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291 articles for “PCR”
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Research ArticleOpen accessEvery word in the titleCharacterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCRMohamed A. Nagaty, Salah El-Din El-Assal, Mahmoud M. RifaatProblem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, flowering and fruiting indices were determined and eight, 10-base primers were used to … …American Journal of Applied Sciences, Vol. 8, Issue 7 · 24 Jun 2011 · 7,271 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEvaluation of Nested PCRs targeting the BI and SAG2 Genes For Detection of Toxoplasma gondii Genome in Aqueous Humor From HIV Positive Toxoplasma Retinochoroiditis Patients in a Tertiary Eye HospitalB. Mahalakshmi, K.L. Therese, R. Kirthika et al.Abstract: Problem statement: To evaluate nPCR targeting B1 gene (primer set 1) with 3 other nPCRs targeting B1 gene (primer set 2), 3' end of SAG2 and 5' end of SAG2 gene, for detection of Toxoplasma gondii (T. gondii) genome on AH from HIV positive TRC patients. Approach: DNA extracted from AH of 12 TRC patients and 12 controls (patients … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Dec 2010 · 6,886 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Cross-Sectional Study of the Prevalence of Hepatitis C Virus InfectionOmar Atrooz, Rehab Thunibat, Ihssan AtroozLiver pain is increased by hepatotropic viruses, such as the Hepatitis C Virus (HCV). This illness can progress to cirrhosis and hepatocellular cancer. This study aims to determine the prevalence of hepatitis C in the Southern Jordanian cities (Karak and Tafilah) as well as the correlations between alanine Aminotransferase (ALT), Aspartate aminotransferase (AST), age and gender in hepatitis C patients. … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 5 Jul 2024 · 4,414 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDifferential Gene Expression of Peroxide Resistant Protein (AhpC) in Planktonic and Biofilm State of Uropathogenic Escherichia coli CellsOmar Sadik Shalal, Ani-Simona SevastreThe gastrointestinal tract seems to be the primary reservoir of Uropathogenic Escherichia coli (UPEC) in humans. UPEC strains harbor the Urinary Tract (UT) and cause Urinary Tract Infections (UTI) which might represent a serious threat to human life. To counteract with the damage caused by the Reactive Oxygen Species (ROS), bacterial strains produce various enzymes and proteins like Alkyl hydroperoxide … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 3 · 13 Oct 2023 · 5,324 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEstablishment and Application of Rapid Quantitative Detection Technology for Bacillus cereusFeilong Sun, Hanyu Li, Jingxue Qian et al.Infections caused by Bacillus cereus often occur, causing a certain degree of health hazards and economic losses to people around the world. Rapid quantitative detection is of great significance for prevention. This study aims to develop a simple and rapid quantitative detection method for B. cereus, which can be used to monitor the contamination of B. cereus in food samples. … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 21 Mar 2023 · 5,564 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMycobacterium tuberculosis Complex Mutations in Drug Resistant Clinical Isolates from Southwest MexicoMartínez-Cruz Perla Mónica, Nakamura-López Yuko, Quintero-Hernández Verónica et al.Mutations in target genes have been described in Mycobacterium tuberculosis Complex (MTBc) drug resistant isolates worldwide. In Mexico, not enough information has been reported in this concern. The aim of this study was to characterize mutations related to resistance to first line drugs in MTBc isolates from Oaxaca, Mexico. MTBc isolates were identified in clinical samples from Tuberculosis (TB) patients. … …American Journal of Infectious Diseases, Vol. 17, Issue 4 · 28 Oct 2021 · 6,667 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPrevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, AlgeriaSabrina Rabehi, Bakir Mamache, Taha Mossadak Hamdi et al.The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 15 Oct 2021 · 6,997 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMarking of Meat Productivity Features in Pairs of bGH, bGHR and bIGF-1 Polymorphic Genes in Aberdeen-Angus CattleLaura Zhanedilovna Dushayeva, Askar Myrzahmetovich Nametov, Indira Saltanovna Beishova et al.The purpose of the study is to identify paired combinations associated with meat productivity in Aberdeen-Angus cattle for the genes of the somatotropin cascade (growth hormone bGH, growth hormone receptor bGHR, insulin-like growth factor-1 bIGF-1). The determination of animal genotypes was carried out by PCR-RFLP. Results of statistical processing was carried out using the software capabilities of "Microsoft Excel 2010" … …OnLine Journal of Biological Sciences, Vol. 21, Issue 2 · 22 Jun 2021 · 6,899 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Identification of Leishmania Species in Patient with Cutaneous Leishmaniasis in an Endemic Area of Fars Province, Southern IranVahid Rahmanian, Nader Sharifi, Zahra Kargar Jahromi et al.The Cutaneous Leishmaniasis (CL) contains a range of factors, which provide different patterns of transmission that make it difficult to control. The aim of this study was to identify the different species of Leishmania in the Jahrom district of Fars province, southern Iran. This descriptive study was done on CL suspected cases who were referential to the Reference of Jahrom … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 2 · 2 Apr 2021 · 6,944 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSerological and Molecular Investigation of Middle East Respiratory Syndrome Coronavirus and Equine Herpesviruses in Horses at Southwestern Saudi ArabiaAli S. AlqahtaniHost reservoirs other than dromedaries for the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) still to be unknown. Equine Herpesviruses (EHVs) are widespread in equine populations worldwide. In this study, the presence of MERS-CoV, Equine Herpesviruses type 1 (EHV-1) and 4 (EHV-4) in horses in Najran, southwestern of Saudi Arabia was investigated. One hundred and thirty four blood samples collected from … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 3 · 29 Sep 2020 · 6,602 accessesView PDFCite