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68 articles for “HPLC”
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Research ArticleOpen accessIn the keywordsCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF "NERIUM OLEANDER" LEAVESLakhmili Siham, Obraim Saida, Taourirte Moha et al.The phenolic products of medicinal plants have a great pharmacological interest. This product gives the powers of medicinal plants. They are the source of several active principles widely used in modern medicine. The use of Nerium oleander in Moroccan traditional medicine is very common. Few studies have focused on the chemical analysis and phenolic compounds of this plant. For this, … …OnLine Journal of Biological Sciences, Vol. 14, Issue 1 · 13 Dec 2013 · 8,285 accessesView PDFCite
Research ArticleOpen accessIn the keywordsFORMULATION AND CLINICAL TESTING OF GLYCERYL TRINITRATE OINTMENT FOR TREATMENT OF ANAL FISSURESEmad Mothana Yousif, Harith Jameel Mahdi, Ali Ibrahem Khaleel, Shathel K. NoamanUntil know surgical intervention considered to be the cornerstone for treatment of anal fissure condition. Regarding all the circumstances that hinder the possibility of accomplishment of surgical operation and the possible post-urgical complications; all that motivates the necessity for a non-surgical procedure for treatment of anal fissure. This study was designed to establish a pharmaceutically active and chemically and physically … …American Journal of Pharmacology and Toxicology, Vol. 7, Issue 3 · 1 Sep 2012 · 8,965 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPhysico-Chemical Characteristics of Date Seed Oil Grown in SudanRehab Salih Mahmoud Abdalla, Alsheikh AlGilani Albasheer, Abdel Rahim Mohamed ELHussein, ELrasheed Ahmed GadkariemProblem statement: Studies were conducted on the physical-chemical properties of Sudanese date seed oil extracted from two date palm cultivars (Phoenix dactylifera L.) ALBarakawi and Alqundeila. The extracted oil from ALBarakawi seeds weighed 6.833% w/w oil, Alqundeila oil extracted weighed 5.064% w/w. Approach: The physical properties of ALBarakawi oil were: viscosity: 17cp, refractive Index: 1.444, density: 0.9116 g cm-3 color: … …American Journal of Applied Sciences, Vol. 9, Issue 7 · 5 May 2012 · 9,500 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Verapamil on Serum Level of Salinomycin in Diabetic RatsHossein Najafzadeh, Said Reza Fatemi, Amir AshnaProblem statement: The aim of present study was evaluation function of P-glycoprotein with or without verapamil in normal and diabetic rats; which the function of P-glycoprotein was indirectly evaluated by detection of serum slainomycin concentration with HPLC method. Approach: This study was carried in 4 groups of rats including normal rats which received salinomycin and verapamil together salinomycin; and diabetic … …American Journal of Applied Sciences, Vol. 8, Issue 9 · 1 Aug 2011 · 6,823 accessesView PDFCite
Research ArticleOpen accessIn the keywordsExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,770 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIdentification and Quantification of Major Carotenoids in Some VegetablesJafar Muhammad EL-QudahAn HPLC study of 6 raw vegetables (Okra, green beans, eggplant zucchini, carrot and tomato) most frequently consumed worldwide was carried out to determine their carotenoid composition. The samples were purchased from supermarket in the city of Boston, USA. Neoxanthin, violaxanthin and lutein were contained in all samples except tomato for neoxanthin, carrot and tomato for violaxanthin and carrot for … …American Journal of Applied Sciences, Vol. 6, Issue 3 · 31 Mar 2009 · 11,131 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetermination of Metoprolol and Felodipine in Binary Mixture Using Chemometric-Assisted Spectrophotometric and High-Performance Liquid Chromatographic-UV MethodsHesham Salem, Ola M. AbdallahFour methods were developed for simultaneous determination of metoprolol and felodipine without previous separation. In the first method both drugs were determined using first derivative UV spectrophotometry, with zero crossing measurement at 222 and 235 nm for metoprolol and felodipine, respectively. The second method depends on first derivative of the ratios spectra by measurements of the amplitudes at 250.1 nm … …American Journal of Applied Sciences, Vol. 4, Issue 9 · 30 Sep 2007 · 8,025 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol)Chiew Wei Puah, Yuen May Choo, Ah Ngan Ma, Cheng Hock ChuahThis study reports the effect of physical refining on palm vitamin E including α- tocopherol, α-, γ and α-tocotrienols as well as α- tocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with n-hexane/THF/2-propanol (1000:60:4, by vol.) as mobile phase was used. The structure of the α- tocomonoenol was determined using … …American Journal of Applied Sciences, Vol. 4, Issue 6 · 30 Jun 2007 · 8,362 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetermination of Diloxanide Furoate and Metronidazole in Binary Mixture Using First Derivative of the Ratio-Spectra and High-Performance Liquid Chromatography-UV MethodsNora H. Al-ShaalanTwo methods are presented for simultaneous determination of diloxanide furoate and metronidazole. The first method depends on first derivative of the ratio-spectra by measurements of the amplitudes at 242.5 and 285.5 nm for diloxanide furoate and 225.5 and 300 nm for metrinidazole. Calibration graphs were established for 2-100 μg mL-1 for diloxanide furoate and 1-50 μg mL-1 for metronidazole. In … …American Journal of Applied Sciences, Vol. 4, Issue 2 · 28 Feb 2007 · 7,713 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHeroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA)Jessica L. Reynolds, Supriya D. Mahajan, Donald Sykes, Madhavan P.N. NairHeroin use is postulated to act as a cofactor in the neuropathogenesis of human immunodeficiency virus (HIV-1) infection. Astrocytes, integral components of the CNS, are reported to be susceptible to HIV-1 infection. Upon activation, astrocytes release a number of immunoregulatory products or modulate the expression of a number of proteins that foster the immunopathogenesis of HIV-1 infection. However, the role … …American Journal of Infectious Diseases, Vol. 2, Issue 2 · 30 Jun 2006 · 7,261 accessesView PDFCite