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64 articles for “protein sequence”
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Research ArticleOpen accessIn the abstractSelenium Enriched Peanut Protein Alleviates Alcohol-Induced Liver Damage in Mice by Modulating the Composition of the Gut MicrobiotaLin Gao, Jiawei Yuan, Yuhuan Cheng et al.Alcohol Use Disorder (AUD) is one of leading external causes of mortality. Selenium-enriched Peanut Protein (SePP) is a type of Se compound present in Se-enriched plants. Our previous studies showed that SePP possesses potential therapeutic properties and could alleviate alcohol-induced AML-12 cytotoxicity. It spurred us to determine the underlying mechanisms and roles of SePP on gut microbiota. Here, ICR mice … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 23 Dec 2021 · 6,230 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Meat-Derived Lactic Acid Bacteria, Lactobacillus plantarum IIA, Expresses a Functional Parvulin-Like Protein with Unique Structural PropertyCahyo Budiman, Irma Isnafia Arief, Fernandes Opook, Muhammad YusufThe genome sequence of a Lactic Acid Bacterium (LAB) Lactobacillus plantarum IIA contains a single gene encoding a parvulin-like protein (Par-LpIIA). This protein belongs to Peptidyl Prolyl cis-trans Isomerase (PPIase) family proteins that catalyze a slow cis-trans isomerization of cis prolyl bond during protein folding. This study aims to provide molecular and biochemical evidences of the existence of Par-LpIIA in … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 26 Feb 2021 · 7,023 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization and Differentiation of Proteases Isolated from Different Aspergillus Fungal SpeciesSamia Abd Allah Abdal-Aziz, Safaa M. AliA comparative molecular study of Aperigiullus protease using sequence of protease gene representative 25 different Aspergillus species (Aspergillus fumigatus, Aspergillus oryzae, Aspergillus flavus, Aspergillus novofumigatus, Aspergillus viridinutans, Aspergillus pseudotamarii, Aspergillus clavatus, Aspergillus sojae, Aspergillus fischeri, Aspergillus caelatus, Aspergillus novofumigatus, Aspergillus campestris, Aspergillus costaricaensis, Aspergillus candidus, Aspergillus neoniger, Aspergillus mulundensis, Aspergillus ibericus, Aspergillus niger, Aspergillus piperis, Aspergillus eucalypticola, Aspergillus vadensis, Aspergillus … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 11 Feb 2021 · 6,649 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Analysis of Bifidobacteriaceae in a Fermented Milk and Colostrum Product in Relation to Immune FunctionMarco RuggieroThis study investigates the microbial composition of a commercially available Swiss fermented milk and colostrum product with a history of use in chronic condition management since 2014. The analysis, performed using the Axiom Microbiome Array, identified a high number of targets corresponding to microbial species classified as probiotics belonging to the Bifidobacteriaceae family. The array provided 28 unique targets, each … …American Journal of Immunology, Vol. 19, Issue 1 · 2 Aug 2024 · 9,049 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Endoribonuclease Domain of IRE1 and its Substrate HAC1 are Structurally Linked Components of the Unfolded Protein Response in FungiLuis David Maldonado-BonillaThe unfolded protein response sustains the folding capacity of the endoplasmic reticulum under conditions that increase the activity of the secretory pathway. In fungi, the unfolded protein response exclusively depends on the kinase/endoribonuclease IRE1, which senses the accumulation of unfolded proteins in the endoplasmic reticulum lumen and catalyzes the committed step in the unconventional splicing of HAC1 mRNA that encodes … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 14 Oct 2020 · 6,541 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Polymorphisms at Heat Shock Protein 90 Gene by High Resolution Melting Assays for Potential Heat Tolerance among Nigerian Zebu Cattle BreedsGbolabo Onasanya, George Msalya, Aranganoor Thiruvenkadan et al.Heat Shock Protein (HSP) 90 gene is a member of HSPs sub-family that act as molecular chaperons whenever animals come under thermal stress. The genes fulfill essential roles of providing cellular protection, immune response, protein synthesis, protein folding and unfolding, protection from cellular stress, inhibitory apoptosis and adaptation. This study was designed to analyze polymorphisms of HSP 90 and to … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 1 · 7 Mar 2020 · 7,258 accessesView PDFCite
Research ArticleOpen accessIn the abstractStructural and Substrate Interaction Properties of Alkaline Phosphatase from Paenibacillus sp. PSB04: In-Silico AnalysisHerman Umbau Lindang, Cahyo BudimanA Phosphate-Solubilising Bacterium (PSB) of Paenibacillus sp. PSB04 was previously isolated from the Sabah tropical rainforest in Malaysia. Interestingly, the genome sequence of the PSB04 strain harbored an Alkaline Phosphatase (AP) (EC 3.1.3.1) gene and was hypothesized to have unique structural characteristics. Therefore, this study aims to determine the AP three-Dimensional (3D) model and catalytic mechanism from Paenibacillus sp. PSB04 … …OnLine Journal of Biological Sciences, Vol. 22, Issue 3 · 16 Jul 2022 · 5,910 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Dynamic of Afamin and Alpha-Fetoprotein Expression during Rat Liver DevelopmentIndriyani, Isabella Kurnia Liem, Puspita Eka Wuyung et al.Liver has a high level of Alpha-fetoprotein which is assumed to be important for mammalian development. However, Alpha-fetoprotein knockout studies in mice suggested that despite the absence of Alpha-fetoprotein, mice developed normally; the only abnormality observed was infertility in female mice. There are indications that Afamin, a protein that has a gene sequence located on the same chromosome as Alpha-fetoprotein, … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 5 Feb 2021 · 6,766 accessesView PDFCite
Research ArticleOpen accessIn the abstractEfficient Production of Glycosylated and Non-Glycosylated Mycobacterium tuberculosis Antigen 85B Fused to PVX Coat Protein in Nicotiana benthamiana PlantsPablo A. González, Franco D. Puccio, Alicia M. ZeladaThe development of alternative subunit based-vaccines against tuberculosis is necessary due to variable efficiency and some security concerns of the BCG vaccine. The aim of this work was evaluate the production of the Mycobacterium tuberculosis Ag85B antigen fused to Potato Virus X Coat Protein (PVX-CP) by transient expression in Nicotiana benthamiana for subunit-based tuberculosis vaccine formulation. A codon-optimized M. tuberculosis … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 4 · 13 Nov 2018 · 9,976 accessesView PDFCite
Research ArticleOpen accessIn the abstractSequence Analysis of Scaffold/Matrix Attachment Regions (S/MARs) From Human Embryonic Kidney and Chinese Hamster Ovary CellsNur Shazwani Mohd Pilus, Azrin Ahmad, Nurul Yuziana Mohd YusofBinding of intergenic Scaffold/Matrix Attachment Regions (S/MARs) to nuclear matrix proteins is believed to poise adjacent genes for transcription by forming chromatin loops. Vector constructs containing Scaffold/Matrix Attachment Regions (S/MAR) flanking the gene of interest, therefore, are able to enhance recombinant protein expression in mammalian cells. We compared two methods that are based on buffers containing 2M NaCl and Lithium-3,5-diidosalicylate … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 8 Oct 2018 · 7,630 accessesView PDFCite