32 results for “DNA Detection”

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Research ArticleOpen accessIn the abstractMolecular Detection of Follicle Stimulating Hormone Receptor Polymorphisms and Luteinizing Hormone Gene Variations in Primary and Secondary Iraqi Infertile WomenNoor Basim Naser, Dr. Ezeddin Atia Albayyar, Dr. Susan Abed ZaidanThe current study aimed to determine the role of hormonal disturbances in infertility, moreover assessing variations at the molecular level that could affect this disease. The study examined 90 blood samples, 30 patients with primary infertility, 30 patients with secondary infertility and 30 healthy women as control. FSH and LH were measured using Electrochemiluminescence assay. DNA was isolated for molecular … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 15 Nov 2018 · 7,553 accessesView PDF Cite

Research ArticleOpen accessIn the abstractSensitive and Rapid Detection of Sulfate-Reducing Bacteria in Jet Fuel by Loop-Mediated Isothermal Amplification Combined with Lateral Flow DipstickZezhen Li, Yun Xiong, Peng Zhu, Hailong HuangThe existence of Sulfate-Reducing Bacteria (SRB) is one of the significant reasons for the Microbially Influenced Corrosion (MIC) of jet fuel. Especially for the jet fuel stored by military, since jet fuel is stored in the tank for a long time, some oxygen-consuming bacteria such as Amorphotheca resinae and Bacillus Cohn can consume oxygen and generate organic acids at the … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 11 Apr 2018 · 10,395 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPrevalence of HSV1 and HSV2 among HIV Patients in Northern South Africa as Determined by Real Time PCR in Urine SamplesAmidou Samie, Hlayisani Preclude Mnisi, Mary Noluthando RamantswanaThe present study determined the prevalence of HSV-1 and 2 among HIV patients in Northern South Africa and identified potential risk factors. Urine samples were collected from patients attending different HIV clinics in Limpopo Province. Total genomic DNA was isolated from these samples using GenElute Blood Genomic DNA Kit and real time PCR protocols were used for the detection of … …American Journal of Infectious Diseases, Vol. 13, Issue 1 · 18 Jul 2017 · 7,911 accessesView PDF Cite

HypothesisOpen accessIn the abstractExtending the Repertoire of Structures in DNA NanotechnologyClive DelmonteDNA nanotechnology remains an active area of research and advances have been reviewed recently. DNA nanotechnology seeks to deploy molecules at an atomic level and on a small molecule scale. Other techniques in biophysics and biochemistry do not need to address the issue of the true structure of the nucleic acids at an atomic level but, rather, at a macro-atomic … …Current Research in Nanotechnology, Vol. 6, Issue 1 · 7 Oct 2015 · 13,914 accessesView PDF Cite

EditorialOpen accessIn the abstractNeutrophil Extracellular Traps as Therapeutic Targets for Inflammatory DiseaseRory R. KoenenNeutrophilic granulocytes, or neutrophils are the most abundant circulating white blood cell and constitute a first line of defense against pathogens. They are characterized by a large polymorphic lobular nucleus and the presence of many secretory granules in their cytoplasm. Neutrophils are essential for a functional host defense, as is evidenced by the occurrence of severe infections in individuals with … …American Journal of Pharmacology and Toxicology, Vol. 9, Issue 4 · 25 Feb 2015 · 8,737 accessesView PDF Cite

Research ArticleOpen accessIn the abstractAn Efficient Distributed Bioinformatics Computing System for DNA Sequence Analysis on Encoding SystemMohammad Ibrahim Khan, Chotan SheelThis study provides an effective design of search technique of a distributed bioinformatics computing system for analysis of DNA sequences using OPTSDNA algorithm. This system could be used for disease detection, criminal forensic analysis, gene prediction, genetic system and protein analysis. Different types of distributed algorithms for the search and identification for DNA segments and repeat pattern in a given … …Current Research in Bioinformatics, Vol. 2, Issue 2 · 20 Dec 2013 · 6,931 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDetection of Listeria monocytogenes in Ready-to-Eat Meat ProductsAsma M.M.A. Abdelgadir, Kunwar K. Srivastava, P. Gopal ReddyProblem statement: The objective of this study was to develop a rapid and sensitive method for the detection of Listeria monocytogenes and to screen large number of ready-to-eat (RTE) meat samples. Approach: A total of 250 fresh RTE meat samples of different varieties (chicken, turkey, beef, pork and cold cuts) were purchased from local grocery stores. Results: From the 250 … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 30 Jan 2012 · 7,421 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEvaluation of Nested PCRs targeting the BI and SAG2 Genes For Detection of Toxoplasma gondii Genome in Aqueous Humor From HIV Positive Toxoplasma Retinochoroiditis Patients in a Tertiary Eye HospitalB. Mahalakshmi, K.L. Therese, R. Kirthika et al.Abstract: Problem statement: To evaluate nPCR targeting B1 gene (primer set 1) with 3 other nPCRs targeting B1 gene (primer set 2), 3' end of SAG2 and 5' end of SAG2 gene, for detection of Toxoplasma gondii (T. gondii) genome on AH from HIV positive TRC patients. Approach: DNA extracted from AH of 12 TRC patients and 12 controls (patients … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Dec 2010 · 6,886 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEIAV Effectiveness Detection by Nested PCR Comparing two Different Samples: PBMCs and Bronchoalveolar WashElizangela Maira Dos Santos, Pedro Moreira Couto Motta, Marcos Bryan Heinemann et al.Problem statement: Equine Infectious Anemia (EIA) is a chronic, relapsing infectious disease of horses caused by Equine Infectious Anemia Virus (EIAV). The objective of this experiment was compared EIAV effectiveness detection in Peripheral Blood Mononuclear Cells (PBMCs) and Bronchoalveolar wash (BAW) from naturally infected horses. Approach: Fifty seven DNA samples isolated from PBMCs and BAW of naturally infected animals were … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 7,462 accessesView PDF Cite

Research ArticleOpen accessIn the abstractMolecular Characterization of a Phytoplasma Associated with Coconut Yellow Decline (CYD) in MalaysiaN. Nejat, K. Sijam, S. N.A. Abdullah et al.Problem statement: Molecular methods have been used to detect phytoplasma in Malayan Red Dwarf (MRD) ecotype of coconut for the first time in Malaysia. Detect phytoplasma from coconut showing yellowing symptoms by nested PCR and Classify phytoplasma associated with disease of coconut palms, based on analysis of 16S rRNA gene operon sequences and virtual RFLP. Approach: Twenty MRD symptomatic palms … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 7,919 accessesView PDF Cite