35 results for “RFLP”

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Research ArticleOpen accessIn the keywordsGenetic Polymorphism Exon 9-11 at the Leptin Gene Receptor in Breeder Hens of Mazandaran Native FowlsHossein Ali Abbasi, Shahabodin Gharavysi, Rohullah AbdullahpourProblem statement: Leptin a 16 kD protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism, we have used a Restriction Fragment Length Polymorphism (RFLP) method. Approach: Blood samples were collected from randomly chosen 100 native fowls breeding station of Mazandaran. The … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 2 · 17 Sep 2011 · 7,700 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsDNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus CattleArash Javanmard, Nader Asadzadeh, Fatollah SarhadiProblem statement: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. Approach: The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 Animals from four breeds from two species of Iranian cattle populations … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,024 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsMolecular Characterization of Pseudomonas aeruginosa UPM P3 from Oil Palm RhizosphereBadri Fariman Azadeh, Sariah MeonProblem statement: Pseudomonas aeruginosa has been used in agriculture as biological agents. It has shown substantial control of a variety of soil-borne plant pathogens including Macrophomina phaseolina, Botrytis cinerea, Rhizoctonia solani, Colletotrichum truncatum, Pythium, Fusarium and others. Species aggregate of Pseudomonas aeruginosa strain UPM P3 was shown to have potential as a biocontrol agent against Ganoderma boninense, the causal agent … …American Journal of Applied Sciences, Vol. 6, Issue 11 · 30 Nov 2009 · 7,802 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsMolecular Characterization of Fusarium Oxysporum F. Sp. Cubense of BananaS. K. Leong, Z. Latiffah, S. BaharuddinProblem statement: Morphological characterization of Fusarium species which emphasize on microscopic and cultural characteristics are not sufficient to characterize Fusarium Oxysporum F. sp. Cubense (FOC) from banana as these characteristics could easily influence by environmental factors. As an alternative molecular methods were used to characterize and to assess genetic variation of FOC from different banana cultivars. Knowledge on the genetic … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 9,267 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsMolecular Analysis of Coagulase Gene Polymorphism in Clinical Isolates of Methicilin Resistant Staphylococcus aureus by Restriction Fragment Length Polymorphism Based GenotypingM. Himabindu, D. Sugapriya Muthamilselvan, Dillip Kumar Bishi, Rama S. VermaProblem statement: To investigate coagulase gene variants through Restriction Fragment Length Polymorphism (RFLP) amongst clinical isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA) collected from Chennai based hospitals. Approach: A total of 85 MRSA clinical isolates collected from various clinical sources were studied for the molecular typing purpose. To perform coagulase gene typing, the repeated units encoding hypervariable regions of the Staphylococcus … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,766 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsAssociation of DNA Polymorphism at the Apolipoprotein B-100 Gene Locus with Plasma Lipid Concentration and Coronary Artery Disease among North IndiansVivek Pratap Singh, V. Ramesh, Sonal Somvanshi et al.The aim of this study was to investigate the association between apolipoprotein B gene polymorphisms and coronary artery disease and lipid levels in north Indians. Two hundred patients of angiographically proven atherosclerotic CAD and two hundred age and sex matched control subjects were included in the study. Serum lipids including cholesterol, triglycerides, HDL, LDL, VLDL and ApoB were analyzed. PCR … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 7,892 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsDifferential Banding Pattern Based Identification of Urinary Tract Infection Causing BacteriaPoulomi Nandy, Md Aftabuddin, Ashoke Ranjan Thakur, Shaon RayChaudhuriUrinary Tract Infection (UTI) affects all age groups, but women are more susceptible than men. These infections are typically caused by E coli, Proteus mirabilis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus fecalis and so on. Since uncultivable microbes are numerically abundant in urine samples, culture independent detection seems to be the method of choice for diagnosing UTI. This study … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 20 Jun 2013 · 8,098 accessesView PDF Cite

Review ArticleOpen accessIn the abstractInsulin-Like Growth Factor 1 Gene and Growth Traits Correlations in Sheep: A Systematic ReviewThabang Sako, Thobela Lousi Tyasi, Jones Ng’ambiAbousoliman, I., Reyer, H., Oster, M., Muráni, E., Mourad, M., Abdel-Salam Rashed, M., Mohamed, I., & Wimmers, K. (2020). Analysis of Candidate Genes for Growth and Milk Performance Traits in the Egyptian Barki Sheep. Animals, 10(2), 197. https://doi.org/10.3390/ani10020197 Al Qasimi, R., Hassan, A., & Khudair, B. Y. (2019). Effect of IGF-1 and GH Genes Polymorphism on Weights and Body Measurements … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 2 · 25 May 2024 · 5,755 accessesView PDF Cite

Research ArticleOpen accessIn the abstractFrequencies of the C1236T, G2677T/A, and C3435T Polymorphisms of the MDR1 Gene and their Haplotypes in UzbekistanS. B. Nurmatova, A. A. Abdurakhimov, O. S. Charishnikova et al.P-glycoprotein (P-gp) is encoded by the MDR1 gene and is involved in the pharmacokinetics of drugs. The best-known MDR1 gene polymorphisms are 1236C>T, 3435C>T, and 2677G>T/A, which are associated with protein folding, changes in mRNA levels, and drug pharmacokinetics. The aim of this study was to identify the frequency of distribution of functional polymorphisms C1236T, C3435T, G2677T/A of the MDR1 … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 5 Dec 2022 · 5,022 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDetermination of Diplotypes Associated with Meat Productivity in Cattle Breeds Common in the Territory of the Republic of KazakhstanAskar Mirzahmetovich Nametov, Indira Saltanovna Beishova, Alena Valentinovna Belaya et al.Technologies for genetic labeling of economically useful traits using polymorphic variants of genes that control the rate of growth and development of animals make it possible to assess the genetic potential of an animal immediately after birth, which significantly reduces time and financial costs. This study aimed to identify paired combinations of the genes growth hormone, growth hormone receptor, and … …OnLine Journal of Biological Sciences, Vol. 22, Issue 3 · 17 Aug 2022 · 6,187 accessesView PDF Cite