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241 articles for “In Vitro Study”
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Research ArticleOpen accessIn the abstractIn vitro Polyploid Induction in Phalaenopsis Aphrodite by PendimethalinPrasetyorini Djarot, Deden Sukmadjaja, Fadya Annisa Chaesarani, SukonoPhalaenopsis aphrodite is a type of orchid with high ornamental and economic value. However, its development for commercial needs in Indonesia is still hindered by the limited availability of varieties. Therefore, enhancing genetic diversity is necessary, one of which is through polyploid induction. Several previous studies reported that pendimethalin effectively duplicates the chromosome numbers of various plant species. The aim … …OnLine Journal of Biological Sciences, Vol. 26, Issue 3 · 22 Aug 2026 · 854 accessesView PDFCite
Research ArticleOpen accessIn the abstractMarine-Derived Extracts as Economically Sustainable and Ethical Alternatives to Fetal Bovine Serum in HEK293 Cell CultureKian Kenneth Francisco Sanchez, Jennifer Seki-Wong et al.Fetal Bovine Serum (FBS) is a staple supplement in cell culture, providing key growth factors that promote cell health and proliferation. Since 1885, cell culture has been a cornerstone of both basic and applied research, leading to significant discoveries of cell communication and function. However, the use of FBS presents economic and ethical challenges. Millions of bovine fetuses are euthanized … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 7 Aug 2026 · 1,042 accessesView PDFCite
Research ArticleOpen accessIn the abstractOvocidal Effect of Achillea salicifolia and Hedysarum Gmelinii Extracts on Toxocara Cati Eggs in an in Vitro ExperimentLyudmila Lider, Yerlan Suleimen, Gulnur Mamytbekova et al.This study investigated the ovicidal activity of Achillea salicifolia and Hedysarum gmelinii extracts against Toxocara cati eggs under in vitro conditions. Two concentrations (5% and 10%) and four exposure times (0.5, 1, 24, 48 h) were tested in triplicate. The proportion of viable and non-viable eggs, embryo destruction, and shell degeneration were recorded microscopically. Statistical analysis was performed using one-way … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 19 Feb 2026 · 1,620 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro and in Silico Evaluation of Bioactive Compounds from Usnea longissima Ach. as Antimicrobial AgentsOky Kusuma Atni, Erman Munir, Nursahara Pasaribu et al.Lichens, formed from symbiotic association between of fungi with phototrophic organisms, generate a variety of bioactive chemicals having medicinal potential. This research aimed to examine the antibacterial characteristics of Usnea longissima Ach. using both in vitro and in silico methodologies. The effectiveness of lichen methanol extracts against different diseases was evaluated using the disc diffusion technique. Pathogens included gram-positive, gram-negative, … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 10 Feb 2026 · 1,721 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antimicrobial Activity of Modified Lysozyme (Lys 90) and Herbal Extracts Against Multidrug-Resistant Escherichia coliEni Kusumaningtyas, Bagem BR Sembiring, Andriani et al.The emergence of Antimicrobial Resistant (AMR) poses a significant challenge to the poultry farming industry. This study investigated the potential of Lysozyme (Lys), modified Lysozyme (Lys 90), an herbal extract, and their combination (herbs + Lys 90) as alternative antimicrobial agents against Multidrug-Resistant Escherichia coli (MDR-E. coli). Lys 90 was prepared by heating Lys at 90 °C for 20 minutes. … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 1 Dec 2025 · 2,076 accessesView PDFCite
Research ArticleOpen accessIn the abstractGlobal Viewpoint on Cultured Meat: A Bibliometric Assessment from 1952-2023Emrobowansan Monday IdamokoroThis study aimed to describe the global outlook of research publications on cultured meat using bibliometric assessment approach from 1952-2023. A total of 1108 research articles were collected from Scopus data archive with average citations per doc and co-authors per document ratio of 28.74 and 4.46. The results of focus for this study of discourse include author key words, keyword … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 18 Sep 2025 · 4,089 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn silico and in vitro Structural Analysis on the Interaction of Calmodulin and Calmodulin-Binding Motif of FKBP35 from Plasmodium knowlesiSazlinawatie Aladin, Cahyo Budiman et al.FK506-binding protein 35 (FKBP35) is a peptidyl-prolyl cis-trans isomerase found in Plasmodium knowlesi, a zoonotic malaria parasite responsible for concerning cases of malaria infection in East Malaysia. This protein contains a segment known as the calmodulin-binding motif (Pk-CBM), which is predicted to facilitate interaction with calmodulin from P. knowlesi (Pk-CaM). Although this interaction is considered promising for future antimalarial drug … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 10 Mar 2025 · 4,846 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Culture of Oocytes from Dairy Cows' Ovaries for Productivity in the Southeastern Region of KazakhstanDauren Maratovich Bekenov, Batyrkhan Azimkhanovich Buralkhiev, Yusupzhan Artykovich Yuldashbayev et al.This research work aims to study methods for isolating and culturing follicular oocytes from Holstein cows in vitro. The proven complex of technologies for embryo transplantation on an industrial scale will provide a 3-5-fold acceleration of reproduction of highly productive cows. As a result of scientific research, biotechnological methods of accelerated reproduction of dairy cattle have been substantiated and introduced … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 14 Oct 2024 · 4,298 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Kinematics Semen Tolerance to Oxidative StressCyril Mpho PilaneInterpretation of semen kinematic parameters remain as a point of discussion in predicting semen quality following processes like cryopreservation. During cryopreservation, oxidative stress remains a challenge in promoting post-thaw semen quality. Comparison of livestock semen exposed to oxidative stress, in vitro, highlight various aspects of sperm cell movement, like kinematics, to predict their qualities. Sperm cell kinematics includes the measurement … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 13 Sep 2024 · 4,560 accessesView PDFCite
Research ArticleOpen accessIn the abstractAugmentation of Temperatures, Media and Cryoprotectants on in vitro Fertilization of Pig OocytesTumelo Thelma Maduwa, Masindi Lottus Mphaphathi et al.There are numerous challenges to the large-scale production of pig embryos using in vitro procedures. The aim of the present study was to compare the different holding temperatures, in vitro maturation media, and permeating cryoprotectants in the in vitro fertilization of pig oocytes. Ovaries were obtained from the local slaughterhouse, oocytes were retrieved by means of aspiration and slicing techniques … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 29 Aug 2024 · 4,650 accessesView PDFCite