3 articles for “saccharide primer”

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Research ArticleOpen accessIn the keywordsAn Efficient Production of Oligosaccharides by a Reaction using Whole Mammalian Cells as BiocatalystsTomohisa Kato, Rie Mitsumori, Kenichi HatanakaProblem statement: Mammalian cells were used for the production of oligosaccharides by saccharide primer method. However, because cells in culture are used, productivity of oligosaccharides is low. Approach: In saccharide primer method employing cells under culture, glycosylation was performed only by mixing the collected cells in reaction mixtures. Saccharide primer, 12-azidododecyl β-lactoside that mimics lactosylceramide (LacCer), was incubated with various … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,439 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsModification of Artificial Oliogosaccharides in Recombinant Escherichia coli CellsTomohisa Kato, Rie Mitsumori, Kenichi HatanakaArtificial oligosaccharides were modified using recombinant Escherichia coli cells that overexpress sialidase. Based on the principle of the saccharide primer method by using bacterial cells overexpressing enzymes related to oligosaccharide modification. Problem statement: It is very hard to obtain oligosaccharides, because they have complex and diverse structures with different linkage patterns and monosaccharide components. Approach: It had been known that … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,802 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsInhibition of Sphingolipid Biosynthesis in Cultured Cells Enhances the Oligosaccharide Production from Exogenous Artifical SubstrateTomohisa Kato, Maria Carmelita Z. Kasuya, Kenichi HatanakaThe effect of fumonisin B1 which is an inhibitor of ceramide synthesis on saccharide elongation of dodecyl β-lactoside (Lac-C12), which is the analogue of lactosyl ceramide (LacCer), in mouse melanoma B16 cells and African green-monkey kidney (Vero) cells was examined. By adding fumonisin B1 in the culture medium used for glycosylation of Lac-C12, mouse melanoma B16 cells synthesized GM3-type analogue … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 6,669 accessesView PDF Cite