31 results for “Nucleotide Sequence”

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Research ArticleOpen accessIn the abstractPyrosequencing-An Alternative to Traditional Sanger SequencingMd Fakruddin, Abhijit ChowdhuryProblem statement: Pyrosequencing has the potential to rapidly and reliably sequence DNA taking advantages over traditional Sanger di-deoxy sequencing approach. Approach: A comprehensive review of the literature on the principles, applications, challenges and prospects of pyrosequencing was performed. Results: Pyrosequencing was a DNA sequencing technology based on the sequencing-by-synthesis principle. It employs a series of four enzymes to accurately detect … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 1 · 2 Mar 2012 · 12,887 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDF Cite

Research ArticleOpen accessIn the abstractVibrio cholerae O1 Strains of Different Ribotypes have Similar hlyA RFLP Patterns but Different Vacuolating AbilityJorge E. Vidal, Paula Figueroa-Arredondo, Silvia Giono-Cerezo et al.Extensive cytoplasmic vacuolation on Vero and HeLa cells in vitro by the Vibrio cholerae pore forming toxin HlyA, has been previously reported by our group. Vibrio cholerae O1 and non-O1 pathogenic strains show differences in the potential to induce vacuolation, here we study occurring variations on vacuolating cytotoxic ability, related to changes in the nucleotide sequence of the hlyA-orf. A … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,984 accessesView PDF Cite

Research ArticleOpen accessIn the abstractToll-Like Receptor (TLR) 2 and TLR4 Gene Expression in Canine HeartA. Linde, F. Blecha, T. MelgarejoToll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,921 accessesView PDF Cite

Research ArticleOpen accessIn the abstractMolecular Characterization of Vaccine-Derived Mutants of Avian Metapneumoviruses Isolated from Turkeys in EgyptAbdel-Satar Arafa, Wessam Mady, Ahmed Hussein et al.Avian metapneumovirus (aMPV) causes acute respiratory infection in turkeys with sudden onset and rapid spread in the infected flocks. Vaccinated flocks frequently showed clinical signs typical like the non-vaccinated flocks in Egypt, this situation suggests a virulent vaccine virus was circulating in the field. Oropharyngeal and nasal sinus swabs were collected from 100 turkeys from 10 commercial farms with mild … …Current Research in Virology, Vol. 4, Issue 1 · 12 Aug 2015 · 12,748 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBioinformatics Analysis of Xyloglucan Endotransglycosylase/Hydrolase (XTH) Gene from Developing Xylem of a Tropical Timber Tree Neolamarckia CadambaTiong Shing Yiing, Ho Wei Seng, Pang Shek Ling, Ismail JusohThis study reported the isolation and in silico characterization of a full-length Xyloglucan endotransglycosylase/Hydrolase (XTH) cDNA from Neolamarckia cadamba, an important tropical light hardwood plantation tree species. XTH is considered as a key agent to regulate cell wall expansion and is believed to be responsible for the incorporation of newly synthesised xyloglucan into the wall matrix. The full-length of NcXTH … …Current Research in Bioinformatics, Vol. 3, Issue 1 · 6 Jan 2015 · 7,426 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPHYLOGENETIC ANALYSIS OF THE SWINE LEUKOCYTE ANTIGEN-6 GENE GENERATED FROM SPLEENTISSUE OF KOREAN NATIVE PIGJaeyoung Kim, Hyojun Park, Yoonchung Choi, Hoyoung ChungThis study was aimed to search genetic variants, to investigate phylogenetic relationships between pig breeds and to provide basic genetic information of Korean Native Pigs (KNP) using the variations of the Swine Leukocyte Antigen-6 (SLA-6) gene. Cloning of the Swine Leukocyte Antigen (SLA)-6 gene in the MHC non-classical region was performed with spleen tissues of Korean Native Pigs (KNP). Sequencing … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 22 Feb 2014 · 7,234 accessesView PDF Cite

Research ArticleOpen accessIn the abstractFirst Report of Human Immunodeficiency Virus Type 1 Circulating Recombinant Form 02_AG Recombinant Strain of African Origin from North IndiaUjjwal Neogi, Vikas Sood, Arpita Choudhury et al.Problem statement: Human Immunodeficiency Virus Type-1 (HIV-1) displays extensive genetic diversity globally which poses a serious challenge in making efficacious vaccine and developing effective therapeutic interventions. HIV-1 epidemic in India is largely driven by genetic subtype C but lately a number of reports suggest the presence of circulating recombinant forms and unique recombinant forms from different states of India. Approach: … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Oct 2010 · 6,737 accessesView PDF Cite

Research ArticleOpen accessIn the abstractHormetic Triggers for Intervention in Aging, Disease and TraumaJoan Smith SonnebornHormesis refers to the ability of a chemical or physical agent to condition the physiological state of an organism to tolerate stress with low doses of otherwise harmful agents. Evolutionary evidence shows that the survival and longevity of species hinge on their optimal ability to resist stress challenge. Hormesis is a potent strategy to stimulate latent repair processes to tolerate … …American Journal of Pharmacology and Toxicology, Vol. 3, Issue 1 · 31 Mar 2008 · 7,895 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordAlcohol Intubation and Synergistic Effect of Folic Acid and Egg Yolk Powder Solution on Alcohol Dehydrogenase [ADH] Class I Gene Expression and Single Nucleotide Polymorphism Frequency in Parent and Prenatal Alcohol Exposed Wistar RatsAdejoke Olukayode Obajuluwa, Oluyinka Ajibola Iyiola, Joseph Akintade Morakinyo et al.Point mutation detections in alcohol metabolizing genes could unravel molecular targets of gestational alcohol and epigenetic remodeling capacity of maternal nutrition, which underscores growth and health endpoints in Prenatal Alcohol Exposed (PAE) fetuses. gene expression was assayed using sequenced target and internal control gene primers in a two-step thermal cycling process from isolated RNA in purely bred alcohol male and … …OnLine Journal of Biological Sciences, Vol. 24, Issue 1 · 22 Nov 2023 · 4,857 accessesView PDF Cite