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130 results for “enzyme activity”
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Research ArticleOpen accessIn the abstractDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,117 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,937 accessesView PDFCite
Research NotesOpen accessIn the abstractAdvance in the Diagnostics of Mare Pregnancy and Assessing the Activity of Gonadotropin in Serum of in-Foal MareKaliya Kudaikulovna Yerkekulova, Nuradin Alibayev, Yusupzhan Artykovich Yuldashbayev et al.Research works on the improvement of mare pregnancy diagnostics and evaluation of biological activity of gonadotropic preparation based on the use of enzyme-linked immunosorbent assay were carried out. As the result of diagnostics and evaluation, effective producers as donors for obtaining gonadotropic preparation among the Kazakh population of mares with type “zhabe”, the animal units of 60 days with their … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 7 Mar 2024 · 4,830 accessesView PDFCite
Research ArticleOpen accessIn the abstractInvestigation of the Contribution of Late Embryogenesis Abundant (LEA) K Peptide in Enhancing the Expression of Lipase from Sphingobacterium sp.: In vitro and in Silico StudiesIbrahim Muhammad et al.Sphingobacterium sp. AB3 lipase is a psychrophilic enzyme with optimal lipolytic activity at pH 7 and 15°C. The cold-adapted properties of the lipase render it suitable for various low-temperature industrial applications such as biodiesel production, detergent formulation, and non-thermal food processing. Recent studies have shown that co-expression of LEA K peptide with AB3 lipase resulted in enhanced protein expression in … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 21 Nov 2024 · 4,572 accessesView PDFCite
Research ArticleOpen accessIn the abstractMaximizing Fungal Growth in State-of-the-Art Tray Solid-State Fermenter: Aeration Strategies Optimized for Enhanced PerformanceMusaalbakri Abdul Manan, Colin WebbThis study introduced a tray fermenter, featuring eight layers of squared perforated trays, specifically designed for solid-state fermentation applications. It is named the multi-layer squared tray solid-state fermenter. The fermenter employs two aeration strategies with varying air flow rates to optimize fungal growth. Firstly, sterilized moistened air at 8 L/min flows from the bottom through the perforated trays. Secondly, a … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 9 Apr 2024 · 6,462 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on GlycerolRuixue Wang, Feifei Xin, Yufei Chang et al.More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry of lipid accumulation in oleaginous microorganisms under nitrogen limitation has been extensively studied. As an oleaginous microorganism, Yarrowia lipolytica can produce fatty acid-derived biofuels and biochemicals. However, the response of the enzymes in Y. lipolytica to glycerol remains unclear. Therefore, we aimed … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 2 · 17 Apr 2023 · 6,316 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Decreasing Temperature on Soil Microbial Activity in a Boreal Shield EcozoneAlistar Moy, Kabwe NkongoloAlthough the focus on warming is imperative to address climate change, addressing the effects of cooling is arguably just as relevant in characterizing the enzyme response to temperature fluctuations. In this study, the response of enzyme activity to diminishing temperatures in the field was evaluated. The targeted sites were located in the City of Greater Sudbury in Northern Ontario, Canada. … …American Journal of Environmental Sciences, Vol. 18, Issue 2 · 17 Apr 2022 · 6,665 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Constituents and Bacteriostatic Activity of Essential Oil Extracted from Sea Buckthorn Seeds by Different MethodsHepeng Zhao, Fuyin Zhao, Qian Xiao et al.Sea buckthorn is a homologous plant listed in Chinese pharmacopoeia. The Essential Oils (EOs) of Sea Buckthorn Seeds (SBTS) are widely used in medicine and health products in China, Russia and other countries. The extraction methods of SBTS with advantages of low cost, high yield and high activity have attracted considerable attention. In this study, the extraction rate, chemical compositions … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 2 · 4 Jun 2021 · 7,483 accessesView PDFCite
Research ArticleOpen accessIn the abstractMicroflora Contribution to Cellulase and Digestion Enzyme in Mud Crab Digestive TractSiti Aslamyah, Yushinta Fujaya, Nita RukminasariMicroflora in the digestive tract of aquatic animals is thought to be not only the microbes that contribute to the production of cellulase enzymes but also contribute to other digestive enzymes, such as protease, amylase and lipase. This study aims to examine the microflora contribution to the mud crab’s digestive tract. Scylla sp. were maintained in the crab box individually … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 26 Mar 2021 · 6,456 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuantitative Analysis of Hypothalamic Proline-Rich Peptide-1 in Blood Serum of Patients with Malignant TumorsNarine Tumasyan, Silva Abrahamyan, Nune Kocharyan et al.The proline-rich-peptides are a class of neurosecretory hypothalamic bioactive polypeptides derived from cattle and comprise the content of bovine posterior pituitary neurosecretory granules. These peptides are co-validated through a single precursor protein neurophysin-vasopressin-associated glycoprotein. Proline-Rich-Polypeptide-1 (PRP-1) is a 15-amino acid peptide with anti-neurodegenerative, immunoregulatory, hematopoietic, antimicrobial, and antitumor activity. The aim of this study, accordingly, is to elaborate an assay … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 27 Nov 2025 · 2,287 accessesView PDFCite