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20 results for “Cryopreservation”
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Research ArticleOpen accessIn the keywordsInfluence of Different Freezing Curves on the Acrosome Integrity of Male Goat Sperm CellsSofia Alexandra Fagundes Lopes, Henrique José Duarte Rosa, António Chaveiro, Fernando Moreira da SilvaThe aim of the present study was to evaluate the effect of three different freezing curves on male goat’s sperm viability and acrosome integrity. Semen was collected on three adult male goats in February (Azores, Portugal, latitude 38°39′N) using an artificial vagina and a female with induced heat. Three collection sessions were spaced of approximately 5 days. The study followed … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 19 Feb 2021 · 6,616 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer SystemMasindi Lottus Mphaphathi, Tshimangadzo Lucky NedambaleThe aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 27 Jan 2021 · 6,591 accessesView PDFCite
Research ArticleOpen accessIn the keywordsKinetic Motilities of Cryopreserved Bull Spermatozoa: Owing to the Effect of Eurycoma longifolia Jack Aqueous ExtractFalah Hasan Baiee, Abd Wahid Haron, Rosnina Haji Yusoff et al.This study was carried out to improve the kinetic motilities of frozen-thawed bull semen diluted with tris-based egg yolk diluent that was supplemented with Eurycoma longifolia jack aqueous extract. A total of 24 ejaculates were obtained from six cross-bred bulls using an electro-ejaculator. The extract of Eurycoma longifolia jack was distributed into three low doses and three high doses; cryopreserved … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 2 · 16 Jun 2017 · 8,635 accessesView PDFCite
Research ArticleOpen accessIn the abstractImproving the Technology for Assessing the Semen of Beige Karakul SheepAigerim Turekyzy Bigarayeva, Nuradin Alibayev, Arkadiy Kanurovich Natyrov et al.The article presents the research results on the effective use of seed products from breeding rams in reproduction for the conservation and targeted propagation of genetic resources of Karakul sheep of rare beige color, which are on the verge of extinction. Selected freshly obtained ejaculates from 6 beige rams of the Karakul breed in the amount of 32 samples, meeting … …OnLine Journal of Biological Sciences, Vol. 25, Issue 1 · 17 Feb 2025 · 4,501 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Kinematics Semen Tolerance to Oxidative StressCyril Mpho PilaneInterpretation of semen kinematic parameters remain as a point of discussion in predicting semen quality following processes like cryopreservation. During cryopreservation, oxidative stress remains a challenge in promoting post-thaw semen quality. Comparison of livestock semen exposed to oxidative stress, in vitro, highlight various aspects of sperm cell movement, like kinematics, to predict their qualities. Sperm cell kinematics includes the measurement … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 13 Sep 2024 · 4,560 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuality of Sperm Simmental Bulls and Success of Artificial Insemination with the Addition of Nanocalcium Phosphate in Tris Aminomethane Egg Yolk Diluent Using Semen Storage Ampoules from Nanocalcium Silicophosphate BiomaterialsEnike Dwi Kusumawati, I Wayan Karyasa, Yogi Pratama Putra et al.Success in Artificial Insemination (AI) is largely determined by the quality of the stored semen, therefore storage devices, especially straws or ampoules, are an important part that cannot be separated from the overall semen cryopreservation technology. Making a prototype of semen storage ampoules from nanocalcium silicophosphate biomaterials is based on the need for straws or ampoules. Semen storage in spermatozoa … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 2 · 28 May 2024 · 5,211 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvidences for the Role of Melatonin as a Protective Additive During Buffalo Semen FreezingMohamed El-Raey, M. R. Badr, Z. M. Rawash, G. M. DarwishTill now, the exact role of melatonin in male infertility hasn’t been fully discovered. Moreover, the intracellular signaling pathways activated by melatonin in buffalo spermatozoa especially on the level of cryopreservation process haven’t been reported. The current study aimed simply to clarify the precise role of this hormone during buffalo semen cryopreservation. Semen samples were obtained randomly from 6 fertile … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 23 Dec 2014 · 8,112 accessesView PDFCite
Research ArticleOpen accessIn the abstractUse of Cooled Bull Semen as a Strategy to Increase the Prenancy Rate in Fixed-Time Artificial Insemination Prgrams-Case ReportAndre Maciel Crespilho, Frederico Ozanam Papa, Marcos de Paula Santos, Manoel Francisco de Sa FilhoSemen cryopreservation is still considered suboptimal due to lower fertility when compared to fresh semen. The reasons for the loss of fertility are various and related to irreversible damage caused to the cells during the freeze-thaw process. An alternative to conventional cryopreservation represents the use of chilled bull semen, preventing the damage associated with freezing, thereby guaranteeing greater sperm viability. … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 4 · 2 Feb 2013 · 8,681 accessesView PDFCite
Research ArticleOpen accessIn the abstractUse of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid DamageClara Gonzalez-Marin, Michael Eduard Kjelland, Rosa Roy et al.Cryopreserved sperm samples from Holstein bulls (n = 20) were examined for bacterial presence and Sperm DNA Fragmentation (SDF) dynamics. SDF was assessed after thawing (T0) and at 4, 24 and 48 h of incubation (37°C) and the rate of SDF (r-SDF), as an estimator of the DNA degradation over time, was calculated. Two groups of bulls were identified based … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 4 · 29 Dec 2012 · 7,430 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Stripping Frequency on Semen Quality of Endangered Caspian Brown Trout, Salmo trutta caspiusSaeed Hajirezaee, Bagher Mojazi Amiri, Ali Reza MirvaghefiProblem statement: Because of dramatic declines in stocks of endangered Caspian brown trout males, Salmo trutta caspius in Caspian Sea, each male brooder is stripped indispensably more than once during the spawning season in other to artificial insemination in hatchery. The aim of the present study was to assay the changes of indicators of semen quality (sperm motility, sperm production, … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 3 · 30 Sep 2009 · 7,478 accessesView PDFCite