Effect of Ozonated Water on the Bond Strength of FL Bond II Adhesive to Enamel

The aim of this study was to investigate the potential of ozonated water as an oxidative cleaning solution to improve the bond strength of a bioactive adhesive system to enamel without prior etching. Forty bovine anterior tooth crowns were used and divided into four groups according to the cleaning solution and storage time: AD24 h (cleaning with distilled water, 24 h of storage), AO 24 h (cleaning with ozonized water, 24 h of storage), AD30D (cleaning with distilled water, 30 days of storage) and AO30D (cleaning with ozonized water, 30 days of storage) All groups received the application of the bioactive adhesive system, followed by the fabrication of 2×2 mm bioactive bulk fill flow resin composite cylinders on enamel. After each storage period, the samples were evaluated using micros hear test and fracture analysis. The data was statistically analyzed using JAMOVI software, version 1.2.24. The results indicated that there was no statistically significant difference in most of the tooth thirds and groups evaluated, except in the cervical third after 24 h, where ozonated water showed higher bond strength. This study indicates that ozonated water may enhance the adhesion of bioactive adhesive systems to dental enamel, particularly when specific storage and application conditions are optimized.