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342 results for “in vitro”

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Research ArticleOpen accessEvery word in the titleEffect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling GrowthChieh Wean Choong, Chin Hao Lim, Zherui Xiong, Siew Mee ChoongIn a continuous effort to conserve Phalaenopsis species, the optimization of a previously designed defined medium was carried out to understand the effect of in vitro nitrogen nutrition on Phalaenopsis deliciosa Rchb.f. In order not to alter the nutrient balance, macronutrient level instead of solely Nitrogen (N) was adjusted. Seedlings were obtained from in vitro germination and randomly inoculated on … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 16 Dec 2015 · 7,824 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImmunomodulatory and Antioxidant Properties of Kaurenoic Acid on Macrophages of BALB/c in VitroJuliana Aparecida Macri, Suelen Santos da Silva, Milena Menegazzo Miranda et al.Kaurenoic acid has been displaying anti-inflammatory effect described in different models. However, the per se immunomodulatory effects of kaurenoic acid remain to be investigated. Thus, the immunomodulatory and antioxidant effects of kaurenoic acid were investigated in vitro on peritoneal macrophages from BALB/c mice. Kaurenoic acid induced per se the production of pro-inflammatory cytokines such as TNFα, IL-1β and IFN-γ while … …American Journal of Immunology, Vol. 10, Issue 4 · 16 Feb 2015 · 7,883 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInfluences of Feed Additives on in vitro Volatile Fatty Acid ProductionA. V. Duncan, A. Woldeghebriel, C. Privott et al.This study investigated effects of the feed additives nitrate and fumarate alone or in combination on in vitro Volatile Fatty Acid (VFA) production. Rumen fluid was collected from Holstein-Friesian cattle averaging 650 kg in BW (body weight) offered 11.4 kg d-1 per animal of concentrate diet containing equal amounts of soybean meal, whole cottonseed and ground corn once a day … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 1 · 30 Dec 2014 · 7,227 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOPTIMUM MICRONUTRIENT LEVEL FOR PHALAENOPSIS DELICIOSA ORCHID SEEDLING IN VITRO GROWTHZherui Xiong, Chieh Wean ChoongThere were many researches about the influence of micronutrient concentration toward growth of plants. However, there was no clear statement about the micronutrient level for growth of Phalaenopsis seedlings. Hence it was worth to investigate the optimum level of micronutrient for Phalaenopsis, an endangered orchid species. Using Phalaenopsis deliciosa as the subject, germinated seedlings were grown on defined culture media … …OnLine Journal of Biological Sciences, Vol. 14, Issue 3 · 12 Nov 2014 · 6,629 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEFFECT OF COPPER SULPHATE AND COBALT CHLORIDE ON GROWTH OF THE IN VITRO CULTURE TISSUES FOR DATE PALM (PHOENIX DACTYLIFERA L.) CV. ASHGARAhmed Madi Waheed AL-MayahiThis study was carried out to investigate the effects of copper sulphate and cobalt chloride on propagation of date palm cv. Ashgar in vitro. The rate of callus proliferation was significantly higher in the medium supplemented with 2 µM copper sulphate and 2 µM cobalt chloride together (p<0.05). Addition of copper sulphate and Cobalt chloride to the medium was most … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 1 · 27 Nov 2013 · 8,456 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn Vitro Activity of Tigecycline in the Era of NDM-1Dorairajan Sureshkumar, Ram Gopalakrishnan, M.A. ThirunarayanIn the current era of New Delhi Metallo-betalactamase-1 (NDM-1) infections and a dry antibiotic pipeline, managing infections caused by Multi-Drug Resistant Gram-Negative Bacterial infections (MDR-GNBs) presents a great challenge to physicians in the developing world. Tigecycline, a broad spectrum glycylcycline is a one among the few treatment options against MDR-GNBs. However it has been studied mostly in the western world, … …Current Research in Microbiology, Vol. 3, Issue 2 · 12 Sep 2012 · 7,493 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of Fetal Serum and Follicular Liquor Supplementation on the In Vitro Production of Bovine EmbryosClara Larocca, Yael Filipiak, William Perez, Juan CalvoTo optimize In Vitro Fertilization (IVF) results in bovines by lowering costs, comparing the efficiency of Fetal Calf Serum (FCS) (high cost) with respect to bovine Follicular Fluid (bFF) (low cost) and by quantifying embryo production. Cumulus-Oocyte-Complexes (COC) obtained from a slaughter house, transported at 37°C in saline solution, were classified according to their quality in A, B, C and … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 9 Aug 2012 · 7,839 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn vitro and In vivo Activity of Lactococci Strains against Helicobacter pyloriHassina Guetarni, Ahmed Bensoltane, Djamila Aichour et al.Problem statement: Search for lactic acid bacteria that have in vitro, a significant inhibitory effect against the strains of H. pylori and to determine the inhibitory activity in vivo. Approach: The in vitro inhibitory activity of lactic acid bacteria isolated from milk against strains of H. pylori was determined by the agar diffusion method. Two groups of mice were inoculated … …OnLine Journal of Biological Sciences, Vol. 12, Issue 2 · 15 May 2012 · 7,727 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn Vitro Prevention of Browning in Plantain CultureInnocent Chimereze Onuoha, Chinonye Jemimah Eze, Chibuikem I.N. UnambaProblem statement: The in vitro propagation of plantain (Musa paradisiaca) is still faced with lots of challenges such as blackening or browning of tissues prior to culture due to the oxidation of phenolic compound by polyphenolic oxidase enzyme present in the tissue when excised. Approach: Understanding browning processes in plantain and possible ways of minimizing it during excision of explants … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 15,229 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleSynergistic Effect of Insulin on in vitro Development of Immature Bovine OocytesMojtaba Dashtizad, Abd Wahid Haron, Rosnina Yusoff et al.Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 31 Dec 2010 · 10,433 accessesView PDFCite