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21 articles for “column chromatography”
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Research ArticleOpen accessIn the abstractMolecular Cloning and Heterologous Expression of Human Interferon Alpha2b GeneI. Made Artika, Yemima Budirahardja, Anna Lucia EkowatiHuman alpha Interferons (hIFNα) have been shown to have antiviral, antiproliferative and immunomodulatory activities. The human interferon alpha2b (hIFNα2b), is one of the human interferon alpha2 sub variants, naturally synthesized as a polypeptide of 188 amino acid residues, the first 23 residues of which represents a signal peptide. In the present study, the hIFNα2b gene was expressed after being fused … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,835 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification and Mode of Action of Antibacterial Components from Melastoma malabathricum Linn LeavesMourouge Saadi Alwash, Nazlina Ibrahim, Wan Yaacob AhmadThe present study aimed to assess the antibacterial activity of the methanol extract of Melastoma Malabathricum Leaves (MMML), active fraction of M. Malabathricum Leaves (ML5) and isolated kaempferol-3-O-(2",6"-di-O-p-trans-coumaroyl)-β-glucopyranoside (Kf) and to describe the preliminary mode of action against Gram-positive and Gram-negative bacteria. The MMML extract was fractionated by Vacuum Liquid Chromatography (VLC) to afford M. malabathricum Leaves fractions (ML1-ML6) of … …American Journal of Infectious Diseases, Vol. 9, Issue 2 · 18 Jun 2013 · 12,621 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Radial and Axial Flow Chromatography for Monoclonal Antibody Downstream Processing at Bench and Pilot ScalesAli Demirci, Frank Leu, F. James BaileyAxial Flow Chromatography (AFC) is widely used for the purification of therapeutic Monoclonal Antibodies (MAbs). However, AFC columns can generate high pressure drops across the resin bed, preventing operation of the column at high flow rates especially at pilot or manufacturing-scales. Radial Flow Chromatography (RFC) was developed to provide lower pressure drops during chromatographic separations. In these studies, small and … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,508 accessesView PDFCite
Research ArticleOpen accessIn the abstractHistorical Changes in Polychlorinated Biphenyls Contaminated SedimentsViet D. Dang, David M. Walters, Cindy M. LeeProblem statement: PCBs contamination continues to pose a health risk to aquatic environments due to their recalcitrance and bioaccumulation. The Sangamo Weston/Twelvemile Creek/Lake Hartwell Superfund Site is an example of such a health risk as a result of PCBs discharged from a former capacitor manufacturing plant. We conducted a study in twelvemile creek (Clemson, SC, USA) to examine the temporal … …American Journal of Environmental Sciences, Vol. 8, Issue 1 · 25 Jan 2012 · 7,077 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioassay Guided Isolation of Antibacterial Compounds from Andrographis paniculataAbubakar Sule, Qamar Uddin Ahmed, Othman Abd. Samah et al.Problem statement: Chronic disease-causing bacteria of medical importance have developed resistance to antibiotics, hence, necessitating distinct and constant need for safe and efficient therapeutic agents. Plants are considered potent candidate for this aim. A way out of reducing antibiotic resistance and adverse effects on host is the employment of antibiotic resistance inhibitors of plant origin. Approach: About 5 kg pulverized … …American Journal of Applied Sciences, Vol. 8, Issue 6 · 13 Jun 2011 · 10,025 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,311 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity ColumnSanhita Roy, Samir Kumar DuttaProblem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 7,095 accessesView PDFCite
Research ArticleOpen accessIn the abstractMass Spectrometry/Mass Spectrometry Study on the Degradation of B-Aflatoxins in Maize with Aqueous Citric AcidA. Méndez-Albores, I. Nicolás-Vázquez, R. Miranda-Ruvalcaba, E. Moreno-MartÍnezDegradation of B-aflatoxins in maize by means of 1N aqueous citric acid was confirmed by the AFLATEST immunoaffinity column method, High Performance Liquid Chromatography (HPLC), tandem mass spectrometry (MS/MS) and computational information. The AFLATEST and HPLC assays showed that 96.7% degradation occurred in maize contaminated with 93 ng g-1 when treated with the aqueous citric acid. Two major products, produced … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 8,049 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol)Chiew Wei Puah, Yuen May Choo, Ah Ngan Ma, Cheng Hock ChuahThis study reports the effect of physical refining on palm vitamin E including α- tocopherol, α-, γ and α-tocotrienols as well as α- tocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with n-hexane/THF/2-propanol (1000:60:4, by vol.) as mobile phase was used. The structure of the α- tocomonoenol was determined using … …American Journal of Applied Sciences, Vol. 4, Issue 6 · 30 Jun 2007 · 8,362 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordDevelopment and Validation of High Performance Liquid Chromatographic Method for the Simultaneous Determination of Ceftazidime and Sulbactam in Spiked Plasma and Combined Dosage form-ZydotamMasoom Raza Siddiqui, Abu Tariq, Manu Chaudhary et al.Problem statement: To develop a sensitive method to determine simultaneously ceftizidime and sulbactam in spiked plasma and combined formulation. Approach: In this study an isocratic High performance liquid chromatographic method with UV detection at 230 nm was described for simultaneous determination of Ceftazidime and sulbactam sodium in plasma and combined dosage form. Chromatographic separation of two drugs was achieved on … …American Journal of Applied Sciences, Vol. 6, Issue 10 · 31 Oct 2009 · 7,672 accessesView PDFCite