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68 articles for “HPLC-MS”
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Research ArticleOpen accessIn the keywordsThe Effects of Chinese Traditional Processing Method on Components in Semen Sinapis AlbaeLifang Liu, Hui Zhou, Suqin Sun et al.Techniques of infrared (IR) spectrophotometry, High Performance Liquid Chromatography (HPLC) and HPLC-mass spectrometry are employed in this study to reveal the changes of the components in Semen sinapis Albae during the Chinese traditional processing. While the degradation of proteins and polysaccarides can be observed, sinapine in this drug can be known to decompose immediately after 15 mins during processing. A … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,810 accessesView PDFCite
Research ArticleOpen accessIn the keywordsVitamin E and Beta Carotene Composition in Four Different Vegetable OilsAb. Gapor Md. Top, Halimah Muhamad, Aminah Abdullah et al.Problem statement: Some vegetable oils contains natural antioxidants such as beta carotene and vitamin E namely tocopherol and tocotrienol. Different vegetable oils contained different amount of vitamin E and β-carotene. Approach: Study was carried out to investigate the natural antioxidants (vitamin E and beta carotene) composition in four different vegetable oils [Red Palm Olein (RPO), palm plein (PO), Corn Oil … …American Journal of Applied Sciences, Vol. 8, Issue 5 · 17 May 2011 · 9,316 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffects of Vitamin A on Growth Performance, Antioxidant Status and Blood Constituents in Lactating Grey GoatWeiren Yang, Ping Wang, Yuanqiang Jing et al.Problem statement: It is regarded that vitamin A protects cells from damage by radicals which are believed to contribute to certain chronic diseases and regulates immune function of animals by protection of mucosal epithelium acting as the first defense barrier. All these properties of vitamin A may be attributable to its antioxidant activity. This study was to evaluate the effects … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 27 Dec 2010 · 7,674 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification, Structural Characterization and Anti-Aging and Stress Activities of Cordyceps Mycelium Polysaccharides from Paecilomyces HepialiXiaokang Hu, Huixiu Ma, Guicai Chen et al.This study identified and characterized the bioactive polysaccharide components from Cordyceps mycelium powder (CMP) derived from Paecilomyces hepiali, evaluating their anti-aging and stress-protective activities. Initial screening using Drosophila melanogaster revealed that crude Cordyceps polysaccharides (CCP) exhibited comparable effects to CMP and Cordyceps aqueous extracts (CAE) on natural lifespan and UV stress resistance, suggesting CCP as the primary bioactive component. CCP … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 16 Feb 2026 · 1,698 accessesView PDFCite
Research ArticleOpen accessIn the abstractUnveiling the Potential of Dombeya buettneri K.Schum As a Drug Agent: Molecular Docking of Compounds With Enzymes Linked to Diabetes and Neurodegenerative DiseaseEsther Emem Nwanna, Samuel Chima Ugbaja et al.This study investigated the mechanisms of Dombeya buettneri (snowball leafy) extracts on diabetes, hypertension, and neurodegenerative diseases. It compared extracts obtained using steep water (a by-product of corn wet milling) and aqueous water. The extracts were analyzed for total phenol, total flavonoid content, antioxidant activities such as FRAP, ABTS, NO, DPPH, iron-induced lipid peroxidation assay, degradation of deoxyribose (Fenton’s reaction), … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 1 Feb 2026 · 2,025 accessesView PDFCite
Research ArticleOpen accessIn the abstractPolygonatum sibiricum Red. With Steaming Yellow Rice Wine: Processing Technology, the Content of 5 Hydroxymethylfurfura and Safety AssessmentShiyu Ji, Shuo Li et al.To investigate the content and safety evaluation of 5 Hydroxymethylfurfural (5-HMF) in the processing of Polygonatum sibiricum Red, the steaming technology was optimized by response surface method combined with analytic hierarchy process of multiple indexes. The content of 5-HMF was quantitatively determined by HPLC. Safety assessment was conducted using a zebrafish model. The optimized conditions were a steaming time of … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 20 Nov 2025 · 2,587 accessesView PDFCite
Research ArticleOpen accessIn the abstractAcaricidal Activity of Azadirachta indica (Neem Tree) and Carica papaya (Papaya) Leaf Extracts against Rhipicephalus sanguineus (Dog Ticks) Using Spray MethodAlfredo Vita Corpuz, Carmela Montoya Florentino, Paul Erman Tabuloc Quilana et al.Ticks pose a serious threat to dogs, with synthetic acaricides leading to environmental impact and resistance concerns. While Azadirachta indica and Carica papaya have shown acaricidal potential against Rhipicephalus sanguineus, their efficacy against dog ticks has not been thoroughly investigated. This study determined the acaricidal activity of A. indica and C. papaya leaf extract against R. sanguineus, both individually and … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 22 Apr 2025 · 8,409 accessesView PDFCite
Research ArticleOpen accessIn the abstractSeparation, Purification and Anti-aging Activity of Tobacco PolysaccharidesMengyue Huang, Jingwu Song, Shuaishuai Chang et al.To separate tobacco polysaccharides and investigate their antioxidant and anti-aging properties, crude tobacco polysaccharides were extracted from raw tobacco using hot water, followed by purification through DEAE-52 cellulose column chromatography. The molecular weight and monosaccharide composition of the isolated polysaccharides were analyzed using HPGPC and HPLC. The antioxidant activities of these tobacco polysaccharides were tested using DPPH, ABTS, hydroxyl radicals … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 15 Jan 2025 · 5,103 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Ultra-High Pressure Technology on Isomerization and Antioxidant Activity of Lycopene in Solanum LycopersicumSiqun Jing, Zainixi Lasheng, Saisai Wang, Hui ShiTo evaluate the influence of the Ultra-High Pressure processing (UHP) on the isomerization and antioxidant activity of lycopene in tomatoes (Solanum lycopersicum), High Performance Liquid Chromatography (HPLC) and Ultraviolet-Visible Spectroscopy (UV-Vis) were used to separate and identify the isomer of lycopene. The scavenging activities against DPPH∙and∙OH present samples with and without UHP treatment were tested with ascorbic acid as positive … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 21 Jun 2020 · 7,225 accessesView PDFCite
Research ArticleOpen accessIn the abstractBlue and White LED Lights Enhance Biosynthesis of Rosmarinic Acid in Cell Culture of Agastache rugosaWoo Tae Park, Sun Kyung Yeo, Nam Su Kim et al.Agastache rugosa, an herbaceous Korean perennial, contains pharmacologically important phenylpropanoids such as tilianin and rosmarinic acid. However, the knowledge of biosynthesis of phenylpropanoids from A. rugosa is limited. Therefore, a study was conducted to develop an efficient protocol for in vitro regeneration of A. rugosa and to investigate the influence of three different LED light wavelengths (white, blue and red) … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 24 May 2020 · 6,860 accessesView PDFCite