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291 articles for “PCR”
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Research ArticleOpen accessIn the abstractMolecular Characterization of Black Bengal and Jamuna Pari Goat Breeds By RAPD MarkersM.A. Rahman, S.M.M. Rahman, M.A. Jalil et al.This experiment was conducted in the Genetics, Breeding and Reproductive Biotechnology Laboratory under Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 14 goat breeds and the extracted DNA was observed by gel electrophoresis. Eight goat specific primers were synthesized by ASM-800 DNA synthesizer and screened in the study and all … …American Journal of Animal and Veterinary Sciences, Vol. 1, Issue 2 · 31 Dec 2006 · 9,584 accessesView PDFCite
Research ArticleOpen accessIn the abstractInflammatory Cytokine Expression Patterns in Rat Skin Exposed to JP-8 Jet FuelRandle M. Gallucci, Bethany M. MickleOne of the main complaints of personnel exposed to JP-8 jet fuel is irritant dermatitis. The purpose of this investigation is to describe the JP-8 induced inflammatory cytokine response in skin over various periods of exposure. JP-8 jet fuel or acetone control (300 μL) was applied to the denuded skin of rats once a day for up to seven days. … …American Journal of Pharmacology and Toxicology, Vol. 1, Issue 3 · 30 Sep 2006 · 7,042 accessesView PDFCite
Research ArticleOpen accessIn the abstractHepatitis, Meningitis and Hydrocephalus Caused by Herpes Simplex Virus Type IMiguel A. Pina, Pedro J. Modrego, F. GalveHerpes simplex virus type is the most frequent cause of sporadic and severe encephalitis in western countries. We report the exceptional case of an immunocompetent man aged 51 and who presented with hepatitis and meningitis by herpes simplex virus. Encephalitis responded to acyclovir but hepatitis did not. The patient developed diffuse hydrocephalus that required the appropriate shunt 20 days after … …American Journal of Infectious Diseases, Vol. 2, Issue 1 · 31 Mar 2006 · 7,155 accessesView PDFCite
Research ArticleOpen accessIn the abstractInterleukin 10 Induces the Maturation of Putative Tolerogenic Monocyte-derived Dendritic Cells as Revealed by GeneArray AnalysisMarianne A. Jakobsen, Bjarne K. MØller, Klaus Rieneck et al.IL-10 is a multifunctional cytokine with known inhibitory effect on DC maturation. In the present study, we show that the presence of IL-10 during the first 6 days of maturation of Mo-DC has severe effects on the phenotype with IL-10 treated Mo-DC retaining a high expression of factors involved in antigen uptake and having low expression of costimulatory factors and … …American Journal of Immunology, Vol. 1, Issue 4 · 31 Dec 2005 · 6,621 accessesView PDFCite
Research ArticleOpen accessIn the abstractSoluble Tumor Necrosis Factor Receptor Type I and Dranulysin as Immunological Markers in Congestive Heart FailureSimone de Lima e Silva Deo, Maria Do Carmo Valente De Crasto, Alda Maria Da-Cruz et al.Previous studies have shown that circulating levels of cytokines are increased in patients with congestive heart failure (CHF), resulting in myocardial depression. In this work, we have determined serum levels of TNF-α and IFN-γ by ELISA, detected sTNFR-I by dot ELISA, as well as TNFα, IL-6, IL-10 and granulysin mRNA in unstimulated peripheral blood mononuclear cells (PBMC) by RT-PCR in … …American Journal of Immunology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,773 accessesView PDFCite
Research ArticleOpen accessIn the abstractHuman Latrophilin-2 is Expressed in the Cytotrophoblast and Syncytiotrophoblast of Placenta and in Endothelial CellsGunda Herberth, Anke Stein, Jens Glienke et al.Latrophilin-2 is a member of the family of adhesion-GPCRs that is characterised by a long N-terminus which contains motifs identified in proteins involved in cell adhesion. We were interested in determining the expression pattern of human latrophilin-2 and to perform a biochemical characterisation of this protein. The expression pattern of latrophilin-2 was analysed in human organs, tissues and cell lines. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 8,053 accessesView PDFCite
Research ArticleOpen accessIn the abstractOxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene ExpressionHidekatsu Yanai, Hiroshi Yoshida, Hironobu Fujiwara et al.Oxidized low-density lipoprotein (LDL) has been shown to be a powerful regulator of gene expression in monocyte-derived macrophage. To determine the effects of oxidized LDL on macrophage gene expression, macrophages incubated with native or oxidized LDL were analyzed by differential display technique. The differentially expressed cDNA (387 bp) fragment by oxidized LDL showed 100% homology to the part of 3’-untranslated … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 7,324 accessesView PDFCite
Research ArticleOpen accessIn the abstractHeparin-PF4 Antibodies in Heparin Induced Thrombocytopenia: Its Relationship with FcgRIIa PolymorphismMeganathan Kannan, Firdos Ahmad, Sarfraz Ahmad et al.We did the pilot study, in an attempt to determine the prevalence of HIT in Indians and determine its relationship, if any, to FcγRIIa polymorphism, the occurrence of heparin-induced thrombocytopenia (HIT) was investigated in 33 Indian patients undergoing cardiovascular surgery (CVS) who received unfractionated heparin (UFH). Platelet counts were performed prior to the initiation of UFH therapy and 5-16 days, … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 7,432 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Time of Flooding Occurrence is Critical for Yield Production in Rice and Vary in a Genotype-Dependent MannerJenjira Mongon, Arom Jantasorn, Prateep Oupkaew et al.Flash floods occur in rainfed lowland and flood-prone areas and have a profound incidence on crop yield, which is tightly linked to worldwide food insecurity. The most flooding-threatened crop is rice. Under this condition, rice undergoes anoxic and post-anoxic shock that affects its growth, development capacity and ultimately causes an important grain yield loss. Therefore, the introduction of submergence-tolerant varieties … …OnLine Journal of Biological Sciences, Vol. 17, Issue 2 · 3 Jun 2017 · 8,093 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtended-Spectrum β-Lactamases Producing Escherichia coli Strains Monitored Over 4 Years in The University Hospital in Košice, SlovakiaViera Lovayová, Lucia Vargová, Mária Nagyová et al.The beta-lactamases with extended spectrum of activity (ESBL) are medically one of the most important group of enzymes. The presented study provides identification and determination of the spectrum of resistance against different and clinically used antimicrobial drugs in the clinical isolates of Escherichia coli. These isolates had their origin in different departments of the University Hospital L. Pasteur in Košice. … …Current Research in Microbiology, Vol. 7, Issue 1 · 16 Jun 2016 · 14,537 accessesView PDFCite