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Research ArticleOpen accessIn the abstractBinding Interaction between Bovine Serum Albumin and Chicoric Acid, a Food Functional ComponentHaifang Xiao, Quancai Sun, Xuebo Liu, Yuanda SongFuorescence, FTIR and UV-Vis absorption spectroscopy were used to explore the binding between chicoric acid and Bovine Serum Albumin (BSA). Binding characteristics at various levels of temperature have been calculated. The results indicated that chicoric acid statically quenched the intrinsic fluorescence of BSA. The binding constants (Ka) were 4.14×105 L mol-1 at 273K and 4.29×106 L mol-1 at 298 k. … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 3 · 19 Sep 2016 · 7,552 accessesView PDFCite
EditorialOpen accessIn the abstractNanotechnology: Engineering Materials for a Better 21st CenturyMeng Qiao, Xiyun Liu, Lei ZhangToday, we are living in the midst of a technological revolution, where nanotechnology is demonstrating a greater commitment to make people’s everyday lives better. The aim of nanotechnology is to create new materials with desired properties by engineering their nanoscale structures and components. One of the most important impacts of nanotechnology is in medical diagnosis and treatment. Cardiovascular diseases such … …Current Research in Nanotechnology, Vol. 5, Issue 1 · 26 Nov 2014 · 8,737 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Pseudozyma hubeiensis Y10BS025 as a Potent Producer of Glycolipid Biosurfactant Mannosylerythritol lipidsMartha Sari, Atit Kanti, I. Made Artika, Wien KusharyotoMannosylerythritol Lipids (MEL’s) are glycolipid biosurfactants that contain 4-O-β-D-mannopyranosyl-meso-erythritol as a hydrophilic moiety and fatty acids as a hydrophobic moiety. MEL’s are abundantly produced by several kinds of microorganism and are one of the most promising biosurfactants currently known. The search for a novel endogenous producer of MEL’s was undertaken based on the available collection of the yeast strains from … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 8,095 accessesView PDFCite
Research ArticleOpen accessIn the abstractSoluble CD59-Antigen Levels are Increased in Renal Disease PlasmaOluseyi Adewale VanderpuyeCluster of Differentiation antigen 59 (CD59) is a glycosyl phosphatidylinositol-linked protein that protects cells from damage by lytic terminal complexes of complement. Soluble forms of CD59 in serum have been little investigated in disease states. In the present study, by using a sandwich ELISA, reactivities with antibodies to CD59 were compared for sera and plasma from normal individuals and those … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 6,944 accessesView PDFCite
Research ArticleOpen accessIn the abstractAroclor 1254 Toxicity to Mice Liver Membrane AtpasesShweta Pathak, Rahul KunduPresent communication reports the sub-acute dose and exposure duration dependent effects of Aroclor 1254 on total, Na+, K+, Ca++ and Mg++-ATPases of the mouse liver cells. The study tests two hypotheses, (a) whether the sublethal dose or the exposure duration dependent in vivo exposure of hydrophobic PCB (Aroclor 1254) affects the hydrophilic membrane-bound ion dependent ATPases of the liver cells … …American Journal of Pharmacology and Toxicology, Vol. 8, Issue 2 · 13 Jul 2013 · 6,791 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,854 accessesView PDFCite
Research ArticleOpen accessIn the abstractDIFFERENT APPROACHES TO CRYSTALLIZATION OF MEMBRANE PROTEINSPrakash G. Doiphode, Vivek Pratap SinghCrystallography is more like an art than science. Crystallizing membrane proteins are a big challenge; membrane proteins are present in the cell membrane and serve as cell support. The most important feature of membrane protein is that it contains both hydrophobic and hydrophilic regions on its surface. They are generally much more difficult to study than soluble proteins. The problem … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 18 Dec 2012 · 7,288 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment of Curcumin Based Ophthalmic FormulationD. Anjana, K. Anitha Nair, N. Somashekara et al.Problem statement: Ophthalmic drug delivery system has been a major challenge due to the distinctive and unique anatomy of the eye. The use of phytochemicals for the application in ocular disease has not been investigated much, due to poor pharmacokinetics of phytochemical and hence poor therapeutic efficacy. Approach: Curcumin is one such phytochemical known for its medicinal properties, but its … …American Journal of Infectious Diseases, Vol. 8, Issue 1 · 24 Feb 2012 · 15,401 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction, Purification and Characterization of Fish Chymotrypsin: A ReviewLiang Zhou, Suzanne M. Budge, Abdel E. Ghaly et al.Problem statement: Solid fish waste is generated from the unwanted parts of fish including heads, tails, fins, frames, offal (guts, kidney and liver) and skin. It accounts for up to 80% of material from production of surimi, 66% from production of fillet and 27% from production of headed and gutted fish. Currently, fish wastes are disposed off in land-based waste … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 22 Sep 2011 · 10,585 accessesView PDFCite