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30 articles for “fermentation medium”
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Research ArticleOpen accessIn the abstractImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,543 accessesView PDFCite
Research ArticleOpen accessIn the abstractNovel Production of Two New Nonpolyenic Antifungal Macrolide Derivatives by Streptomyces Z26 Isolated from Moroccan Rhizospheric SoilAhmed Nafis, Brahim Oubaha, Najoua Elhidar et al.The aim of our study was to screen Moroccan actinomycete isolates able to produce nonpolyenic antifungal metabolites in order to contribute to limiting the problem of fungal infections emergence in particular mycotic diseases and to discover known antifungal families, especially nonpolyenic drugs. 480 isolates were tested against 5clinically pathogenic Candida species. Several methods have been used to study the polyenic … …OnLine Journal of Biological Sciences, Vol. 18, Issue 2 · 8 Mar 2018 · 8,462 accessesView PDFCite
Research ArticleOpen accessIn the abstractEconomic Evaluation of Streptococcus Pneumoniae Culture MediaVictor Morais, Norma SuárezStreptococcus pneumoniae, a major bacterial pathogen worldwide, requires a complex medium for culture, which complicates harvesting of antigens for vaccine production. Here we present an economic evaluation of factors affecting the production cost of a chemically defined medium for the scale up of fermentation of S. pneumoniae, compared with the most traditional fermentation media. A basic laboratory-scale experiment of 1 … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 27 May 2016 · 8,379 accessesView PDFCite
Research ArticleOpen accessIn the abstractControl of Subterranean Termite Coptotermes Curvignathus (Isoptera: Rhinotermitidae) by Entomopathogen Metarhizium Anisopliae Var. Anisopliae Cultured in Liquid State FermentationVei-Ting Nyam, Choon-Fah J. Bong, Jie-Hung P. KingSolid state fermentation has been used for mass production of Metarhizium anisopliae many years ago. However, solid state fermentation was time consuming, costly and unable to produce in large amount. We examined the suitability of liquid state fermentation to mass produce M. anisopliae and the effect of culture medium on fungal pathogenicity. Three different liquid medium selected for this study … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 1 · 6 Apr 2015 · 7,536 accessesView PDFCite
Research ArticleOpen accessIn the abstractConversion of Mutton Fat to Cocoa Butter Equivalent by Increasing the Unsaturated Fatty Acids at the Sn-2 Position of Triacylglycerol Through Fermentation by Yarrowia LipolyticaDan Xiong, Huaiyuan Zhang, Yifang Xie et al.Mutton fat has a similar fatty acid profile with Cocoa Butter (CB), except that its degree of unsaturation of Triacylglycerol (TAG) at the Sn-2 position is considerably lower than CB and maybe increased by Sn-2 specific lipase to produce Cocoa Butter Equivalent (CBE), a healthy functional lipid. However, there is no commercially available Sn-2 specific lipase that can be used … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 2 · 4 Mar 2015 · 8,703 accessesView PDFCite
Research ArticleOpen accessIn the abstractScreening of Bacteria for Lactic Acid Production from Whey WaterVethakanraj Helen Shiphrah, Sayanti Sahu, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriLactobacilli have the property of converting lactose and other sugars to lactic acid through fermentation. So whey water, the greenish translucent liquid rich in lactose, vitamins, proteins and mineral salts, obtained as a by-product after the precipitation of cheese can be used as a substrate for Lactobacilli for lactic acid production which otherwise is a serious environmental pollutant when disposed … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 20 Jun 2013 · 9,342 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhanced Production of Extracellular Alkaline Lipase by an Improved Strain of Pseudomonas aeruginosa MTCC 10,055Deepali Bisht, Santosh Kumar Yadav, Nandan Singh DarmwalProblem statement: Lipases are industrially important enzymes having applications in numerous industries. For easy commercialization it is necessary to produce lipases at industrial level which could be achieved by strain improvement and medium formulation. Approach: In the present study strain improvement of Pseudomonas aeruginosa MTCC 10,055 was done by chemical mutagenesis using mutagen 4-nitroquinoline1-oxide for alkaline lipase production. Different fermentation … …American Journal of Applied Sciences, Vol. 9, Issue 2 · 24 Nov 2011 · 8,767 accessesView PDFCite
Research ArticleOpen accessIn the abstractFungal Culture Systems for Production of Antioxidant Phenolics Using Pecan Nut Shells as Sole Carbon SourceMiguel A. Medina, Ruth E. Belmares, Antonio Aguilera-Carbo et al.Problem statement: Many agro-industrial wastes have little or none utilization, when these materials could be a very rich source of several value-added compounds, such as: the pecan nut shells, which contain Antioxidant Phenolic (AP) molecules like tannins. Approach: In this study, a bioprocess for the liberation of AP from Pecan Nut Shells (PNS) was described. A chemical characterization of raw … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,045 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Delaying Sporulation by Addition of Ammonium Sulphate on the Fermentation of Palm Kernal Cake Based Substrate by Aspergillus nigerK. H. Swe, A. R. Alimon, M. RaminProblem statement: Asexual sporulation is a common reproductive mode for a diverse group of fungi and the ultimate limiting factor in fungal biomass production. Approach: Delaying sporulation may increase the biomass in terms of mycelial production and the same time increase production of enzymes. Results: In order to delay the sporulation different levels of ammonium sulphate (10, 12 and 14%) … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 4 · 31 Dec 2009 · 8,402 accessesView PDFCite
Research ArticleOpen accessIn the abstractKinetics of Xylanase Fermentation by Recombinant Escherichia coli DH5α in Shake Flask CultureFarliahati Mohd Rusli, Mohd Shamzi Mohamed, Rosfarizan Mohamad et al.Problem statement: Interest in xylanase enzyme application has markedly increased in pulp and paper processing industries. The switch to xylanase-producing recombinant Escherichia coli DH5α pTP510 is seen here as an economic alternative towards higher productivity and easier downstream purification. Modeling of E. coli DH5α growth and enzyme secretion is thus desired for future optimization in fermentation process. Approach: Kinetics of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,485 accessesView PDFCite