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111 articles for “protein design”
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Research ArticleOpen accessEvery word in the titleIn-silico Rational Protein Engineering and Design Approach to Improve Thermostability of a Haloalkane Dehalogenase EnzymeRaghunath Satpathy, V. Badireenath Konkimalla, Jagnyeswar RathaThermostability of enzymes is a major prerequisite for use in industrial enzymology. There are as such no simple general principles for achieving thermostability in case of enzymes as many factors are required to fulfil for different enzymes. The present study describes computational methods to design thermostable haloalkane dehalogenase enzyme using the crystal structure available Protein Data Bank (PDB ID: 1EDE). … …Current Research in Bioinformatics, Vol. 4, Issue 2 · 13 Oct 2015 · 8,279 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEmerging Trends in Designing Short and Efficient Protein Purification ProtocolsSaurabh Gautam, Joyeeta Mukherjee, Ipsita Roy, Munishwar Nath GuptaProtein purification is carried out in both academic and industrial sectors. Both non-chromatographic approaches (precipitation, crystallization, partitioning) and chromatographic methods are used. When recombinant proteins are over expressed in foreign hosts, inclusion bodies are obtained. Many purification methods can be used to refold proteins. Many strategies allow one to avoid formation of inclusion bodies. The concept of affinity based separation … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 9,007 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Meaning of a Redundant Codon: There is Protein Folding Information in Nucleic Acids in Addition to the Genetic CodeJan C. Biro, Josephine M.K. BiroAll the information necessary for protein folding is supposed to be present in the amino acid sequence. It is still not possible to provide specific ab initio structure predictions by bioinformatical methods. It is suspected that additional folding information is present in protein coding nucleic acid sequences, which is not represented by the known genetic code. Nucleic acid subsequences comprising … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 1 · 31 Mar 2006 · 8,292 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Palm Kernel Cake Based Biosupplement Biscuits on Nutrient Utilization, Growth Performance, and Metabolic Responses in Male Ettawah GoatsTintin Rostini, Suharlina, Achmad Jaelani et al.Palm Kernel Cake (PKC), a major by-product of the palm oil industry, has considerable potential as an alternative feed ingredient for ruminants because of its wide availability, moderate protein content, and economic value. However, its utilization is often constrained by high fiber and lignocellulosic components that may limit nutrient digestibility. This study evaluated the effects of PKC-based biosupplement biscuits as … …American Journal of Animal and Veterinary Sciences, Vol. 21, Issue 3 · 22 Oct 2026 · 312 accessesView PDFCite
Review ArticleOpen accessIn the abstractAdvances in Modern Biotechnological Tools for Sustainable Control of Plant Viral DiseasesNabela Akter, Ankita Saha, Md. Abdullah Al Sabbir, Md. Motaher HossainPlant viruses pose a major threat to global food production, causing yield losses across many staple and horticultural crops. Conventional breeding for resistance is typically slow and constrained by the number of available natural resistance genes. Recent breakthroughs in biotechnology have enabled the development of accurate, flexible, and environmentally friendly technologies to confer virus resistance. This review summarizes exciting developments … …OnLine Journal of Biological Sciences, Vol. 26, Issue 2 · 16 Jul 2026 · 726 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhanced Immunity of Hybrid Grouper (Epinephelus sp.) Against Viral Nervous Necrosis Using a Recombinant Protein Nanovaccine from Chlorella VulgarisUun Yanuhar, Herly Evanuarini, Heru Suryanto et al.Hybrid Grouper (Epinephelus sp.) is an important export commodity in marine aquaculture, yet Viral Nervous Necrosis (VNN) remains a major threat. Nanovaccines are a type of vaccine developed by combining vaccine ingredients with silver nanoparticles and recombinant protein from Chlorella vulgaris. These formulations are designed to effectively boost the body's immune system response to prevent disease infection and destroy pathogenic … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 22 Nov 2025 · 2,154 accessesView PDFCite
Research ArticleOpen accessIn the abstractYield and Nutritional Value of Oats Associated With Vicia in High Andean Conditions of Puno, PeruMarcial Enciso Altamirano, Jimny Nuñez Delgado, Alexandrina Sotelo Méndez et al.The objective of the study was to determine the yield (Kg/ha) and nutritional value of the associated cultivation of oats (Avena sativa) and vicia (Vicia sativa) using different varieties and sowing proportions in high Andean conditions in the Puno region. Three oat varieties were selected: Tayko, Africana and Vilcanota I, and were sown in association with vicia in two proportions: … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 1 Sep 2025 · 5,471 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Leucaena leucocephala Protein Supplement on Nutrient Intake, Milk Yield and Quality, Hematology, Metabolites and Economy Efficiency in Etawah Crossbreed GoatsIdat Galih Permana, Salwa Iffat Zahidah Arif, Fajar Rezki Pambudi et al.Legumes, such as Leucaena leucocephala (LL), have high protein and Rumen degradable Protein (RUP) content, so adding legumes to the diet of dairy goats can meet the protein requirements for milk synthesis. However, the addition of LL alone is insufficient to meet the RUP requirement of dairy goats, thus requiring other protein sources. LL Protein Supplement (LLPS) is a feed … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 23 Aug 2025 · 5,345 accessesView PDFCite
Research ArticleOpen accessIn the abstractAntioxidizing Efficiency of Yogurt Probiotics Peptides Concerning Egg Yolk NutrientsSyifa Silviarahma Nurfadilah, Lovita Adriani, Deny Saefulhadjar et al.Yogurt contains bioactive peptides that serve as antioxidants. These peptides can inhibit the production of free radicals in laying chicken body cells, affecting egg quality. We aimed to evaluate the impact of the antioxidant potential of Water-Soluble Peptide Extract (WSPE) yogurt on the protein, lipid, and cholesterol levels in yolks from chicken eggs. We used a completely randomized design (CRD) … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 1 · 31 Jan 2025 · 6,235 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluating the Impact of Indonesian Herbal Mixture on Recovery and Performance of Bali Cattle After Long-Distance TransportationSri Arnita Abu Tani, Adi Candra, Malik MakmurCattle traveling for an extended period on the road are often experience a decrease in body weight between 10 and 20%. This decrease causes significant losses for farmers, showing the importance of implementing treatment programs for recovery. To overcome the losses, a potential solution could be the use of an Indonesian Herbal Mixture (IHM) comprising various herbal ingredients such as … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 25 Jun 2025 · 4,194 accessesView PDFCite