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Research ArticleOpen accessIn the abstractEthanol Production by Novel Proline Accumulating Pichia kudriavzevii Mutants Strains Tolerant to High Temperature and Ethanol StressesRika Indri Astuti, Sena Alifianti, Ratu Nabila Maisyitoh et al.Accumulation of osmoprotectant molecule, proline, has been reported to induce a stress tolerance phenotype in yeast cells. In this study, two ethanologenic isolates of yeast, Pichia kudriavzevii (R and T), both capable of using both five and six sugar compounds to produce ethanol, were mutated via ethyl methanesulfonate (EMS) treatment. Prolineaccumulating mutant strains were selected by using proline-analogue (L-azetidine-2-carboxylic acid). … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 11 Sep 2018 · 7,838 accessesView PDFCite
Research ArticleOpen accessIn the abstractDrought Tolerance and Evaluation of Genetic Changes in Rice Mutant LinesAryanti Amsal, Ishak-ishakThe genetic improvement of rice through mutation induction to obtain drought resistance is essential for stable and adequate rice production would enable us to anticipate the rice yield-reducing effects of global warming. The aim of this research was to evaluate the rice mutant lines showing tolerance to drought condition and their genetic relationship using SSR markers. Mutant lines were obtained … …American Journal of Applied Sciences, Vol. 15, Issue 1 · 16 Jan 2018 · 8,547 accessesView PDFCite
InvestigationOpen accessIn the abstractA State of PSI and PSII Photochemistry of Sunflower Yellow-Green Plastome MutantMaksim S. Makarenko, Nikolay V. Kozel, Alexander V. Usatov et al.Chlorophyll deficient mutants are appropriate model objects for a large number of investigations in the field of photosynthesis. In this study, we identified a state of Photosystem I (PSI) and Photosystem II (PSII) photochemistry and light responses of the sunflower yellow-green mutant line - en:chlorina-7, wherein mutations in chloroplast genes have been previously localized. The conducted research revealed low content … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 29 Dec 2016 · 7,458 accessesView PDFCite
Research ArticleOpen accessIn the abstractIs RNA Binding in the Rossmann Fold Essential for GAPDH Intranuclear Functions?Natalia Krynetskaia, Manali Phadke, Evgeny KrynetskiyIn addition to its prominent enzymatic activity, GAPDH is an enigmatic component of multiple unrelated biochemical entities. In this study, we explored a series of mutated GAPDH and fusion EGFP-GAPDH polypeptides and compared nuclear accumulation, intranuclear mobility and RNA binding properties of the wild type and variant GAPDH proteins. Our results revealed that RNA binding to T99I-mutated GAPDH with non-functional … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 14 Jun 2016 · 7,657 accessesView PDFCite
Research ArticleOpen accessIn the abstractChemical Mutagenesis for Improvement of Production of a Biologically Active Extracellular Polymeric Substance by Halomonas xianhensis SUR308Jhuma Biswas, Amal K. PaulA moderately halophilic bacterium, Halomonas xianhensis SUR308 (Genbank Accession No. KJ933394) isolated from solar saltern of Surala, Odisha, India was found to produce significant amount of a biologically active extracellular polymeric substances (EPS). Under optimized cultural conditions, the strain was able to produce 7.05 g L-1 of EPS after 8 days of incubation under shake culture. To enhance the EPS … …Current Research in Microbiology, Vol. 7, Issue 1 · 1 Apr 2016 · 15,918 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Genomics of Domesticated and Wild Sunflower: Complete Chloroplast and Mitochondrial GenomesM. S. Makarenko, A. V. Usatov, N. V. Markin et al.The entire chloroplast and mitochondrial genomes of domesticated and wild type sunflower were sequenced. The comparative analysis of chloroplast genomes revealed 43 variant sites, including 21 polymorphic SSR loci and 22 SNPs. About 14 variant sites were found by collation of mitochondrial DNA (mtDNA), among them 4 SSRs, 8 SNPs and 2 deletions. About 9 SNPs were located in coding … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 29 Feb 2016 · 7,775 accessesView PDFCite
EditorialOpen accessIn the abstractThe Food Additives Nitrite and Nitrate and Microbiological Safety of Food ProductsStefanie Müller-Herbst, Anna Mühlig, Jan Kabisch et al.In Germany, raw sausages are typically produced according to a technology referred to as hurdle technology (Leistner, 2000). The combination of several stressful conditions should successfully inhibit growth and survival of pathogenic bacteria, which can be regularly found in raw pork or beef material (Mataragas et al., 2008; Rhoades et al., 2009). Hurdles applied during raw sausage fermentation are the … …Current Research in Microbiology, Vol. 6, Issue 1 · 29 Jun 2015 · 10,378 accessesView PDFCite
Research ArticleOpen accessIn the abstractProbing the Substrate Specificity and Intersubunit Interactions of Brevundimonas Diminuta Glutaryl Acylase with Site-Directed MutagenesisMichael Eldarov, Anna Lyashenko, Tatyana Sherbakova et al.Glutaryl-7-ACA acylases (GLA) are industrial enzymes widely used for the production of 7-aminocepahlosporanic acid (7-ACA)-the starting compound for manufacturing of semisynthetic cephalosporin antibiotics. Generation of mutant GLA's with increased activity and stability, capability for single-step conversion of cephalosporin C (CPC) directly to 7-ACA is a promising route to improve the current biocatalytic technologies. In this study GLA from Brevundimonas diminuta … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 12 Nov 2014 · 8,160 accessesView PDFCite
Research ArticleOpen accessIn the abstractLEUKOTRIENE C4 SYNTHASE AND LEUKOTRIENE RECEPTOR-1 GENES POLYMORPHISM AMONG ATOPIC ASTHMATIC PATIENTSHeba M. Kadry, Amal H. Atta, Mostafa K. Sultan, Nagwa A. El-BazAsthma is a complex polygenic disease in which Cysteinyl Leukotrienes (Cys-LTs) are a potential risk factors causing airway inflammation and remodeling, which are characteristics of asthma. The polymorphisms in the leukotriene C4 synthase -444A/C and cysteinyl leukotriene receptor1 927 T/C genes has been implicated in susceptibility to asthma. The objective of this study was to analyse two different polymorphisms, LTC4S-444 … …American Journal of Immunology, Vol. 10, Issue 2 · 5 May 2014 · 7,379 accessesView PDFCite
Research ArticleOpen accessIn the abstractDESIGN OF CANDIDA ANTARCTICA LIPASE B THERMOSTABILITY IMPROVEMENT BY INTRODUCING EXTRA DISULFIDE BOND INTO THE ENZYMEUsman Sumo Friend Tambunan, Ahmad Randy, Arli Aditya ParikesitCandida Antarctica Lipase B (CALB) is extensively studied in enzymatic production of biodiesel, pharmaceutical products, detergents and other chemicals. One drawback of using CALB is its relatively low optimum temperature at 313 K (40°C). The objective of this research is to design CALB mutant with improved thermostability by introducing extra disulfide bond. Molecular dynamic simulation was conducted to get better … …OnLine Journal of Biological Sciences, Vol. 14, Issue 2 · 18 Apr 2014 · 9,492 accessesView PDFCite