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167 results for “ELISA”

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Research ArticleOpen accessIn the keywordsIncidence of Cystic Echinococcosis in Libya: I. Seroprevalence of Hydatid Disease in Sheep and Goats Naturally Exposed to the Infection in the North Midland RegionMohamed M. Ibrahem, Badereddin B. Annajar, Wafa M. IbrahemHydatid disease is one of the most and serious public health and veterinary problems in Libya and other North African countries. Thirteen rural villages of two main districts bordered to each other at the north midland of the country namely, Misrata which is almost agricultural area and about 200 km east of Tripoli and Sirt which is almost pasture area … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 3 · 28 Oct 2016 · 7,418 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Kit for Multiplexed Detection of Antibodies to Agents of Hemotransmissible InfectionsAlexander Poltavchenko, Anna Ersh, Pavel Filatov et al.The paper presents the results of laboratory trials of method using complex initial testing of antibodies to six agents of hemotransmissible infections: HIV, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The method is based on multiplexed dot immunoassay using plane protein arrays (immunochips) with the use of colloidal gold conjugates and silver development. We describe composition … …OnLine Journal of Biological Sciences, Vol. 16, Issue 3 · 6 Sep 2016 · 7,165 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOccurrence of Feline Immunodeficiency Virus (FIV) and Feline Leukemia (FeLV) in São Luís-MANathálya dos Santos Martins, Ana Paula Sousa Rodrigues, Stefanne Aparecida Gonçalves et al.The aim of this study was to evaluate the occurrence of feline immunodeficiency virus and feline leukemia virus in the city of São Luí­s, Maranhão state (MA), Brazil. A total of 120 blood samples were tested using the Polymerase Chain Reaction (PCR) to detect the proviral DNA, with an amplification of a fragment of 244bp of the FIV's gene gag. … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 3 · 24 Jul 2015 · 8,944 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSerological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic RegionRejane Cristina Ribas-Silva, Taisa Rocha Navasconi, Laís de Souza Braga et al.American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of … …American Journal of Infectious Diseases, Vol. 11, Issue 1 · 23 Jun 2015 · 7,324 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDETECTABLE ANTI-DENGUE VIRUS IGM ANTIBODIES AMONG HEALTHY INDIVIDUALS IN OGBOMOSO, OYO STATE, NIGERIAE. K. Oladipo, C. Amanetu, T. A. Gbadero, J. K. OlokeDengue fever is a zoonosis maintained in nature by mosquitoes transmitting virus between non-human primate species, most of which develop clinically in apparent infection. It has been found to be endemic in Africa and beyond. A survey for Dengue virus IgM antibody was carried out in Ogbomoso (urban and rural areas) using Enzyme Linked Immunosorbent Assay (WKEA Med Supplies Corp, … …American Journal of Infectious Diseases, Vol. 10, Issue 2 · 3 May 2014 · 7,827 accessesView PDFCite
Research ArticleOpen accessIn the keywordsISOLATION AND DETECTION OF HELICOBACTER PYLORI FROM PATIENTS SUFFERING FROM PEPTIC ULCER USING BIOCHEMICAL TESTS AND MOLECULAR TECHNIQUESRuba S. Abu-Sbeih, Azmi D. Hawari, Dhia S. Hassawi, Hala I. Al-DaghistaniHelicobacter pylori is the causative agent of most cases of gastritis. There is no established gold standard for the diagnosis of H. pylori infection. A reliable diagnosis is crucial to confirm that eradication therapy has been successful. Eighty gastric biopsy and blood samples were obtained from fasting Jordanian patients with Esophago-Gastro-Doudenoscopy (EGD). Several diagnosis tests for H. pylori infections were … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Mar 2014 · 8,183 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSCREENING FOR CUCUMBER MOSAIC RESISTANT LINES FROM THE OVULE CULTURE DERIVED DOUBLE HAPLOID CUCUMBERSParichat Plapung, Sirisupaporn Khamsukdee, NutthaPotapohn, Prasartporn SmitamanaCMV is one of the major destructive viruses worldwide and commercial CMV resistant cucumber is very rare. Therefore we aimed to establish the ovule derived resistant lines used for the breeding program. Haploid plants of sixty-eight cucumber lines were successfully obtained by culturing the un pollinated ovaries harvested one day before an thesis on a modified MS medium supplemented with … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Feb 2014 · 7,903 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSoluble CD59-Antigen Levels are Increased in Renal Disease PlasmaOluseyi Adewale VanderpuyeCluster of Differentiation antigen 59 (CD59) is a glycosyl phosphatidylinositol-linked protein that protects cells from damage by lytic terminal complexes of complement. Soluble forms of CD59 in serum have been little investigated in disease states. In the present study, by using a sandwich ELISA, reactivities with antibodies to CD59 were compared for sera and plasma from normal individuals and those … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 6,944 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIgG Subclass Variation of a Monoclonal Antibody Binding to Human Fc-Gamma ReceptorsRekha Patel, Krista K. Johnson, Bruce A. Andrien, Paul P. TamburiniThe importance of human Fc receptors in immune regulation is well known. Their role is critical not only in the recruitment of cellular effector functions but also in regulating the balance in the periphery between autoimmunity and tolerance. Despite their central importance, there is a dearth of literature on controlled numeric comparisons in affinities of antibody subclasses for gamma receptors. … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 8,065 accessesView PDFCite
Research ArticleOpen accessIn the keywordsComparison of enzyme Immunoassay with Immunofluorescence Assay for the Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections in ChildrenOsama Mohammed Saed Abdul-WahabMycoplasma pneumoniae is a frequent cause of acute respiratory infections in both children and adults. Currently, diagnosis of M. pneumoniae infection will based principally on serology and the detection of IgM can provide an early and sensitive diagnosis in children. A variety of commercial immunodiagnostic assays, such as Indirect Enzyme-Linked Immunosorbent (ELISA) assay and Indirect Immunofluorescence (IFA), are now available … …American Journal of Immunology, Vol. 9, Issue 2 · 21 Jun 2013 · 7,412 accessesView PDFCite