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6 articles for “trypsin inhibitor”
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Research ArticleOpen accessPhrase in the titlePurification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity ColumnSanhita Roy, Samir Kumar DuttaProblem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 7,095 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffects of Steam Conditioning and Extrusion Temperature on Some Anti-nutritional Factors of Soyabean (Glycine max) for Pet Food ApplicationsB. Purushotham, P.M. Radhakrishna, B.S. SherigaraExtrusion processing of vegetable ingredients such as soyabean (Glycine max) characteristically depends on associating process conditions that influence the product qualities. The process parameters were optimized for extrusion of steam-conditioned material in order to obtain the maximum nutritive value by inactivating the anti-nutritional factors such as urease, trypsin inhibitors and lipase. The processing conditions such as moisture content, temperature and … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Jul 2007 · 9,021 accessesView PDFCite
Research ArticleOpen accessIn the abstractHydroxyl Radical-Scavenging Activity-Guided Extraction of Peptides from Oviductus ranaeJieyang Shen, Yihui Liu, Jiahao Xu et al.Oviductus Ranae (OR) is a traditional Chinese medicinal material that is listed in Chinese Pharmacopeia with proteins the most abundant ingredients. In present work, pepsin/trypsin-based stepwise hydrolysis was applied to extract Antioxidant Peptides from OR (APOR) using hydroxyl radical-scavenging capacity as evaluation index. After single-factor experiment, response surface method coupled with Box-Behnken design were used for optimizing process parameters and … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 18 Oct 2021 · 6,609 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization of Crude Polyphenol Oxidase Activity in Buckwheat SproutsYuedong Song, Yangfang Zhang, Yilin Wang et al.Buckwheat sprouts can not only effectively reduce the trypsin inhibitor in buckwheat grain, but also improve the content of bioactive ingredients such as flavonoids and other effective components. However, Polyphenol Oxidase (PPO) in buckwheat sprouts causes browning of food, which limits the application of buckwheat sprouts in food. In addressing this problem, studying the characteristics of PPO in buckwheat sprouts … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 2 · 25 Jun 2021 · 6,486 accessesView PDFCite
Research ArticleOpen accessIn the abstractBIOCHEMICAL STUDIES ON NIGERIAN MONODORA TENUIFOLIA SEEDEkeanyanwu Raphael Chukwuma, Njoku Obioma UzomaThe nutritive constituents of the seeds of Monodora tenuifolia were analyzed to augment the available information on Monodora tenuifolia research. Blood glucose and lipid profile were investigated on the flavonoid rich fraction of M. tenuifolia in rats. The composition (gkg-1) of alkaloids, cyanogenic glycosides, tannins and flavonoids were 13.3±0.1, 21.2×10-2±0.6, 1.3±0.1, 1.7±0.1 and 11.7±1.1 respectively. The proximate composition (gkg-1) of … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 4 · 30 Sep 2013 · 7,572 accessesView PDFCite