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79 articles for “polymerase chain”
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Short CommunicationsOpen accessIn the keywordsMolecular Testing for Respiratory Tract Infections May Have Favorable Impact on Real-world Healthcare CostsAzia Evans, Vijay Singh, Pallavi Upadhyay et al.Molecular testing by Polymerase Chain Reaction (PCR) has improved diagnostic performance to inform appropriate treatment. However, its impact on healthcare utilization and costs related to respiratory tract infections are yet to be studied in detail. The aim of this study is to evaluate the costs of healthcare utilization following traditional culture and molecular PCR diagnostic testing for respiratory tract infections. … …American Journal of Infectious Diseases, Vol. 20, Issue 3 · 15 Jan 2025 · 5,262 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAn Observational Study on the Prevalence of Sexually Transmitted Infections in Cheonan from 2017 to 2021Sun Jung Lee, Tae Su Jang, Jae Kyung KimAlthough the global incidence of sexually transmitted infections is increasing annually, there are few studies in Cheonan that have analyzed the frequency of infection by individual bacterial species by age since 2017. Therefore, in this observational study, we investigated trends in sexually transmitted infections in Cheonan, South Korea, since 2017. From January 2017 to November 2021, 3,297 specimens were obtained … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 17 Apr 2024 · 6,316 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Underestimated Prevalence of Ureaplasma Infections: A Comprehensive Epidemiological Study in South Korea (2018-2020)Jae Eun Choi, Bo Kyeung Jung, Eun Ju Oh, Jae Kyung KimUreaplasma infections are frequently underestimated due to potential inaccuracies in data collection from symptomatic patients. This study aimed to accurately assess the prevalence of Ureaplasma Urealyticum (UU) and Ureaplasma Parvum (UP) infections among outpatients aged 0-70 years in South Korea between 2018 and 2020. A total of 59,381 clinical samples, including swabs and urine specimens, were collected and analyzed using … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 25 Mar 2024 · 6,130 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOccurrence of Feline Immunodeficiency Virus (FIV) and Feline Leukemia (FeLV) in São Luís-MANathálya dos Santos Martins, Ana Paula Sousa Rodrigues, Stefanne Aparecida Gonçalves et al.The aim of this study was to evaluate the occurrence of feline immunodeficiency virus and feline leukemia virus in the city of São Luí­s, Maranhão state (MA), Brazil. A total of 120 blood samples were tested using the Polymerase Chain Reaction (PCR) to detect the proviral DNA, with an amplification of a fragment of 244bp of the FIV's gene gag. … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 3 · 24 Jul 2015 · 8,944 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSerological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic RegionRejane Cristina Ribas-Silva, Taisa Rocha Navasconi, Laís de Souza Braga et al.American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of … …American Journal of Infectious Diseases, Vol. 11, Issue 1 · 23 Jun 2015 · 7,324 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOccurrence of Feline Immunodeficiency Virus and Feline Leukemia Virus Infection in CatsFabiana Alves, Daniela de Souza Rajao, Helen Lima Del Puerto et al.Problem statement: The objective of the present study was to evaluate occurrence of the Feline Immunodeficiency Virus (FIV) and the Feline Leukemia Virus (FeLV) infection in asymptomatic domestic cats in the metropolitan region of Belo Horizonte, Minas Gerais, using the Polymerase Chain Reaction (PCR) for FIV detection and SNAP Combo Plus for FeLV and FIV detection. Approach: Blood samples were … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 3 · 2 Nov 2011 · 8,717 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEarly Diagnosis of Breast Cancer using Molecular, Biochemical and Pathological MarkersSalah El-Din El-Assal, Adel A. El-Tarras, Samir M. Abd-allaProblem statement: Laboratory diagnosis of breast cancer in most of the hospitals has traditionally been performed using cell culture and the direct hormone receptor assay, which are money and time consuming. Approach: This study was performed in order to direct the attention toward increasing the efficiency of early diagnosis in clinical laboratories at the western region of KSA and Egypt … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,056 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMorphological Variability and Races of Fusarium oxysporum f.sp. ciceris Associated with Chickpea (Cicer arietinum) CropsRosa Marina Arvayo-Ortiz, Martin Esqueda, Evelia Acedo-Felix et al.Abstract: Problem statement: Mexico is the third largest producer and exporter of chickpea (Cicer arietinum), with the states of Sinaloa and Sonora accounting for 70 and 20% of Mexico’s production, respectively. The most damaging disease affecting this species is caused by Fusarium oxysporum f.sp. Ciceris (FOC), which causes losses of up to 60% in Sonora. The objective of this study … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 1 · 31 Jan 2011 · 8,853 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetection and Localization of Anti and Pro-apoptotic mRNA Genes in Human Colorectal Cancer Using in situ RT-PCRMohd. Nazil Salleh, Boo Siaw Shi, Wan Khairuzzaman Wan Ramli et al.Problem statement: Recent studies using conventional Polymerase Chain Reaction have shown that anti-apoptotic (Cyclooxygenase-2, COX-2 and Nuclear Factor-κB, NF-κB) and proapoptotic mRNA (Bax and Bad) are involved in the tumorigenesis of colorectal cancer. The aim of this study was to localize the expression of anti and pro-apoptotic mRNA genes using Reverse Transcription in situ Polymerase Chain Reaction (RT in situ … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,441 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHerpetic Viral RetinitisHidetaka NomaHuman Herpes Virus (HHV) is a DNA virus and is the most important viral pathogen causing intraocular inflammation. HHV is classified into types 1-8. Among these types, HHV-1, HHV-2, HHV-3 Varicella Zoster Virus (VZV) and HHV-5 Cytomegalovirus (CMV) are known to cause herpetic viral retinitis, including acute retinal necrosis and CMV retinitis. Herpes viral retinitis can be diagnosed from characteristic … …Current Research in Virology, Vol. 2, Issue 1 · 29 Jul 2013 · 12,788 accessesView PDFCite