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41 results for “enzyme yield”
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Research ArticleOpen accessIn the abstractEnzymatic Synthesis of Palm Alkyl Ester Using Dialkyl Carbonate as an Alkyl DonorsRoila Awang, Choo Yuen MayProblem statement: Though efficient in terms of reaction yield and time, the chemical approach to synthesizing alkyl ester has drawback such as difficulties in the recovery of glycerol and the need for removal of salt residue. On the other hand, biocatalyst allow for synthesis of specific alkyl esters and easy recovery of glycerol. However, the solvent-free alcoholysis, does not give … …American Journal of Applied Sciences, Vol. 7, Issue 8 · 31 Aug 2010 · 7,235 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioethanol Production from Rice StrawNutawan Yoswathana, Phattayawadee Phuriphipat, Pattranit Treyawutthiwat, Mohammad Naghi EshtiaghiProblem statement: Rice straw has been treated with different chemical (acid, alkali) and physical (subcritical water, ultrasound) methods to convert lignocellulose material to sugar. In addition enzyme treatment of pretreated samples has been applied to improve the conversion of lignocellulose material in sugar. Approach: Sulfuric acid at concentration of 1-9% was applied for acid treatment. For alkali treatment was sodium … …Energy Research Journal, Vol. 1, Issue 1 · 30 Jun 2010 · 18,056 accessesView PDFCite
Research ArticleOpen accessIn the abstractAlkali Pretreatment and Enzymatic Hydrolysis of Cattails from Constructed WetlandsBo Zhang, Abolghasem Shahbazi, Lijun Wang et al.Problem statement: To date, production of liquid fuel, particularly ethanol, has only been economically feasible from food crops that are high in sugar and starch. However, the use of arable land for fuel rather than for food production and the use of a food source for fuel rather than as food have created issues in prices and availability of traditional … …American Journal of Engineering and Applied Sciences, Vol. 3, Issue 2 · 30 Jun 2010 · 9,105 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71Farouk Al-Quadan, Hazem Akel, Rasha NatshiProblem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,400 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Thermostable α-Amylase Producing Natural Variant of Bacillus spp. Isolated From Soil in IranIraj Rasooli, Shakiba D. A. Astaneh, Hojjat Borna, Kamal A. BarchiniThermophilic processes appear more stable, rapid and less expensive and facilitate reactant activity and product recovery. Amylases have a quarter of the world enzyme market and thermostable a-amylases possess extensive commercial applications. Since little work has been done on strain isolation, growth and enzyme yield optimization, the level of thermophilic enzyme production remains relatively low. Therefore, large scale exploitation of … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 3 · 30 Sep 2008 · 8,902 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimum Conditions for Measuring Dehydrogenase Activity of Aspergillus niger using TTCA. E. Ghaly, N. S. MahmoudThe suitability of the triphenyl tetrazolium chloride (TTC) for dehydrogenase activity measurement of the vegetative cells of the fungal species Aspergillus niger was investigated. The triphenyl formazan (TF) yield increased with the increases in TTC concentration, pH, temperature and incubation time within the studied ranges. The effects of individual parameters, as well as the combined effects, on the TF yield … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 9,002 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,690 accessesView PDFCite
Research ArticleOpen accessIn the abstractDiversity and Allelopathic Potential of Weeds among Panamanian Coffee CropsLilia Cherigo, Jorge Lezcano, Sergio Martínez-LuisWorldwide, coffee is one of the most highly consumed and produced crops. Coffee production is a significant activity in the Panamanian economy, mainly in rural communities and among indigenous groups in the Chiriquí province highlands. Weeds growing alongside coffee plants can provoke considerable economic losses for producers by interfering with the growth, development and yield of coffee crop in cultivated … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 6 Sep 2017 · 7,465 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Hydrolysis Conditions for the Production of Iron-Binding Peptides from Scad (Decapterus maruadsi) Processing ByproductsWenting Zhang, Yanan Li, Jie Zhang, Guangrong HuangThe scad (Decapterus maruadsi) processing byproduct (SPB) was hydrolyzed by four commercial enzymes, namely, trypsin, flavourzyme, protamex and alcalase, for preparing high Iron-Binding Capacity (IBC) hydrolysate. Alcalase was the best choice for obtaining high IBC hydrolysate from SPB. Response surface methodology using a central-composite design was employed to optimize the enzymatic hydrolysis conditions with alcalase to obtain a maximum hydrolysate … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 17 Oct 2016 · 7,589 accessesView PDFCite
Research ArticleOpen accessIn the abstractOPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORMNayera A.M. Abdelwahed, Enas N. Danial, Noura El-Ahmady El-Naggar, Asem A. MohamedOptimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL-1 were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L-1 as carbon source and yeast extract 5 g L-1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Dec 2013 · 8,125 accessesView PDFCite