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86 articles for “Cell Culture”
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Research ArticleOpen accessIn the abstractCELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOSNicole Mika, Katharina Cierpka, Michaela C. Lange et al.As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Feb 2014 · 8,157 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Study on Bioelectricity Production by the Synergistic Action of Bacillus tequilensis DMR-5 and Pseudomonas aeruginosa DMR-3 Isolated from Rumen FluidJothinathan Deepika, Sundaram Meignanalakshmi, Wilson Richard ThilagarajThe present study focuses on the bioelectricity production from pure culture of Bacillus tequilensis DMR-5 and Pseudomonas aeruginosa DMR-3. Both the cultures were isolated from the anodic biofilm of pre-run MFC’s with rumen fluid as anodic substance. They were checked for the power production for 10 days both as pure isolates and co-culture. Bacillus tequilensis and Pseudomonas aeruginosa produced 250 … …American Journal of Environmental Sciences, Vol. 9, Issue 5 · 29 Oct 2013 · 6,948 accessesView PDFCite
Research ArticleOpen accessIn the abstractFREE ENERGY MEASUREMENT DISTINGUISHES NORMAL FROM CANCER CELL, OFFERING A NEW PERSPECTIVE FOR CURING CANCERKambiz Afrasiabi, Jonathan Melekh-ShalomThe interplay of the second law of thermodynamics with the normal state and the methodology needed for the measurement of free energy of normal and malignant cells and its practical implications has not been clearly addressed in current literature. The second law of thermodynamics is one of the most fundamental laws governing the known universe at all levels. A normal … …OnLine Journal of Biological Sciences, Vol. 13, Issue 4 · 12 Oct 2013 · 7,224 accessesView PDFCite
Research ArticleOpen accessIn the abstractCancerous Immunoglobulins and CA215: Implications in Cancer ImmunologyGregory LeeImmunoglobulins are typically expressed by B cells in our normal immune system. However, certain normal human tissues, such as hyperplastic epithelial cells, cells of the immunologically privileged sites and the majority of cancer cells, have also been found to be sites of immunoglobulin production. Current research is lacking in regards to the differential immunoglobulin expression, the underling mechanisms of action … …American Journal of Immunology, Vol. 8, Issue 4 · 18 Sep 2012 · 8,942 accessesView PDFCite
Research ArticleOpen accessIn the abstractKEY PARAMETERS OF MEASLES VIRUS PRODUCTION FOR ONCOLYTIC VIROTHERAPYK. Weiss, D. Salzig, M. D. Mühlebach et al.Attenuated measles virus has revealed selective tumor cell killing and is currently tested in clinical trials for the therapy of cancer patients. The amount of infectious particles per dose needed for oncolytic therapy can be more than a million times higher compared to vaccination. This requires highly effective production processes which guarantee the measles virus quantities needed for its use … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 9,785 accessesView PDFCite
Research ArticleOpen accessIn the abstractFoxp3-Mediated Immunity of Human Pancreatic Cancer Cell Line PANC-1Renxi Wang, Gencheng Han, Jianan Wang et al.Problem statement: More and more evidences have shown that human cancer cells can express Foxp3, the regulatory T cell-associated transcription factor. The role of Foxp3 in human cancer cells is still unclear. We detected Foxp3 expression in human pancreatic cancer cell line PANC-1. SiRNA assays showed that Foxp3 expression suppressed the expression of TGF-β and IL-10 which could induce immune … …American Journal of Immunology, Vol. 5, Issue 4 · 11 Jan 2010 · 7,363 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction and Roles of Glial Tissue Inhibitor of Metalloproteinases-1 in Human Immunodeficiency Virus-1-Associated Dementia Neuroinflammation: A ReviewC. Chao, A. GhorpadeProblem statement: Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) and its cognate targets, the Matrix Metalloproteinases (MMPs), were differentially expressed in human brain samples with or without HIV-1 infection or HIV-1 Encephalitis (HIVE). Approach: A through literature review demonstrated that cell culture models of Central Nervous System (CNS) cell types had been used to illustrate the intricate temporal patterns of TIMP-1/MMP expression, … …American Journal of Infectious Diseases, Vol. 5, Issue 4 · 31 Dec 2009 · 6,953 accessesView PDFCite
Research ArticleOpen accessIn the abstractKinetics of Xylanase Fermentation by Recombinant Escherichia coli DH5α in Shake Flask CultureFarliahati Mohd Rusli, Mohd Shamzi Mohamed, Rosfarizan Mohamad et al.Problem statement: Interest in xylanase enzyme application has markedly increased in pulp and paper processing industries. The switch to xylanase-producing recombinant Escherichia coli DH5α pTP510 is seen here as an economic alternative towards higher productivity and easier downstream purification. Modeling of E. coli DH5α growth and enzyme secretion is thus desired for future optimization in fermentation process. Approach: Kinetics of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,480 accessesView PDFCite
Research ArticleOpen accessIn the abstractMembrane Binding Activity with Virus in White Spot Syndrome Virus-Infected Fenneropenaeus chinensisLiu Qing-hui, Huang Jie, Chen Ting, Yang BingProblem statement: White Spot Syndrome Virus (WSSV) is one of the most disastrous pathogens in shrimp culture, having caused high mortality in many cultured shrimp species. It is well known that the most important step of viral infection is cell attachment. Approach:Therefore determining the binding activity of shrimp cells to WSSV is an important to evaluate anti-infection ability and understanding … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 2 · 30 Jun 2009 · 7,496 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Use of Artificial Neural Networks in Analysis Cationic Trypsinogen Gene and Hepatitis B Surface AntigenLiu Qicai, Zeng Kai, Zhuang Zehao et al.Problem statement: More and more severe hepatitis cases reported in China are infected with HBV and the immune response of HBV will be reduced if mutations occur in the TCR, therefore it is very important to investigate the relation of T-cellular function and clinical effect by studying the function of T-cell receptor. Approach: Artificial Neural Networks (ANN) was applied to … …American Journal of Immunology, Vol. 5, Issue 2 · 30 Apr 2009 · 7,564 accessesView PDFCite