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147 articles for “Chromatography”
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Research ArticleOpen accessIn the abstractBio-Guided Study on Melastoma malabathricum Linn Leaves and Elucidation of its Biological ActivitiesMourouge Saadi Alwash, Nazlina Ibrahim, Wan Yaacob AhmadThe present study aimed to isolate and identify constituents with antibacterial activity from the Methanol extract of Melastoma malabathricum leaves (MMML) through bioassay guided fractionation. Furthermore, the study scoped to evaluate the cytotoxic effects of M. malabathricum leaves fractions for possible protective effect of antoxidative constituents present in the fractions. The MMML extract was fractionated by Vacuum Liquid Chromatography (VLC) … …American Journal of Applied Sciences, Vol. 10, Issue 8 · 24 Jul 2013 · 9,519 accessesView PDFCite
Research ArticleOpen accessIn the abstractIncorporation of Omega-3 Fatty Acid and Synthesis of their Metabolites in Muscle Tissue in Mice Fed with Flaxseed and Perilla-Enriched DietSheisa Cyleia Sargi, Flavia Braidotti Stevanato, Marcia Machado de Oliveira Dalalio et al.Polyunsaturated fatty acids omega 3 and omega 6 are believed to have beneficial physiological effects on the human body. The present study aimed to evaluate the incorporation and synthesis of omega-3 fatty acid and their metabolites in muscle tissue in mice fed with a flaxseed and perilla-enriched diet. The animals were fed a diet supplemented with flaxseed and perilla meals … …American Journal of Applied Sciences, Vol. 10, Issue 4 · 17 Apr 2013 · 7,017 accessesView PDFCite
Research ArticleOpen accessIn the abstractIntegrated Supercritical Fluid Extraction and BioprocessingOwen Catchpole, Stephen Tallon, Peter Dyer et al.Supercritical fluids are increasingly being used and promoted at a laboratory and pilot scale to produce high value, natural bioactives from biologically based raw materials. Supercritical CO2 is overwhelmingly the solvent of choice for these operations, but is largely limited to the processing of dry raw materials and the extraction of low polarity, low molecular weight compounds. The use of … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 9,498 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnumeration of Microbes and Gas Production During Denitrification and Nitrogen Fixation Processes in SoilA. E. Ghaly, K. N. MacDonaldDry plant material contains 2-4% nitrogen, making it an essential nutrient for all plants. The nitrogen cycle regulates the pathways which transform nitrogen from a relatively inert dinitrogen gas to forms of organic nitrogen such as proteins and nucleic acids. Denitrification and nitrogen fixation are the two most important processes that remove and add nitrogen to the soil, respectively. The … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 3 · 11 Sep 2012 · 8,622 accessesView PDFCite
Research ArticleOpen accessIn the abstractPlasma Fatty Acid Profile of Gestating Ewes Supplemented with FishmealMamun M. Or-Rashid, Rebecca Fisher, Niel Karrow et al.Problem statement: The very long chain n-3 polyunsaturated fatty acids (>18C) cannot be adequately synthesized by ruminant tissues to meet their requirements; therefore, their concentration in body depends on the supply through feed. It may be possible to improve the essential fatty acid status of ruminant animals, during gestation by manipulating the maternal diet with Fishmeal (FM). The objectives of … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 2 · 9 Jul 2012 · 8,223 accessesView PDFCite
Research ArticleOpen accessIn the abstractGC-MS Analysis and PLS-DA Validation of the Trimethyl Silyl-Derivatization TechniquesKamalrul Azlan Azizan, Syarul Nataqain Baharum, Habtom W. Ressom, Normah Mohd. NoorProblem statement: Comparison and validation analysis of the conventional and power-adjusted Microwave-Assisted (MA) techniques of TMS derivatization using Gas Chromatography-Mass Spectrometry (GC-MS) and Multivariate Analysis (MVA) of Partial Least Squares Analysis (PLS-DA). Further improvement of the conventional technique using vigorous shaking was tested and analyzed. Approach: Cross-validation and response permutation test of PLS-DA and S-plot of Orthogonal Partial Least Squares … …American Journal of Applied Sciences, Vol. 9, Issue 7 · 4 Jun 2012 · 10,325 accessesView PDFCite
Research ArticleOpen accessIn the abstractLevels of Dichlorodiphenyltrichloroethane and its Metabolites in the Blood of Agrochemicals RetailersAnthony J. Hotton, Jeoffrey T. Barminas, Sunday A. OsemeahonProblem statement: In tropical countries like Nigeria, the storage, sales and handling of pesticide by agrochemical retailers in indoor stores located in open markets and residential areas were fraught with problem of undesirable side effect. Serum levels of 1, 1, 1-trichloro-2, 2-di (4-chlorophenyl) ethane (DDT) and its metabolite were measured among agrochemicals retailers in Taraba, Nigeria. Approach: Blood samples from … …American Journal of Environmental Sciences, Vol. 8, Issue 2 · 22 Mar 2012 · 7,459 accessesView PDFCite
Research ArticleOpen accessIn the abstractHistorical Changes in Polychlorinated Biphenyls Contaminated SedimentsViet D. Dang, David M. Walters, Cindy M. LeeProblem statement: PCBs contamination continues to pose a health risk to aquatic environments due to their recalcitrance and bioaccumulation. The Sangamo Weston/Twelvemile Creek/Lake Hartwell Superfund Site is an example of such a health risk as a result of PCBs discharged from a former capacitor manufacturing plant. We conducted a study in twelvemile creek (Clemson, SC, USA) to examine the temporal … …American Journal of Environmental Sciences, Vol. 8, Issue 1 · 25 Jan 2012 · 7,086 accessesView PDFCite
Research ArticleOpen accessIn the abstractAromatic Compound Glucopyranoside from New Species Artocarpus thailandicusWanaree Boonkla, Siripit Pitchuanchom, Puttinan Meepowpan et al.Problem statement: Many Artocarpus plants belonging to the family Moraceae are found in Thailand and have been used as Thai traditional medicines. These plants are known to produce a variety of phenolic glycoside. A. thailandicus is the new species and this prompted us to make further investigations on the occurrence of these types of compounds in the new Artocarpus species. … …American Journal of Applied Sciences, Vol. 8, Issue 11 · 23 Sep 2011 · 8,022 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction, Purification and Characterization of Fish Chymotrypsin: A ReviewLiang Zhou, Suzanne M. Budge, Abdel E. Ghaly et al.Problem statement: Solid fish waste is generated from the unwanted parts of fish including heads, tails, fins, frames, offal (guts, kidney and liver) and skin. It accounts for up to 80% of material from production of surimi, 66% from production of fillet and 27% from production of headed and gutted fish. Currently, fish wastes are disposed off in land-based waste … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 22 Sep 2011 · 10,591 accessesView PDFCite