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30 articles for “immobilized”
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Research ArticleOpen accessIn the abstractTailor-Made Enzyme Carriers: Preparation and Use of Adsorbents Specifically Designed to Immobilize Allosteric Enzymes in Activated ConformationZahra SalemiProblem statement: The enzyme immobilization has experienced substantial growth in the recent past and an ever increasing amount of study has been reported on various aspects of immobilized enzymes. In most of these investigations, catalytic activities are found to be diminished as compared to the enzyme free in solution. Approach: Hydrophobic adsorbents were prepared containing L-leucine or citric acid, two … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 7,558 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmobilization of Lipase from Candida rugosa on Chitosan Beads for Transesterification ReactionM. Nasratun, H. A. Said, A. Noraziah, A.N. Abd AllaProblem statement: Further study is recommended to improve the immobilization technique and the immobilized lipase’s performance as catalysis in transesterification reaction. Approach: To investigate the ability of immobilized lipase on chitosan beads to catalyze the transesterification of cooking oil to an ester. The porous bead of chitosan was used for immobilization of lipase from Candida rugosa by physical adsorption. Parameters … …American Journal of Applied Sciences, Vol. 6, Issue 9 · 30 Sep 2009 · 7,605 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization and Wash Performance Analysis of Microbial Extracellular Enzymes from East Calcutta Wetland in IndiaRamesh Malathu, Sanhita Chowdhury, Madhusmita Mishra et al.Extracellular protease from a novel bacterial isolate showing maximum similarity of 98.22% with Microbacterium luteolum was obtained from East Calcutta Wetland, India. It showed compatibility with commercial detergents. The enzyme retains more than 60% of its activity between 6.0 to 10.5 pH. The maximum activity is at pH 7.5 with 71% activity at pH 10.0 and 10.5. The protease retained … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 8,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmobilization of Lipase from Candida Rugosa on Palm-Based Polyurethane Foam as a Support MaterialRoila Awang, Mohd Rafaei Ghazuli, Mahiran BasriLipase from Candida rugosa was immobilized onto palm-based polyurethane foam (PU), which the polymer was pre-soaked in co-immobilized agent. The activities of PU-immobilized lipase were tested by the esterification reaction of oleic acid and oleyl alcohol in hexane. The PU-immobilized lipase was then characterized in term of its thermal, operational and storage stability. The optimum temperature for native and PU-immobilized … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 7,478 accessesView PDFCite
Research ArticleOpen accessIn the abstractRemoval and Recovery of Uranium using Microorganisms Isolated from North American Uranium DepositsTakehiko TsurutaSome attempts were made to remove and recover uranium that may be present in nuclear fuel effluents and mine tailings using microorganisms isolated from North American uranium deposits. To establish which microorganisms accumulate the most uranium, hundreds strains of microorganisms were screened. Of these strains of microorganisms tested, extremely high uranium accumulating ability was found in some bacteria isolated from … …American Journal of Environmental Sciences, Vol. 3, Issue 2 · 30 Jun 2007 · 7,285 accessesView PDFCite
Research ArticleOpen accessIn the abstractNumerical Study of the Moving Boundary Problem During Melting Process in a Rectangular Cavity Heated from BelowY. Jellouli, R. Chouikh, A. Guizani, A. BelghithWe present an analysis of the melting process in Phase Change Materials (PCM) in a horizontal cavity heated from below, driven by the coupling of transient heat conduction in the solid phase and steady state laminar natural convection in the liquid phase with one of the boundaries of the solution domain that moves. We state the problem in the Cartesian … …American Journal of Applied Sciences, Vol. 4, Issue 4 · 30 Apr 2007 · 7,391 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Development of A New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part I: Design of the Detection System and ApplicationsEric Carnes, Ebtisam WilkinsA portable, flow-through amperometric immunoassay system utilizing a newly designed immunosensor has been developed for the detection of bacteria. A sandwich scheme of immunoassay involving two stages of immunoreaction was employed using Escherichia coli as a model. E. coli antibodies were immobilized on Toray carbon paper to create a disposable immunofiltration membrane. Detection of the bacteria was achieved through the … …American Journal of Applied Sciences, Vol. 2, Issue 3 · 31 Mar 2006 · 6,363 accessesView PDFCite
Research ArticleOpen accessIn the abstractMicrobial Population In Decompsing Legume Litter Of Differing QualityM. B. Oyun, F. C. Akharayi, F. C. AdetuyiThe influence of litter quality on microbial population during decomposition of fresh pruning of Acacia auriculiformis; Gliricidia sepium and Acacia mixed with Gliricidia (50 : 50) is reported. Acacia (soluble C, 46.6%; N, 3.9%; Phenolic, 2.3%) was rated as low quality litter while Gliricidia (soluble C, 45.2%; N, 4.8%; Phenolic, 2.3%) was rated as high quality litter. Acacia mixed with … …American Journal of Agricultural and Biological Sciences, Vol. 1, Issue 1 · 31 Mar 2006 · 7,653 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiosensor Based on Xanthine Oxidase for Monitoring Hypoxanthine in Fish MeatHelena S. Nakatani, Luciane V. dos Santos, Cristiano P. Pelegrine et al.An amperometric enzyme electrode for the detection of hypoxanthine in fish meat is described. The hypoxanthine sensor was prepared from xanthine oxidase immobilized by covalent binding with glutaraldehyde and bovine serum albumin on a nafion-coated platinum disc electrode. The responses for hypoxanthine were obtained in 0.1 mol L-1 phosphate buffer (pH 7.0), at potential of 600mV vs Ag/AgCl. This biosensor … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 8,597 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Development of a New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part II: Optimization of the System for Escherichia coliEric Carnes, Ebtisam WilkinsThe optimization of a flow-through immunoassay system was conducted to achieve rapid detection of low concentrations of E. coli bacteria. The system includes a new immunosensor that uses newly developed, disposable immunofiltration membranes to yield a system that is sensitive as well as simple. Comparison of the performance of this system with conventional immunoassays demonstrated a significantly shorter time for … …American Journal of Applied Sciences, Vol. 2, Issue 3 · 31 Mar 2005 · 6,780 accessesView PDFCite