14 results for “Masindi L. Mphaphathi”

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Research ArticleOpen accessIn the author namesInvestigation of Oocyte Retrieval Methods and Maturation Media on in vitro Development of Porcine EmbryosKayla M. Honneysett, Masindi L. Mphaphathi, Edward C. Webb, Ayanda MaqhashuThe objectives of the study were to: Compare oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity; compare in vitro maturation (IVM) media (North Carolina State University -NCSU 37; Tissue Culture Medium-TCM 199; and porcine follicular fluid-mpFF) on oocytes polar body extrusion; compare the effect of Electrical Stimulation (ES) and frozen-thawed semen on cleavage rates … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 5 Jan 2022 · 6,600 accessesView PDF Cite

Research ArticleOpen accessIn the author namesSuperovulation Response and Embryo Quality Recovered Following Flushing Nguni Heifers and CowsAyanda Maqhashu, Masindi L. Mphaphathi, Pokgedi J Sebei et al.The aims of the study were to compare super ovulatory response, fertilization rate and evaluate embryo quality recovered from super ovulated Nguni stud cows and heifers. Nguni cows (n = 15) and heifers (n = 10) aged 4-6 and 2 years, respectively, were used as embryo donors. Embryo donors were synchronized for estrus before superovulation. For superovulation donors were administered … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 25 Nov 2021 · 6,431 accessesView PDF Cite

Research ArticleOpen accessIn the author namesStudy the Effect of Extracted Low-Density Lipoprotein from Egg Yolk on the Cryopreservation of Nguni Bull SemenMduduzi M. Tshabalala, Masindi Lottus Mphaphathi, Khathutshedzo A. Nephawe et al.The mechanism on how Egg Yolk (EY) helps sperm to survive the low temperature outside the animal body during cryopreservation and thawing process is not yet known. It is usually believed that the Low-Density Lipoprotein (LDL) is the major component that provides protection. The present study was undertaken to assess the cryo-protective effect of different concentrations (6, 8, 10 and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 13 Sep 2021 · 6,915 accessesView PDF Cite

Research ArticleOpen accessIn the author namesEffect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer SystemMasindi Lottus Mphaphathi, Tshimangadzo Lucky NedambaleThe aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 27 Jan 2021 · 6,591 accessesView PDF Cite