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Research ArticleOpen accessIn the abstractGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,944 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiotic and Abiotic Factors of Sperm nDNA Fragmentation in Farm AnimalsBaylar Iolchiev, Natalya Volkova, Pavel Klenovitskiy et al.Infertility and subfertility are among the global challenges of our time. Fertility in livestock farming is not only biologically important, it is also economically important. The aim of this study was to study the effect of these factors on sperm chromatin nucleus and to assess the impact of the fragmentation index degree on bull reproductive performance. The influence of abiotic … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 11 Mar 2021 · 6,714 accessesView PDFCite
Research ArticleOpen accessIn the abstractMechanism Analysis of Artemisinin and Artesunate as Lung Cancer Therapy Agents Through in Silico MethodDidik Harmoko, Vivitri Dewi Prasasty, Adi YulandiSeveral in vitro studies have focused on the ability of artemisinin and its derivatives to suppress lung cancer cells, but there are still many biological processes and mechanisms, such as the interactions between the active compounds (ligands) and receptors that are not well understood and require further research. Drug targeting for lung cancer will be more precise and the inhibition … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 15 Feb 2024 · 6,144 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiomolecular Identification of Bacteria Endophytic From Betel Leaf in Jambi City Forest Park Area as Antibiotic-Producing PotentialArzita, Miranti Sari Fitriani, Nyimas Myrna Elsa Fathia et al.Background and Objective: Muhammad Sabki City Forest Park in Jambi City has three types of betel plants that contain endophytic bacteria. In 2021, six isolates of endophytic bacteria with the potential for producing antibiotics (halo zone above 10 mm) have been isolated, which can kill Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) test bacteria. that are 5 (five) isolates derived … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 15 Feb 2026 · 1,234 accessesView PDFCite
Research ArticleOpen accessIn the abstractAn Effective and Viable DNA Extraction Protocol for FFPE Tissues and its Effect on Downstream Molecular ApplicationSai Sushmitha Kontham, Charles Emmanuel Jebaraj Walter, Manjula Raju et al.Archival Formalin-Fixed Paraffin-Embedded (FFPE) tissues are a readily available source of DNA for retrospective studies including genetic predisposition. FFPE tissues present significant challenges for extraction of pure genomic DNA in adequate amounts due to the harsh fixation conditions and long-term storage associated with samples. The aim of the study is to propose a simple, in-expensive, viable protocol for the extraction … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 24 Mar 2020 · 7,374 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry CultivarsGanies Riza Aristya, Rina Kasiamdari, Muhammad Fauzi Arif et al.Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 19 Mar 2019 · 9,182 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnthelmintic Treatment in Growing Cattle: A Preliminary Study on the Effect of Ricobendazole on the Fecal MicrobiomePablo Rovira, Pablo LorenzoThere is limited information on the impact that anthelmintic treatments exert on the fecal microbiome of growing cattle. This study characterizes the composition and metabolic function of the cattle gut microbiome associated with different levels of parasite Fecal Egg Counts (FEC) and characterizes the fecal microbiome before and after ricobendazole-treatment. The hypothesis was that FEC levels and ricobendazole treatment (8 … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 1 · 31 Mar 2022 · 6,340 accessesView PDFCite
Research ArticleOpen accessIn the abstractDimensionality Reduction using Principal Component Analysis for Cancer Detection based on Microarray Data ClassificationAdiwijaya, Untari N. Wisesty, E. Lisnawati et al.Cancer is one of the most deadly diseases in the world. The International Agency for Research on Cancer (IARC) noted 14.1 million new cancer cases and 8.2 million deaths from cancer in 2012. In the last few years, DNA microarray technology has increasingly been used to analyze and diagnose cancer. Analysis of gene expression data in the form of microarray … …Journal of Computer Science, Vol. 14, Issue 11 · 15 Nov 2018 · 9,930 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Characterization of Kazakh Native Sheep Breeds Using Mitochondrial DNAIlmira Mukhametzharova, Yessenbay Islamov, Saukymbek Shauyenov et al.In this study, we used mitochondrial (mt) DNA to reveal patterns of genetic diversity among six breeds of sheep native to Kazakhstan, including the Chu and Kalchengel types of Kazakh mutton-semifine-wool breed of sheep, Akzhayik, Edilbay, Kazakh fat-rumped semi-course-wool and South Kazakh Merino breeds. Mitochondrial DNA D-loop was studied in 64 samples to determine the genetic diversity of the local … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 7 Sep 2018 · 8,848 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Meat-Derived Lactic Acid Bacteria, Lactobacillus plantarum IIA, Expresses a Functional Parvulin-Like Protein with Unique Structural PropertyCahyo Budiman, Irma Isnafia Arief, Fernandes Opook, Muhammad YusufThe genome sequence of a Lactic Acid Bacterium (LAB) Lactobacillus plantarum IIA contains a single gene encoding a parvulin-like protein (Par-LpIIA). This protein belongs to Peptidyl Prolyl cis-trans Isomerase (PPIase) family proteins that catalyze a slow cis-trans isomerization of cis prolyl bond during protein folding. This study aims to provide molecular and biochemical evidences of the existence of Par-LpIIA in … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 26 Feb 2021 · 7,023 accessesView PDFCite