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22 results for “16S rDNA”
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Research ArticleOpen accessIn the abstractISOLATION AND CHARACTERIZATION OF RHIZOBIA AND PLANT GROWTH-PROMOTING RHIZOBACTERIA AND THEIR EFFECTS ON GROWTH OF RICE SEEDLINGSK. Z. Tan, O. Radziah, M. S. Halimi et al.Biofertilizer is a relatively safer, environmentally friendly and cost-effective approach as an alternative to reduce chemical fertilizer usage. The selection of bacterial strains with multiple beneficial characteristics are important to maximize the effectiveness on the host plant. Due to aforementioned interest, several Plant Growth-Promoting Rhizobacterial (PGPR) and rhizobial strains were isolated from rice and legume roots, respectively, at four locations … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 15 Apr 2014 · 10,906 accessesView PDFCite
Research ArticleOpen accessIn the abstractRapid Real Time PCR Based Detection of Cell Count in Case of Urinary Tract InfectionPoulomi Nandy, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriMicrobial identification and antimicrobial susceptibility testing methods currently used in clinical microbiology laboratories require at least two to three days because they rely on the growth and isolation of micro-organisms. This long, but necessary, delay has enormous consequences on prophylactic usage of antimicrobial drugs. This study was an attempt to reduce this detection time span. Taq Man Real Time PCR … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 22 Jun 2013 · 8,217 accessesView PDFCite
Research ArticleOpen accessIn the abstractDifferential Banding Pattern Based Identification of Urinary Tract Infection Causing BacteriaPoulomi Nandy, Md Aftabuddin, Ashoke Ranjan Thakur, Shaon RayChaudhuriUrinary Tract Infection (UTI) affects all age groups, but women are more susceptible than men. These infections are typically caused by E coli, Proteus mirabilis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus fecalis and so on. Since uncultivable microbes are numerically abundant in urine samples, culture independent detection seems to be the method of choice for diagnosing UTI. This study … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 20 Jun 2013 · 8,099 accessesView PDFCite
Research ArticleOpen accessIn the abstractDegumming of Raw Silk Fabric with Help of Marine Extracellular ProteaseDas Sumana, Mathummal Sudarshan, Ashoke Ranjan Thakur, Shaon RayChaudhuriProtease secreting microbe was isolated and characterized on the basis of their morphological, biochemical, physiological and 16S rDNA based molecular properties. The extracellular protease was quantified and characterized. Protease was used for different time (4, 8, 12 and 24 h) at different temperature (RT and 37°C) for optimization of the degumming process for raw silk fabric with enzyme dosage (0.2-1 … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 1 · 18 Mar 2013 · 8,504 accessesView PDFCite
Research ArticleOpen accessIn the abstractBACTERIAL ISOLATES OF MARINE COAST AS COMMERCIAL PRODUCER OF PROTEASES. Das, I. Mukherjee, M. Sudarshan et al.The objective of this work was to explore and exploit the extracellular protease secreting marine microbial biodiversity of the eastern coastal region of India. Culture dependent method was applied for isolation of microbes from the marine coast of West Bengal (Digha and Mandarmani) and Andhra Pradesh (Vizag) in India. Six protease secreting isolates were screened using casein hydrolysing property as … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 8 Aug 2012 · 8,799 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Dose-Response of Zinc and Manganese on Siderophores ProductionMehri Ines, Khessairi Amel, Turki Yousra et al.Problem statement: This study was conducted to find and determine whether the siderophores of the four environmental Pseudomonas spp. isolates possess a sequestering activity towards essential transition metals (Zn and Mn) other than iron. Approach: Four fluorescent Pseudomonads isolated from various environments, were characterized analytically (Isoelectric focusing), biologically (pyoverdine-mediated uptake) and genetically (16S rDNA sequencing). By means of spectrophotometric measurements, … …American Journal of Environmental Sciences, Vol. 8, Issue 2 · 10 Mar 2012 · 7,772 accessesView PDFCite
Research ArticleOpen accessIn the abstractLeaf Surface Bacterial Colonization in Response to Brevicoryne brassicae (L.) Infestation of Brassica chinensis L.Shaon Raychaudhuri, Inga Mewis, Christian UlrichsProblem statement: Epiphytic population is an intrinsic part of the leaf surface. The effect of stress like insect infestation is not well understood. The aim of the study was to determine possible effects of insect infestation on bacteria community. Approach: The leaf surface microbial profile of Brassica rapa ssp. chinensis (L.) was investigated using a culture-dependent approach under normal and … …Current Research in Microbiology, Vol. 1, Issue 2 · 31 May 2011 · 7,862 accessesView PDFCite
Research ArticleOpen accessIn the abstractCondoms-As Sources of Extracellular Enzyme Producing MicrobesMadhusmita Mishra, Poulomi Nandy, Sarvani Debsarma et al.Problem statement: Condoms are widely used birth control measures which are made of natural latex or synthetic materials like polyurethane or polyisoprene as matrix with lubricants added to make them ready to use commodities. Absence of sterilization method along with presence of lubricant like petroleum jelly (that are applied on the outer surface), prompted the investigators to look for microbes … …Current Research in Microbiology, Vol. 1, Issue 2 · 31 May 2011 · 8,987 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification a Novel Raw-Starch-Degrading-α-Amylase from a Tropical Marine BacteriumZeily Nurachman, Alfredo Kono, Ocky Karna Radjasa, Dessy NataliaProblem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-α-amylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifaciens ABBD. The bacterial isolate secreted a α-amylase extracellularly and then the enzyme was partially purified by ammonium sulfate precipitation followed by … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,337 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of a Phytoplasma Associated with Coconut Yellow Decline (CYD) in MalaysiaN. Nejat, K. Sijam, S. N.A. Abdullah et al.Problem statement: Molecular methods have been used to detect phytoplasma in Malayan Red Dwarf (MRD) ecotype of coconut for the first time in Malaysia. Detect phytoplasma from coconut showing yellowing symptoms by nested PCR and Classify phytoplasma associated with disease of coconut palms, based on analysis of 16S rRNA gene operon sequences and virtual RFLP. Approach: Twenty MRD symptomatic palms … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 7,923 accessesView PDFCite