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Research ArticleOpen accessIn the abstractInteraction of a C-terminal Truncated Hepatitis C Virus Core Protein with Plasmid DNA Vaccine Leads to in vitro Assembly of Heterogeneous Virus-like ParticlesNelson Acosta-Rivero, Joanna Poutou, Alexis Mussachio et al.Recently, it has been shown that HCV core proteins (HCcAg) with C-terminal deletions assemble in vitro into virus-like particles (VLPs) in the presence of structured RNA molecules. Results presented in this work showed that a truncated HCcAg variant covering the first 120 aa (HCcAg.120) with a 32 aa N-terminal fusion peptide (6xHistag-XpressTMepitope) interacts with plasmid DNA vaccine. Interestingly, the buoyant … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,754 accessesView PDFCite
Research ArticleOpen accessIn the abstractEssential Role of PKCδ in Apoptosis Induction of Mouse ThymocytesC. Lutz-Nicoladoni, T. Letschka, M. Leitges et al.The family of protein kinases C (PKCs) has been implicated in signal transmission leading to apoptosis induction and/or survival. These effects are cell type and tissue dependent. Numerous studies employing phorbol ester, a pleiotropic PKC activator, strongly implicated PKC in apoptosis induction of thymocytes. However, phorbol esters activate both, the conventional PKCs (PKCα, β, γ) as well as the novel … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 7,135 accessesView PDFCite
Research ArticleOpen accessIn the abstractPhysics Models of Pronuclear Movements in EggsKang Cheng, Changhua ZouBased on published experimental data, Newton's laws, and Coulomb's law, we investigate natural and normal pronuclear movements in wild types of eggs and develop physics models to fit the experimental data quantitatively. The difference between our modeling calculated results and the experimental data is less than 20%. Our models explain why and how pronuclei move in even or in variant … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,743 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Transcript Labeling Techniques and Development of a Membrane-based Parallel Gene Expression AssayMark A. Fisher, Bonnie B. Plikaytis, Thomas M. ShinnickAn inexpensive alternative to micro arrays was developed to examine the expression of small sets of genes in parallel and used to compare direct and indirect transcript labeling methods. Psoralen-biotin direct labeling was ~10-fold less sensitive than reverse transcriptase-biotin-dUTP labeling, but the enzymatic method generated higher levels of background noise in miniarray hybridizations. The reproducibility of hybridization intensities was the … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,868 accessesView PDFCite
Research ArticleOpen accessIn the abstractRAM 1.0 Software for Gaussian-Plume Multiple Source Air Quality SimulationLiren Yu, Paulo I.F. de AlmeidaThis study presents a developed software in environmental engineering, namely RAM 1.0 (RAM: Regulatory Air Model), adopted completely Graphical User Interface (GUI) technique, for operating in various Windows-based microcomputers. RAM 1.0 software consists of the improved RAM Model and RAM Algorithm as well as developed RAM 1.0 Graphical User Interface, respectively. RAM Model takes into account different ambient characteristics, meteorological … …American Journal of Applied Sciences, Vol. 2, Issue 2 · 28 Feb 2005 · 7,458 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnergy and Carbon Flux Coupling: Multi-ecosystem Comparisons Using Artificial Neural NetworkAssefa M. Melesse, Rodney S. HanleyA multi-ecosystems carbon flux simulation from energy fluxes is presented. A new statistical learning technique based on Artificial Neural Network (ANN) back propagation algorithm and multi-layer perceptron architecture was used in the CO2 simulation. Four input layers (net radiation, soil heat flux, sensible and latent heat flux) were used for training (calibration) and testing (verification) of model outputs. The 15-days … …American Journal of Applied Sciences, Vol. 2, Issue 2 · 28 Feb 2005 · 7,347 accessesView PDFCite
Research ArticleOpen accessIn the abstractAn Analysis of the Output and Employment Conversion Matrices of Australia's EconomyAbbas Valadkhani, Tim RobinsonBased on two snapshots taken from the Australian economy, this study quantifies the impacts of final demand aggregates on output and employment in various sectors using the 1989 and 1997 conversion matrices. The sectoral output and employment are linked with final demand deliveries in such a way that one can measure the impacts on changes in each component of aggregate … …American Journal of Applied Sciences, Vol. 2, Issue 2 · 28 Feb 2005 · 6,750 accessesView PDFCite
Research ArticleOpen accessIn the abstractDistribution of Biomicrofacies and Cement Types in the Changxing Formation (Upper Permian), Western Hubei-eastern Sichuan Provinces, South ChinaMutwakil Nafi, Xia WenchenThe Changxing formation is subdivided into lower, middle and upper parts. Four types of microfacies were recognized in the Changxing formation. They include SMF-3, SMF-6, SMF-7 and SMF-19. The Changxing Formation is composed of deep basin margin, platform margin and open restricted platform environments. The lower, middle and upper parts are dominated by deep basin margin facies, platform margin facies … …American Journal of Applied Sciences, Vol. 2, Issue 1 · 31 Jan 2005 · 7,000 accessesView PDFCite