34 results for “sucrose”

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Research ArticleOpen accessIn the abstractHeterologous Expression and Characterization of Thermostable Levansucrase (BsSacB) from Bacillus subtilis BB03Viniti Vaidya, Gajjeraman Prabu, D. Theertha PrasadThermostable levansucrase encoding gene (sacB) with its promoter was isolated from Bacillus subtilis BB03. BsSacB is composed of 1419 bp, encoding 473 amino acid residues and with promoter regions TTGCAA (-35) and TAGAAT (-10). The amino acid sequence analysis of BsSacB showed conserved motifs of microbial fructosyltransferase belonging to Glycoside Hydrolase family 68 (GH68). Comparative analysis of the protein structure … …OnLine Journal of Biological Sciences, Vol. 15, Issue 1 · 25 Mar 2015 · 7,371 accessesView PDF Cite

EditorialOpen accessIn the abstractRe-Paving the Road Built by Chemistry: A Challenge to Biochemistry and BiotechnologyAlberto A. IglesiasBiochemistry’s birth can be placed at the end of the nineteenth century, when Eduard Buchner made a clever interpretation of experimental observations on the generation of gas bubbles after addition (the original purpose was preservation) of sucrose to a yeast crude extract (Lagerkvist, 2005). Intelligently, Buchner concluded that the disaccharide had been fermented by broken cells’ suspension to produce CO2, … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 1 · 26 Jan 2015 · 7,461 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInulinase Production by Penicillium citrinum ESS in Submerged and Solid-State CulturesCynthia Lafuente-Castaneda, Gabriela Martinez, Juan C. Contreras-Esquivel et al.Problem statement: This work reports the Penicillium citrinum ESS strain as a new source of inulinase (2,1-β-D fructanhydrolase, E.C. 3.2.1.7). Approach: The enzyme was produced in both, Solid-State (SSC) and Submerged Culture (SmC) using a basal medium added either with inulin or sucrose (5 gL-1). Results: The culture grew faster in SmC than in SSC with both carbon sources; however, … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 2 · 13 Mar 2012 · 8,388 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEffect of Equilibration Temperature on In vitro Viability and Subsequent Embryo Development of Vitrified-Warmed Immature Bovine OocytesHajarian Hadi, H. Wahid, O. Abas Mazni et al.Problem statement: Vitrification is replacing conventional slow freezing to cryopreserve gametes and embryos especially for in vitro production of embryo in domestic animal species. However, the results are still not satisfactory. The aim of this experiment was to study the effect of different equilibration temperatures on in vitro viability of immature bovine oocytes after vitrification. Approach: Oocytes were obtained from … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 2 · 1 Sep 2010 · 16,116 accessesView PDF Cite

Research ArticleOpen accessIn the abstractAn in vitro Assessment on the Efficacy of Clay-Based Formulated Cells of Pseudomonas Isolate UTAR EPA2 for Petrol DegradationA. S.Y. Ting, M. T. Fang, C. S. TeeProblem statement: Application of free-cell forms is usually impractical to achieve satisfactory bioremediative effect because the microbes are encumbered by the biotic and abiotic stresses from the environment. Approach: In this study, a hydrocarbon-degrading bacterium (Pseudomonas isolate UTAR EPA2) was formulated with various combinations of formulative materials, comprising of clay-based carrier materials such as Bentonite (B) and Kaolin (K), enrichment … …American Journal of Applied Sciences, Vol. 7, Issue 2 · 28 Aug 2010 · 7,650 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPrevalence of Stray Dogs with Intestinal Protozoan ParasitesMohammad MirzaeiProblem statement: Intestinal protozoan parasites are important enteropathogens in dogs. Moreover, several canine intestinal protozoan parasites are zoonotic and are considered important to public health. This study investigates the level of intestinal protozoan parasites in stray dogs, in Kerman city, Iran. Approach: Determination of the prevalence of infections was based on faecal examination. Stool samples (n = 98) collected from … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 2 · 30 Jun 2010 · 8,513 accessesView PDF Cite

Research ArticleOpen accessIn the abstractSugar Beet Performance Affected by Uniformity of N FertigationH. Nouri, M. S.M. Amin, S. J. Razavi et al.In common with the majority of crop species, in sugar beet nutrition nitrogen is an important element due to its role in sucrose utilization and plant growth. Application of precision agriculture, in order to apply the right amount of input at the right time in the right area, is appropriate to decrease chemical use and increase plant growth. Problem statement: … …American Journal of Applied Sciences, Vol. 7, Issue 3 · 31 Mar 2010 · 7,931 accessesView PDF Cite

Research ArticleOpen accessIn the abstractChilling Injury in Mamey Sapote Fruit (Pouteria sapota): Biochemical and Physiological ResponsesGuillermo O. Pérez-Tello, Miguel Ángel Martínez-Téllez, Irasema Vargas-Arispuro, Gustavo A. González-AguilarProblem statement: Cold storage is needed in order to delay senescence and achieve a longer commercial life of tropical fruits like mamey sapote (Pouteria sapota), therefore, the physiological and biochemical responses of this fruit to cold storage were evaluated. Approach: Samples from different storage temperature (20, 10 and 2°C) were taken at five-day intervals and Chilling Injury Index (CII), decay … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 2 · 30 Jun 2009 · 9,162 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDevelopment of Vegetable Based Milk from Decorticated Sesame (Sesamum Indicum)Jihad M. Quasem, Ayman Suliman Mazahreh, Khaled Abu-AlruzProblem statement: Decorticated sesame seed can supply us with nutritious, functional and healthy meals with relatively low cost. These properties make sesame a valuable source of protein and other nutrients to be utilized in imitated dairy products, which could be used for infant and adults with lactose intolerance as well as for vegetarian or others who like to eat dairy … …American Journal of Applied Sciences, Vol. 6, Issue 5 · 31 May 2009 · 11,299 accessesView PDF Cite