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147 results for “chromatography”
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Research ArticleOpen accessIn the keywordsAutomatic Identification of Molecules in a Thin-Film Extract Combined with LaserTheodore Guié Toa Bi, Sié Ouattara, Alain ClementThis paper presents the automation of the thin-layer chromatography technique whose separation and identification of molecules present in a mixture are currently done manually and laboriously. We have therefore found an interest in automating this technique. In this part, the method implemented comprises 2 steps. First we proceeded to the segmentation of the spots obtained on the chromatographic plate. We … …American Journal of Engineering and Applied Sciences, Vol. 11, Issue 4 · 14 Nov 2018 · 7,089 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSimultaneous Detection of Nitrite and Nitrate in Celeries and CarrotsDongwu Liu, Jinye Niu, Ling Kong, Zhiwei ChenIn this study, the anions nitrite ion (NO2-) and nitrate ion (NO3-) in celeries and carrots were detected with the technique of ion chromatography. A Dionex ICS-2000 ion chromatograph was used to detect the content of NO2- and NO3- in celeries and carrots. NO2- and NO3- were separated on a DIONEX Ionpac AS19 A-4 mm ion-exchange column and an Iopac … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 14 May 2016 · 7,214 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetermination of Abscisic Acid and its Relationship to Drought Stress Based on Cowpea Varieties with Different Capability of Drought ResistanceWei Lei, Shian Huang, Shaohu Tang et al.Cowpea (Vigna unguiculata L.) is one of the most important grain legume supplying necessarily dietary proteins. Although its can tolerate drought environment, still the severe condition can limit the growth and development of this plant. Abscisic acid (ABA) has a crucial function in stress physiology, but little information is available for methods describing ABA determination in cowpea and its relationship … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 30 Mar 2016 · 8,458 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInfluence of Ethanol and Methyl Groups on the Degradation of Xylenes in SoilIrajá Nascimento, Ricardo Baldasso, Kira ManfrediniThe main objective of the present study was to assess the influence of ethanol on the evaporation profile of xylenes (ortho + meta and para isomers) in active (with microorganisms) and inert (without microorganisms) soils. The vapors from four sealed flasks containing inert soil + neat xylenes, active soil + neat xylenes, inert soil + xylenes + ethanol and active … …American Journal of Applied Sciences, Vol. 12, Issue 6 · 25 Jul 2015 · 6,622 accessesView PDFCite
Research ArticleOpen accessIn the keywordsNATURAL ANTIOXIDANT INGREDIENT FROM BY-PRODUCTS OF FRUITSG. S. El-Baroty, M. F. Khalil, S. H.A. MostafaThe contents of total phenolics compounds and their phenolic constituents were quantified in organic and aqueous of four varieties (Zebdia, Sukkari, taimor and Hindi) of mango (Mangifera indica L.), seeds pulp and kernel, one varieties of pomegranate (Punica ranatum L., peel) and peanut (Arachis hypogaea L., Giza 6, shell) by-products. The antioxidant activities of all by products extracts were assessed … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 22 Mar 2014 · 8,170 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEXTRACTION OF QUERCETIN FROM POLYGONUM HYDROPIPER L. BY SUBCRITICAL WATERA. V. Lekar, S. N. Borisenko, E. V. Vetrova et al.The new method of quercetin extraction from Polygonum hydropiper L. by subcritical water was developed. High performance liquid chromatography was used for identification and quantification of flavonoids in the extract. The new method is environmentally friendly and more effective (7.6-times) than traditional flavonoids extraction methods using expensive and toxic organic solvents. …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 1 · 23 Nov 2013 · 8,612 accessesView PDFCite
Research ArticleOpen accessIn the keywordsRETRACTED: Analysis of Interfering Substances in the Measurement of Malondialdehyde Content in Plant LeavesYunSheng Wang, MaoDi Ding, XunGang Gu et al.The Thiobarbituric Acid (TBA) reactive substances assay is an easy and quick assay for the assessment in plants of lipid peroxidation, in which Malondialdehyde (MDA) is derivatized. To analyze the applicability of this method, the MDA concentrations in the leaves of different plants were measured by the TBA method. To further separate the interfering substances, fractional extraction and Gas Chromatography-Mass … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 9,288 accessesView PDFCite
Research ArticleOpen accessIn the keywordsResidues Depletion Study and Withdrawal Period Determination of Sulphadiazine and Trimethoprim Premix in PigsPanagiotis Dimitrios Katsoulos, Lydia Dritsa, Georgios Christodoulopoulos et al.The emergence and spread of antibiotic resistant human pathogens have been directly linked to the use of antibiotics in livestock production. The purpose of this study was to detect and quantify the concentration of the residues of Sulphadiazine (SDZ) and Trimethoprim (TMP) in edible tissues of pigs and to determine the withdrawal period after oral administration of OPTIPRIME® premix 40%, … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 1 · 12 Feb 2013 · 8,360 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEmerging Trends in Designing Short and Efficient Protein Purification ProtocolsSaurabh Gautam, Joyeeta Mukherjee, Ipsita Roy, Munishwar Nath GuptaProtein purification is carried out in both academic and industrial sectors. Both non-chromatographic approaches (precipitation, crystallization, partitioning) and chromatographic methods are used. When recombinant proteins are over expressed in foreign hosts, inclusion bodies are obtained. Many purification methods can be used to refold proteins. Many strategies allow one to avoid formation of inclusion bodies. The concept of affinity based separation … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 9,007 accessesView PDFCite
Research ArticleOpen accessIn the keywordsFORMULATION AND CLINICAL TESTING OF GLYCERYL TRINITRATE OINTMENT FOR TREATMENT OF ANAL FISSURESEmad Mothana Yousif, Harith Jameel Mahdi, Ali Ibrahem Khaleel, Shathel K. NoamanUntil know surgical intervention considered to be the cornerstone for treatment of anal fissure condition. Regarding all the circumstances that hinder the possibility of accomplishment of surgical operation and the possible post-urgical complications; all that motivates the necessity for a non-surgical procedure for treatment of anal fissure. This study was designed to establish a pharmaceutically active and chemically and physically … …American Journal of Pharmacology and Toxicology, Vol. 7, Issue 3 · 1 Sep 2012 · 8,965 accessesView PDFCite