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Research ArticleOpen accessIn the keywordsSimultaneous Detection of Nitrite and Nitrate in Celeries and CarrotsDongwu Liu, Jinye Niu, Ling Kong, Zhiwei ChenIn this study, the anions nitrite ion (NO2-) and nitrate ion (NO3-) in celeries and carrots were detected with the technique of ion chromatography. A Dionex ICS-2000 ion chromatograph was used to detect the content of NO2- and NO3- in celeries and carrots. NO2- and NO3- were separated on a DIONEX Ionpac AS19 A-4 mm ion-exchange column and an Iopac … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 14 May 2016 · 7,214 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA screen for Benzo(a)pyrene in Fish Samples From Crude Oil Polluted EnvironmentsChimezie Anyakora, Mohsen Arbabi, Herbert CokerSeveral studies have shown that exposure to Benzo(a)pyrene increases the risk of cancer. In this study several fish samples from Niger the Delta region of Nigeria were screened for the presence of Benzo(a)pyrene. The study was carried out using Gas chromatograph coupled to a mass spectrometry detector. Benzo(a)pyrenes in the samples were identified through both retention time match with authentic … …American Journal of Environmental Sciences, Vol. 4, Issue 2 · 30 Apr 2008 · 6,766 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAcer Buergerianumas a New Resource for the Extraction of Corilagin: Determination of Corilaginin Different Cultivars of A. Buergerianumby UPLCQian Qiao, Chong Wu, Jiangyong Wang et al.Corilagin is a promising anticancer drug candidate. Acer nikoense and A. ammonium are both known to contain corilagin. To explore whether corilagin occurs in congeneric species and to fully develop the medicinal value of maple, the corilagin content in different varieties of maple was determined by Ultrahigh-Performance Liquid Chromatography (UPLC). Acer buergerianum leaves contained corilagin and the corilagin content in … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 2 · 12 May 2022 · 5,704 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,097 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAutomatic Identification of Molecules in a Thin-Film Extract Combined with LaserTheodore Guié Toa Bi, Sié Ouattara, Alain ClementThis paper presents the automation of the thin-layer chromatography technique whose separation and identification of molecules present in a mixture are currently done manually and laboriously. We have therefore found an interest in automating this technique. In this part, the method implemented comprises 2 steps. First we proceeded to the segmentation of the spots obtained on the chromatographic plate. We … …American Journal of Engineering and Applied Sciences, Vol. 11, Issue 4 · 14 Nov 2018 · 7,089 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetermination of Abscisic Acid and its Relationship to Drought Stress Based on Cowpea Varieties with Different Capability of Drought ResistanceWei Lei, Shian Huang, Shaohu Tang et al.Cowpea (Vigna unguiculata L.) is one of the most important grain legume supplying necessarily dietary proteins. Although its can tolerate drought environment, still the severe condition can limit the growth and development of this plant. Abscisic acid (ABA) has a crucial function in stress physiology, but little information is available for methods describing ABA determination in cowpea and its relationship … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 30 Mar 2016 · 8,458 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInfluence of Ethanol and Methyl Groups on the Degradation of Xylenes in SoilIrajá Nascimento, Ricardo Baldasso, Kira ManfrediniThe main objective of the present study was to assess the influence of ethanol on the evaporation profile of xylenes (ortho + meta and para isomers) in active (with microorganisms) and inert (without microorganisms) soils. The vapors from four sealed flasks containing inert soil + neat xylenes, active soil + neat xylenes, inert soil + xylenes + ethanol and active … …American Journal of Applied Sciences, Vol. 12, Issue 6 · 25 Jul 2015 · 6,622 accessesView PDFCite
Research ArticleOpen accessIn the keywordsNATURAL ANTIOXIDANT INGREDIENT FROM BY-PRODUCTS OF FRUITSG. S. El-Baroty, M. F. Khalil, S. H.A. MostafaThe contents of total phenolics compounds and their phenolic constituents were quantified in organic and aqueous of four varieties (Zebdia, Sukkari, taimor and Hindi) of mango (Mangifera indica L.), seeds pulp and kernel, one varieties of pomegranate (Punica ranatum L., peel) and peanut (Arachis hypogaea L., Giza 6, shell) by-products. The antioxidant activities of all by products extracts were assessed … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 22 Mar 2014 · 8,170 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEXTRACTION OF QUERCETIN FROM POLYGONUM HYDROPIPER L. BY SUBCRITICAL WATERA. V. Lekar, S. N. Borisenko, E. V. Vetrova et al.The new method of quercetin extraction from Polygonum hydropiper L. by subcritical water was developed. High performance liquid chromatography was used for identification and quantification of flavonoids in the extract. The new method is environmentally friendly and more effective (7.6-times) than traditional flavonoids extraction methods using expensive and toxic organic solvents. …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 1 · 23 Nov 2013 · 8,612 accessesView PDFCite
Research ArticleOpen accessIn the keywordsRETRACTED: Analysis of Interfering Substances in the Measurement of Malondialdehyde Content in Plant LeavesYunSheng Wang, MaoDi Ding, XunGang Gu et al.The Thiobarbituric Acid (TBA) reactive substances assay is an easy and quick assay for the assessment in plants of lipid peroxidation, in which Malondialdehyde (MDA) is derivatized. To analyze the applicability of this method, the MDA concentrations in the leaves of different plants were measured by the TBA method. To further separate the interfering substances, fractional extraction and Gas Chromatography-Mass … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 9,288 accessesView PDFCite