Research ArticleOpen accessEvery word in the titleBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,854 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleProduction of Biodiesel by Enzymatic Transesterification: ReviewA.E. Ghaly, D. Dave, M.S. Brooks, S. BudgeProblem Statement: The research on the production of biodiesel has increased significantly in recent years because of the need for an alternative fuel which endows with biodegradability, low toxicity and renewability. Plant oils, animal fats, microalgal oils and waste products such as animal rendering, fish processing waste and cooking oils have been employed as feedstocks for biodiesel production. In order … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 21,467 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCyclodextrin Glucanotransferase Activity of Microbial Strains Isolated from Yam-Cultivation Soils in Northwestern BeninCyrille Alode Vodounon, Ténor Dias-Mendel Allode et al.β-Cyclodextrin, an industrially important oligosaccharide, is produced by microbial enzymes such as cyclodextrin glucanotransferase (CGTase). This study investigated ways to optimize the enzymatic activity of CGTase from Bacillus cereus, Bacillus pumilus, Bacillus brevis, and Bacillus subtilis strains isolated from soils in north-western Benin. Cyclization activity of CGTase and the influence of pH, temperature, and metal ions were assessed using conventional … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 21 Sep 2026 · 652 accessesView PDFCite
Research ArticleOpen accessIn the keywordsExtraction Optimization and Antibacterial Property of Total Flavonoids from Zizania Latifolia Bracts and the Effect on Chilled Pork PreservationChen Pan, Shuyi Zhu, Na Niu et al.Zizania latifolia isa widely distributed aquatic vegetable, and its bracts are an agriculturalwaste with a large proportion. In this study, Total Flavonoids of Z. latifoliaBracts (TFZB) were extracted with Ultrasound-Assisted Enzymatic Extraction(UAEE) for the first time, and the extraction process was optimized usingResponse Surface Method (RSM), and compared with other methods. Then, theantibacterial activity of TFZB extracted with optimized UAEE … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 15 Sep 2022 · 6,011 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInfluence of Decreasing Temperature on Soil Microbial Activity in a Boreal Shield EcozoneAlistar Moy, Kabwe NkongoloAlthough the focus on warming is imperative to address climate change, addressing the effects of cooling is arguably just as relevant in characterizing the enzyme response to temperature fluctuations. In this study, the response of enzyme activity to diminishing temperatures in the field was evaluated. The targeted sites were located in the City of Greater Sudbury in Northern Ontario, Canada. … …American Journal of Environmental Sciences, Vol. 18, Issue 2 · 17 Apr 2022 · 6,660 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOptimization of Hydrolysis Conditions for the Production of Iron-Binding Peptides from Scad (Decapterus maruadsi) Processing ByproductsWenting Zhang, Yanan Li, Jie Zhang, Guangrong HuangThe scad (Decapterus maruadsi) processing byproduct (SPB) was hydrolyzed by four commercial enzymes, namely, trypsin, flavourzyme, protamex and alcalase, for preparing high Iron-Binding Capacity (IBC) hydrolysate. Alcalase was the best choice for obtaining high IBC hydrolysate from SPB. Response surface methodology using a central-composite design was employed to optimize the enzymatic hydrolysis conditions with alcalase to obtain a maximum hydrolysate … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 17 Oct 2016 · 7,589 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIntegrated Supercritical Fluid Extraction and BioprocessingOwen Catchpole, Stephen Tallon, Peter Dyer et al.Supercritical fluids are increasingly being used and promoted at a laboratory and pilot scale to produce high value, natural bioactives from biologically based raw materials. Supercritical CO2 is overwhelmingly the solvent of choice for these operations, but is largely limited to the processing of dry raw materials and the extraction of low polarity, low molecular weight compounds. The use of … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 9,488 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMeat Spoilage Mechanisms and Preservation Techniques: A Critical ReviewD. Dave, Abdel E. GhalyProblem statement: Extremely perishable meat provides favorable growth condition for various microorganisms. Meat is also very much susceptible to spoilage due to chemical and enzymatic activities. The breakdown of fat, protein and carbohydrates of meat results in the development of off-odors, off-flavor and slim formation which make the meat objectionable for human consumption. It is, therefore, necessary to control meat … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 4 · 2 Nov 2011 · 54,798 accessesView PDFCite