina Farida, Khemili-Talbi Souad et al.The use of bacteria in the control of insect pests is a form of biological control whose practice is still not widespread. It is in this context that the present work falls. It concerns the isolation, characterization and identification of local bacterial strains for the purpose of their use in the control of certain pests. Indeed, 20 bacteria were isolated … …Current Research in Bioinformatics, Vol. 8, Issue 1 · 2 Mar 2020 · 7,231 accessesView PDF Cite

Research ArticleOpen accessIn the abstractValidation of SSR markers associated with Submergence Tolerance in Rice (Oryza sativa L.)Kirill V. Azarin, Alexander V. Usatov, Andrey V. Alabushev et al.The present work is devoted to the validation of SSR markers associated with submergence tolerance (Sub1A) QTLs in rice. It was shown that only microsatellite markers RM 7481 differentiates donor lines and recipient variety. Based upon the results of DNA analysis with the marker of the RM 7481 of the F2-plants, the 14 homozygotes by the locus Sub1A, 40 samples … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 4 · 19 Dec 2016 · 7,634 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCpG Oligodeoxynucleotides Debate the Damaging Action of Cisplatin on Immune Cell DNA: A PCR Array Application on Repair Genes ExpressionNaglaa Fathy Alhusseini, Amal Abo El-Fadl, Shabaan Hemada et al.Synthetic Oligodeoxynucleotides (ODN) expressing CpG motifs (CpG-ODN) mimic the immunostimulatory activity of bacterial DNA and are commonly used to activate Toll Like Receptor 9 (TLR9) for therapeutic applications. CpG-ODN can counteract the activity of DNA-damaging chemotherapy and radiation therapy on immune cell and enhance immunity in preclinical mouse models. We hypothesized that these actions of CpG-ODN on immune cells are … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 3 · 27 Jul 2016 · 7,625 accessesView PDF Cite

Research ArticleOpen accessIn the abstractVariability of Chloroplast DNA of Extranuclear Sunflower MutantsMarkin Nicolay, Aieksandr Usatov, Maria Logacheva et al.A comparative analysis of full-genome sequences of chloroplast DNA (cpDNA) of the original inbred line 3629 and three extranuclear mutants, which were obtained by the method of mutagenesis induced with N-nitroso-N-methylurea (NMU) and characterized by different level of chlorophyll insufficiency (en:chlorina-7-yellow-green leaves; chlorophyll content (a + b)-67.8% with respect to the line 3629, variegated-10-leaves with white zones; chlorophyll content (a … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Mar 2016 · 7,269 accessesView PDF Cite

Research ArticleOpen accessIn the abstractComparative Genomics of Domesticated and Wild Sunflower: Complete Chloroplast and Mitochondrial GenomesM. S. Makarenko, A. V. Usatov, N. V. Markin et al.The entire chloroplast and mitochondrial genomes of domesticated and wild type sunflower were sequenced. The comparative analysis of chloroplast genomes revealed 43 variant sites, including 21 polymorphic SSR loci and 22 SNPs. About 14 variant sites were found by collation of mitochondrial DNA (mtDNA), among them 4 SSRs, 8 SNPs and 2 deletions. About 9 SNPs were located in coding … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 29 Feb 2016 · 7,775 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBIS-CATTLE: A Web Server for Breed Identification using Microsatellite DNA MarkersSarika Jaiswal, Sandeep Kumar Dhanda, M. A. Iquebal et al.Domestic cow, Bos taurus is one of the important species selected by humans for various traits, viz. milk yield, meat quality, draft ability, resistance to disease and pests and social and religious reasons. Since cattle domestication from Neolithic (8,000-10,000 years ago) today the population has reached 1.5 billion and further it’s likely to be 2.6 billion by 2050. High magnitude … …Current Research in Bioinformatics, Vol. 5, Issue 1 · 23 Jan 2016 · 9,310 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCytogenetic Analysis among Train Depot Workers Exposed to Total Volatile Organic CompoundsNormah Awang, Siti Adillah Isah, Asmah HamidOccupational exposure to toxic chemicals such as Total Volatile Organic Compounds (TVOC) from diesel engine exhaust implicates genotoxic risk. The objective of this study was to determine the DNA damage among KL Sentral depot workers exposed to TVOC by means of cytogenetic analysis. Data were obtained partially from disseminated questionnaires and buccal cells samples were collected from a total of … …OnLine Journal of Biological Sciences, Vol. 15, Issue 2 · 12 Jun 2015 · 7,443 accessesView PDF Cite