15 results for “Enzyme Hydrolysis”

Set a search alert

Research ArticleOpen accessIn the abstractIdentification a Novel Raw-Starch-Degrading-α-Amylase from a Tropical Marine BacteriumZeily Nurachman, Alfredo Kono, Ocky Karna Radjasa, Dessy NataliaProblem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-α-amylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifaciens ABBD. The bacterial isolate secreted a α-amylase extracellularly and then the enzyme was partially purified by ammonium sulfate precipitation followed by … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,329 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDesign and Evaluation of Three Pair Primers for Exon 1 Amplification of Hyaluroglucosaminidase-1 GeneUsman Sumo Friend Tambunan, Sylvia Sugito, Arli Aditya ParikesitProblem statement: Hyaluronidase is an enzyme which catalyze hydrolysis of Hyaluronan (HA). Hyaluronan is important in cell migration during embryonic development, cellular proliferation and differentiation and has a structural role in connective tissues. Hyaluronidase deficiency is correlated with mucopolysaccharidosis IX. In human, hyaluronidase is encoded by HYAL1 gene. The mutation study of HYAL1 gene was carried out by many researchers, … …OnLine Journal of Biological Sciences, Vol. 10, Issue 2 · 16 Jun 2010 · 8,702 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCharacterization of Pullulanase Type II from Bacillus cereus H1.5Hii Siew Ling, Tau Chuan Ling, Rosfarizan Mohamad, Arbakariya B. AriffProblem statement: Pullulanase is one of the important enzymes in starch industry. Search for the pullulanase with distinct features, possibly from easily grown bacterium, is of interest for industrial applications Approach: The extracellular pullulanase produced by Bacillus cereus HI.5 was purified by chromatographic method of DEAE-Sepharose, followed by Superdex gel filtration. The enzyme was characterized in terms of the optimal … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 4 · 31 Dec 2009 · 8,808 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordAlkali Pretreatment and Enzymatic Hydrolysis of Cattails from Constructed WetlandsBo Zhang, Abolghasem Shahbazi, Lijun Wang et al.Problem statement: To date, production of liquid fuel, particularly ethanol, has only been economically feasible from food crops that are high in sugar and starch. However, the use of arable land for fuel rather than for food production and the use of a food source for fuel rather than as food have created issues in prices and availability of traditional … …American Journal of Engineering and Applied Sciences, Vol. 3, Issue 2 · 30 Jun 2010 · 9,105 accessesView PDF Cite