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32 results for “Saccharomyces sp.”
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Research ArticleOpen accessIn the abstractThe Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in Pichia pastoris KM71Popi Asri Kurniatin, Fifi Fitriyah Masduki, Neni Nurainy et al.A spacer peptide (Glu-Ala repeats) connects N-terminal of a polypeptide precursor to the signal peptide of α-mating factor (α-MF) from Saccharomyces cerevisiae. During the polypeptide precursor secretion, the signal peptide will be first cleaved by signal peptidase in the endoplasmic reticulum, followed by Kex2 endopeptidase and finally the spacer peptide is removed by dipeptidyl aminopeptidase in the Golgi apparatus. In … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 31 Oct 2019 · 9,026 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,097 accessesView PDFCite
Research ArticleOpen accessIn the abstractEthanol Production by Novel Proline Accumulating Pichia kudriavzevii Mutants Strains Tolerant to High Temperature and Ethanol StressesRika Indri Astuti, Sena Alifianti, Ratu Nabila Maisyitoh et al.Accumulation of osmoprotectant molecule, proline, has been reported to induce a stress tolerance phenotype in yeast cells. In this study, two ethanologenic isolates of yeast, Pichia kudriavzevii (R and T), both capable of using both five and six sugar compounds to produce ethanol, were mutated via ethyl methanesulfonate (EMS) treatment. Prolineaccumulating mutant strains were selected by using proline-analogue (L-azetidine-2-carboxylic acid). … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 11 Sep 2018 · 7,838 accessesView PDFCite
Review ArticleOpen accessIn the abstractThe use of Probiotics in Horses with Gastrointestinal DiseaseJelena Gotić, Darko Grden, Nikica Prvanović Babić, Vladimir MrljakProbiotics are common adjunctive therapy used in human and veterinary medicine, mostly intended for gastrointestinal diseases treatment or prevention. Lactic acid bacteria: Lactobacilli, bifid bacteria and enterococci and Saccharomyces boulardii, a subtype of the nonpathogenic yeast have been most frequently used as probiotic active ingredients. To become a recognized effective probiotic, a certain microorganism must fulfill many expectations, primarily the … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 3 · 30 Aug 2017 · 8,679 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification and Characterization of Diacylglycerol Acyltransferase in Oleaginous Yeast Rhodosporidium toruloidesZhen Wang, Huaiyuan Zhang, Lina Zhao, Yuanda SongDiacylglycerol acyltransferase (DGAT), which catalyzes TAG formation from DAG and acyl-CoA, has been considered to play a vital role in TAG accumulation in oleaginous microorganisms. The genome of oleaginous yeast Rhodosporidium toruloides contains two putative DGAT genes, RtDGATa and RtDGATb, which shared little conserved amino acid coding sequence with each other. Phylogeny tree analysis showed that RtDGATa belonged to DGAT1 … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 17 Oct 2016 · 7,387 accessesView PDFCite
EditorialOpen accessIn the abstractAre Heat Shock Proteins Druggable Candidates?Tawanda Zininga, Addmore ShonhaiBanumathy, G., V. Singh, S.R. Pavithra and U. Tatu, 2003. Heat shock protein 90 function is essential for Plasmodium falciparum growth in human erythrocytes. J. Biol. Chem., 278: 18336-18345. DOI: 10.1074/jbc.M211309200 Brehmer, D., S. Rudiger, C.S. Gassler, D. Klostermeier and L. Packschies et al., 2001. Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange. Nature Structural Molecular … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 4 · 2 Dec 2014 · 8,166 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Microbial Inoculants on Feeding Value of Spent Lentinula edodes SubstrateYunfu Gu, Min Yi, Jianzhou Chen, Xiaoping ZhangSawdust-based Spent Lentinula Edodes Substrate (SLES) is an important agricultural waste resource for its’ huge production amount, on the other hand, it is hard to recycling because of the low digestibility. For the purpose of recycling the SLES, a study was conducted to improve the feeding values of SLES via microbial inoculation. The SLES was ensiled with 0.5% (v/w) Lactic … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 4 · 20 Sep 2012 · 7,911 accessesView PDFCite
Research ArticleOpen accessIn the abstractEfficient Anaerobic Fermentation of Simple Sugars by Yeast Fuels Resistance Candida spp. Infections to Eradication by DrugsNedosa Ikenna Valentine, Utoh-Nedosa Uchechukwu Anastasia, Dan Danjuma et al.Problem statement: Human systemic Candida infections had proved difficult to eradicate by the medical health care system. Some practitioners and scholars see the problem as being due to drug resistance. For example an author wrote that ‘secondary drug resistance is clearly being encountered in one setting, oropharyngeal candidiasis in patients with advanced Human Immunodeficiency Virus type 1 (HIV-1) infection usually … …American Journal of Infectious Diseases, Vol. 7, Issue 4 · 10 Dec 2011 · 7,836 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioethanol Production from Rice StrawNutawan Yoswathana, Phattayawadee Phuriphipat, Pattranit Treyawutthiwat, Mohammad Naghi EshtiaghiProblem statement: Rice straw has been treated with different chemical (acid, alkali) and physical (subcritical water, ultrasound) methods to convert lignocellulose material to sugar. In addition enzyme treatment of pretreated samples has been applied to improve the conversion of lignocellulose material in sugar. Approach: Sulfuric acid at concentration of 1-9% was applied for acid treatment. For alkali treatment was sodium … …Energy Research Journal, Vol. 1, Issue 1 · 30 Jun 2010 · 18,056 accessesView PDFCite
Research ArticleOpen accessIn the abstractAlkali Pretreatment and Enzymatic Hydrolysis of Cattails from Constructed WetlandsBo Zhang, Abolghasem Shahbazi, Lijun Wang et al.Problem statement: To date, production of liquid fuel, particularly ethanol, has only been economically feasible from food crops that are high in sugar and starch. However, the use of arable land for fuel rather than for food production and the use of a food source for fuel rather than as food have created issues in prices and availability of traditional … …American Journal of Engineering and Applied Sciences, Vol. 3, Issue 2 · 30 Jun 2010 · 9,105 accessesView PDFCite