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23 articles for “RAPD”
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Research ArticleOpen accessIn the keywordsUsing Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in ThailandKeeravit Petjul, Aphidech Sangdee, Bungorn Thaewnon-ngiwProblem statement: The genetic diversity of Barbodes species in Thailand is presently unavailable. The objectives of this study were to study external morphology and genetic diversity using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) of three Barbodes species; Barbodes altus, B. gonionotus and Barbodes sp. Approach: Two of twelve screened decanucleotide primers (OPA07 and UBC122), were selected for the genetic … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 8,085 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEarly Diagnosis of Breast Cancer using Molecular, Biochemical and Pathological MarkersSalah El-Din El-Assal, Adel A. El-Tarras, Samir M. Abd-allaProblem statement: Laboratory diagnosis of breast cancer in most of the hospitals has traditionally been performed using cell culture and the direct hormone receptor assay, which are money and time consuming. Approach: This study was performed in order to direct the attention toward increasing the efficiency of early diagnosis in clinical laboratories at the western region of KSA and Egypt … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,056 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDrug Susceptibility and Molecular Epidemiology of Pseudomonas aeruginosa Isolated in a Burn UnitHassan Salimi, Parviz Owlia, Bagher Yakhchali, Abdolaziz Rastegar LariProblem statement: Pseudomonas aeruginosa is an opportunistic pathogen that still develops life-threatening infections in patients with immunological system defects, like burn patients, especially in developing countries. Since this bacterium is naturally resistant to many drugs and is able to get resistance to all effective antibiotics, the infection with this organism is particularly problematic condition for patients. This study was design … …American Journal of Infectious Diseases, Vol. 5, Issue 4 · 31 Dec 2009 · 6,992 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Characterization of Opium Poppy (Papaver somniferum) GermplasmHari Shanker Acharya, Vimal SharmaProblem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,096 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHost Plant Mediated Population Variations of Cotton Whitefly Bemisia tabaci Gennadius (Aleyrodidae: Homoptera) Characterized with Random DNA MarkersYasodha Perumal, Murugan Marimuthu, Abida Puthenpeedikal Salim, Balasubramanian PonnusamyProblem statement: Whitefly, Bemisia tabaci is an important sucking pest of field, horticultural and ornamental plants causing feeding injuries besides spreading disease by acting as a vector of Gemini viruses. The polyphagous nature of the pest makes it as a highly complex species. Approach: The influence of host plants utilized by the species on the population differences at molecular level … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 1 · 31 Mar 2009 · 8,211 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGenetic Diversity of Red Chittagong Cattle Using Randomly Amplified Polymorphic DNA MarkersMohammad Masud Rana Mufti, Mst. Parvin Mostari, Gautam Kumar Deb et al.Problem statement: Red Chittagong Cattle (RCC) is a potential genetic resource in Bangladesh having dairy potentialities, high resistance to disease and survive harse environmental condition while require low input supply. This resource is now disappearing due to indiscriminate crossing with other local and exotic cattle. To conserve this resource, find out the genetic diversity of RCC is prime concern. Approach: … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 1 · 17 Mar 2009 · 8,680 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Characterization of Tospovirus Transmitting Thrips Populations from IndiaRang Lal Meena, T. Ramasubramanian, S. Venkatesan, S. MohankumarThe presence of Tospovirus in thrips vector, Scirtothrips dorsalis has been detected for the first time in India using RT- PCR analysis. Similarly RT-PCR analysis with GBNV infected tomato leaves also resulted in the amplification of cDNA corresponding to the N gene (approximately 830 bp) of Coimbatore isolate. Dendrogram constructed on the basis of RAPD similarity matrix revealed that S. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,733 accessesView PDFCite
Research ArticleOpen accessIn the keywordsRandom Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)Lesley Maurice Bilung, Son Radu, Abdul Rani Bahaman et al.Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 8,606 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of the Protein Profile of Cassava Plantlets (Manihot esculenta Crantz.) Resistance to Fusarium Wilt DiseaseEndang Nurcahyani, Hardoko Insan Qudus, Ferina EvlinFusarium wilt disease is still a production constraint in Cassava (Manihot esculenta Crantz.) cultivation. The disease is caused by the fungus Fusarium oxysporum (Fo), which to date has not been cured. It is expected that the use of varieties of cassava that are resistant to Fusarium wilt is an important alternative for disease control. Induced Resistance cassava research on Murashige … …OnLine Journal of Biological Sciences, Vol. 21, Issue 2 · 18 Jun 2021 · 6,777 accessesView PDFCite
Research ArticleOpen accessIn the abstractRANDOMLY AMPLIFIED POLYMORPHIC DNA-A BRIEF REVIEWNandani Kumari, Saroj Kumar ThakurRAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA. The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucleotide sequence polymorphisms in a … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 2 Jan 2014 · 17,258 accessesView PDFCite