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89 articles for “Culture Medium”
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Research ArticleOpen accessIn the abstractMining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3Miaomiao Sheng, Huake Jia, Xiaomai Tao et al.Objective of this paper is to excavate the siderophore synthesis gene from Brevibacillus brevis GZDF3 and verify its type and antibacterial effects. The method is using genome mining technology to analyze the siderophore synthesis genes and the phylogenetic tree of each synthesis gene was constructed separately. Iron free medium was utilized to induce the synthesis of siderophore and CAS liquid … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 28 Aug 2018 · 10,611 accessesView PDFCite
Research ArticleOpen accessIn the abstractImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,543 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Enriching Nitrogen and Phosphorus on the Growth of Sargassum Podacanthum Cultured in Potassium-Fortified Inland Saline WaterHa Thi Thu Bui, Trong Quoc Luu, Ravi FotedarPotassium-fortified inland saline water (K+ISW) has shown potential for growing marine species, including seaweed species. The response of a brown seaweed species, Sargassum podacanthum, to nitrogen and phosphorus enrichments were evaluated by culturing the species for 84 days in K+ISW and comparing it with Ocean Water (OW). The culture media were enriched weekly with ammonium chloride and sodium dihydrogen phosphate, … …American Journal of Applied Sciences, Vol. 15, Issue 3 · 6 Apr 2018 · 7,751 accessesView PDFCite
Research ArticleOpen accessIn the abstractNovel Production of Two New Nonpolyenic Antifungal Macrolide Derivatives by Streptomyces Z26 Isolated from Moroccan Rhizospheric SoilAhmed Nafis, Brahim Oubaha, Najoua Elhidar et al.The aim of our study was to screen Moroccan actinomycete isolates able to produce nonpolyenic antifungal metabolites in order to contribute to limiting the problem of fungal infections emergence in particular mycotic diseases and to discover known antifungal families, especially nonpolyenic drugs. 480 isolates were tested against 5clinically pathogenic Candida species. Several methods have been used to study the polyenic … …OnLine Journal of Biological Sciences, Vol. 18, Issue 2 · 8 Mar 2018 · 8,462 accessesView PDFCite
Research ArticleOpen accessIn the abstractMetabolite Profiles of Arsenic Tolerant Plants Regenerated from Stem Calli of Andrographis paniculata (Burm.f.) Nees using HPLC and 1D 1H NMRJ. Vijayakumar, P. Ponmanickam, P. Samuel et al.In vitro culture provide a suitable condition for regeneration of arsenic tolerant plants from stem calli of Andrographis paniculata (Burm.f.) Nees. The regenerated plants could be valuable material applicable to soil remediation. In vitro culture of stem explants on MS basal salts, 3.0% sucrose, 0.8% agar medium fortified with various concentrations of As2O3 (0.0-9.0 µM) along with NAA (3.5 mg … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 27 Oct 2017 · 7,867 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement of in vitro Rooting through Growth Media, Gelling Agents and Activated Charcoal in Lycium chinenseJae Kwang Kim, Sang Un ParkLycium chinense, a traditional Chinese herbal medicine is well known for its medicinal value and composition for centuries in Asia. Plant tissue culture technique has proven as a quick and sustainable ways to regenerate various plants species those are valuable in terms of medicinal as well as ornamental. Here, we investigated the in vitro root regeneration and root growth of … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 26 Jun 2017 · 7,270 accessesView PDFCite
Research NotesOpen accessIn the abstractScreening of Saccharomyces cerevisiae: A Strategy to Enhance Flavour of Jujube LiquorXiaolei Guan, Yaqiong Liu, Yao Lu et al.The main objective of this study was to isolate newly indigenous yeast strains from the fermentation of jujube liquor. 19 isolated yeast strains were obtained by spontaneous solid-state fermentation and flavour characteristics of the fermented jujube juice were also compared from above selected strains. 3 strains (JM-3, JM-13, JM-17) were screened out and identified as Saccharomyces cerevisiae (S. cerevisiae) by … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 3 · 17 Jul 2020 · 6,630 accessesView PDFCite
Research ArticleOpen accessIn the abstractUtilizing of Big Data and Predictive Analytics Capability in Crisis ManagementMohammad Atwah AL-Ma’aitahThis study investigates the impact of Big Data and Predictive Analytics (BDPA) capability on crisis management in the Greater Amman Municipality (GAM), Jordan. Effective utilization of BDPA is considered in the study to depend upon the five dimensions: Tangible resources, technical skills, management skills, organizational learning and data driven culture. Crisis management is classified into the three stages of crisis … …Journal of Computer Science, Vol. 16, Issue 3 · 9 Mar 2020 · 7,868 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free ConditionsFrancesca Abramo, Andrea Pirone, Carla Lenzi et al.Full-thickness canine skin organ culture models could provide an entirely new opportunity for studying wound healing, keratinization disorders and allergic skin diseases, all of which have high prevalence and severe impact on canine quality of life. Here we present a canine organ culture method for the short-term maintenance of full-thickness, adult, canine skin in serum-free medium and investigate the possibility … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 2 · 13 Jun 2016 · 7,383 accessesView PDFCite
Research ArticleOpen accessIn the abstractEconomic Evaluation of Streptococcus Pneumoniae Culture MediaVictor Morais, Norma SuárezStreptococcus pneumoniae, a major bacterial pathogen worldwide, requires a complex medium for culture, which complicates harvesting of antigens for vaccine production. Here we present an economic evaluation of factors affecting the production cost of a chemically defined medium for the scale up of fermentation of S. pneumoniae, compared with the most traditional fermentation media. A basic laboratory-scale experiment of 1 … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 27 May 2016 · 8,379 accessesView PDFCite