Bond Strength of Bioactive vs. Conventional Resin Composites on Dentin Cleaned with Ozonated Water: An In Vitro Study

This study aimed to compare the micro tensile bond strength (μTBS) of bioactive and conventional resin composite to human dentin after the use of ozonated water (AO) as a cavity cleaning solution. A total of 32healthy human third molars were randomly assigned to four groups (n = 8)based on the type of composite and dentin cleaning. The first group received Beautifil Flow Plus F00 with ozonated water (BFP AO). The second group received Opus Bulk Fill with ozonated water (OBF AO). The third group received Beautifil Flow Plus F00 with distilled water (BFP AD), and the fourth group received Opus Bulk Fill with distilled water (OBF AD). The samples were stored in distilled water at 37°C for 24 h before being tested inμ TBS. Failure modes were examined under a stereomicroscope at 40xmagnification and categorized as cohesive-dentin, cohesive-resin, adhesive or mixed. Subsequently, representative specimens from each group were selected for morphological analysis using scanning electron microscopy. Data were initially evaluated for normality using the Shapiro-Wilk test. Given the non-normal distribution, the Kruskal-Wallis test followed by the Dwass-Steel-Critchlow-Fligner post hoc test were employed for statistical analysis. The results showed significantly higher bond strength for the BFPAO group (39.03 MPa; p≤0.05) than the other groups. It can be concluded that ozonated water, when used as a cavity cleaning solution, followed by the application of a bioactive system, significantly enhances the bond strength to dentin.