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130 articles for “Enzyme Activity”
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Research ArticleOpen accessIn the abstractOptimization of Temperature and pH for Protease Production from Some Bacterial Isolates of Mangrove WatersFeskaharny Alamsjah, Yorasakhi Ananta, Anthoni AgustienProtease enzymes are widely used in biotechnology to produce amino acids and peptides from high molecular substrates, as well as in the leather industry for wastewater treatment, textiles, pharmaceuticals, cosmetics, leather processing, and poultry industry. Indonesia's demand for protease enzymes is increasing. This study aims to optimize the temperature and pH for the production of protease from certain bacterial isolates … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 11 Sep 2024 · 4,425 accessesView PDFCite
Research ArticleOpen accessIn the abstractDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,115 accessesView PDFCite
Research ArticleOpen accessIn the abstractMaximizing Fungal Growth in State-of-the-Art Tray Solid-State Fermenter: Aeration Strategies Optimized for Enhanced PerformanceMusaalbakri Abdul Manan, Colin WebbThis study introduced a tray fermenter, featuring eight layers of squared perforated trays, specifically designed for solid-state fermentation applications. It is named the multi-layer squared tray solid-state fermenter. The fermenter employs two aeration strategies with varying air flow rates to optimize fungal growth. Firstly, sterilized moistened air at 8 L/min flows from the bottom through the perforated trays. Secondly, a … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 9 Apr 2024 · 6,453 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,935 accessesView PDFCite
Research NotesOpen accessIn the abstractAdvance in the Diagnostics of Mare Pregnancy and Assessing the Activity of Gonadotropin in Serum of in-Foal MareKaliya Kudaikulovna Yerkekulova, Nuradin Alibayev, Yusupzhan Artykovich Yuldashbayev et al.Research works on the improvement of mare pregnancy diagnostics and evaluation of biological activity of gonadotropic preparation based on the use of enzyme-linked immunosorbent assay were carried out. As the result of diagnostics and evaluation, effective producers as donors for obtaining gonadotropic preparation among the Kazakh population of mares with type “zhabe”, the animal units of 60 days with their … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 7 Mar 2024 · 4,828 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on GlycerolRuixue Wang, Feifei Xin, Yufei Chang et al.More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry of lipid accumulation in oleaginous microorganisms under nitrogen limitation has been extensively studied. As an oleaginous microorganism, Yarrowia lipolytica can produce fatty acid-derived biofuels and biochemicals. However, the response of the enzymes in Y. lipolytica to glycerol remains unclear. Therefore, we aimed … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 2 · 17 Apr 2023 · 6,309 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuantitative Analysis of Hypothalamic Proline-Rich Peptide-1 in Blood Serum of Patients with Malignant TumorsNarine Tumasyan, Silva Abrahamyan, Nune Kocharyan et al.The proline-rich-peptides are a class of neurosecretory hypothalamic bioactive polypeptides derived from cattle and comprise the content of bovine posterior pituitary neurosecretory granules. These peptides are co-validated through a single precursor protein neurophysin-vasopressin-associated glycoprotein. Proline-Rich-Polypeptide-1 (PRP-1) is a 15-amino acid peptide with anti-neurodegenerative, immunoregulatory, hematopoietic, antimicrobial, and antitumor activity. The aim of this study, accordingly, is to elaborate an assay … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 27 Nov 2025 · 2,283 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiosynthesis and Evaluation of Zinc, Copper, and Methionine Feed Supplements Using Saccharomyces cerevisiae on Duck Performance in Minimal Water SystemsHendi Setiyatwan, Muhammad Rifqi IsmirajOptimizing duck performance under minimal water conditions is essential for sustainable poultry farming. Biosynthesizing essential feed supplements like Zinc (Zn), Copper (Cu), and methionine using Saccharomyces cerevisiae presents a promising method to enhance growth and meat quality. This study developed a biosynthesis method for Zn, Cu, and methionine supplements using S. cerevisiae and evaluated their effects on duck performance. In … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 1 · 11 Feb 2025 · 4,405 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Decreasing Temperature on Soil Microbial Activity in a Boreal Shield EcozoneAlistar Moy, Kabwe NkongoloAlthough the focus on warming is imperative to address climate change, addressing the effects of cooling is arguably just as relevant in characterizing the enzyme response to temperature fluctuations. In this study, the response of enzyme activity to diminishing temperatures in the field was evaluated. The targeted sites were located in the City of Greater Sudbury in Northern Ontario, Canada. … …American Journal of Environmental Sciences, Vol. 18, Issue 2 · 17 Apr 2022 · 6,660 accessesView PDFCite
Review ArticleOpen accessIn the abstractOptimization of Red Sorghum (Sorghum bicolor (L.) Moench) Malt Mashing Using Response Surface MethodologyArthur Kapepa Amisi et al.The Response Surface Methodology (RSM) was used to optimize the Released Glucose Equivalent during red sorghum malt mashing. Three parameters were tested: the β-amylase (EC 3.2.1.2) temperature level (min value = 62°C and max value = 63°C, TB), the α-amylase (EC 3.2.1.1) temperature level (min value = 72.5 and max value = 77.5°C, TA) and the duration of each stage … …American Journal of Applied Sciences, Vol. 21, Issue 1 · 28 Nov 2024 · 8,092 accessesView PDFCite