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12 articles for “cross-linking”
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Research ArticleOpen accessPhrase in the titleUse of Cross-linking to Assess Subunit Interaction of Recombinant Human Coproporphyrinogen OxidaseJason R. Stephenson, Nancy E. Thomas, Jon A. Friesen, Marjorie A. JonesTo provide further evidence for a dimeric form of coproporphyrinogen oxidase reported using the conventional hydrodynamic methods, bifunctional cross-linkers were incubated with purified, recombinant human coproporphyrinogen oxidase to determine subunit interaction in solution. The use of cross-linkers provides an effective way to demonstrate subunit association and allows for assessment of activity upon covalent cross-linking. Following incubation with selected cross-linkers, enzyme … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,594 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleProtective Effects of Covalent Cross-Linking on Proteolysis of Human Coproporphyrinogen Oxidase and Implications for PorphyriaAhmed W. Jafri, Jason R. Stephenson, Justin B. Morgenthaler et al.The effects of covalent cross-linkers on the enzyme, coproporphyrinogen oxidase, had been previously studied but their role in protecting the enzyme from protease cleavage has not been evaluated. Therefore, we examined how the cross-linker bis (sulfosuccinimidyl) suberate (BS3) affects the ability of trypsin to digest purified, wild type recombinant human coproporphyrinogen oxidase and selected mutants. Following incubation, the apparent molecular … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,687 accessesView PDFCite
Research ArticleOpen accessIn the keywordsProperties of Chitosan Nanoparticles Formed Using Sulfate Anions as Crosslinking BridgesMayyas M.A. Al-RemawiProblem statement: The preparation of new simple Chitosan nanoparticle delivery system is very important and could have many applications particularly in pharmaceutical field to be used as a drug delivery system. In this study, the production of robust Chitosan nanoparticles has been developed and characterized by chemical crosslinking using sulfate anions. Approach: Chitosan polymer is considered one of the best … …American Journal of Applied Sciences, Vol. 9, Issue 7 · 28 May 2012 · 9,871 accessesView PDFCite
Research ArticleOpen accessIn the abstractControlled Release Behaviors of Tribenuron Methyl Herbicide and Potassium Nitrate Fertilizer from Natural Polymer Blended Poly(Vinyl Alcohol) Hydrogel MicrospheresFawzi Habeeb Jabrail, Wajeeh Mohammed Jamil, Maher Zeki Elsabee, Kailash Chandra GuptaPolyvinyl Alcohol (PVA) hydrogel microspheres for controlled release of fertilizer (KNO3) and Tribenuron Methyl Herbicide (TBM) were prepared by blending the naturally occurring Alginate (AL) and Pectin (PE). The cross-linking in PVA hydrogel microspheres was carried out by varying the amount of glutaraldehyde from 0.5-1.0 wt%. To control the loading and release properties of PVA hydrogel microspheres, a small amount … …American Journal of Applied Sciences, Vol. 16, Issue 5 · 13 Jul 2019 · 7,673 accessesView PDFCite
Research NotesOpen accessIn the abstractThermal Drying Methods on Coal Liquefaction ConversionMehran HeydariThe sample coal used in this work was lignite containing 25% of moisture and 19% of ash from Canada. Drying of lignite coal had considerable influence on the coal liquefaction conversion. Vacuum dried and nitrogen dried samples provided higher conversion than air dried samples. The air drying showed nearly lower conversion, which probably as a result of oxidation. Air Drying … …American Journal of Engineering and Applied Sciences, Vol. 12, Issue 1 · 12 Mar 2019 · 7,868 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmobilized Whole Cell System with Methyl Parathion-Degrading Activity for Organophosphate Insecticide DetectionChadaporn Pootawee, Witsanu Senbua, Jesdawan WichitwechkarnAs the result of the extensive use and high contamination of organophosphate (OP) insecticides in agricultural products and the environment, especially in developing countries, a simple and convenient means for OP detection and screening was investigated. Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB, gene originating from MP-degrading Burkholderia cepacia and possessing MP-degrading activity were … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 18 Sep 2018 · 7,673 accessesView PDFCite
Research ArticleOpen accessIn the abstractProbing the Substrate Specificity and Intersubunit Interactions of Brevundimonas Diminuta Glutaryl Acylase with Site-Directed MutagenesisMichael Eldarov, Anna Lyashenko, Tatyana Sherbakova et al.Glutaryl-7-ACA acylases (GLA) are industrial enzymes widely used for the production of 7-aminocepahlosporanic acid (7-ACA)-the starting compound for manufacturing of semisynthetic cephalosporin antibiotics. Generation of mutant GLA's with increased activity and stability, capability for single-step conversion of cephalosporin C (CPC) directly to 7-ACA is a promising route to improve the current biocatalytic technologies. In this study GLA from Brevundimonas diminuta … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 12 Nov 2014 · 8,160 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Proteases on Meltability and Stretchability of Nabulsi CheeseKhaled Abu-Alruz, Ayman Suliman Mazahreh, Jihad M. Quasem et al.Problem statement: Boiled white brined cheese (Nabulsi cheese) is the mostly consumed cheese in Jordan; this cheese should show meltability and high stretchability in order to fit in the production of high quality Kunafa and other popular local sweets and pastries. However, these characteristics are rarely available when usual processing and preservation methods were used. Approach: This study was based … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 3 · 30 Sep 2009 · 8,830 accessesView PDFCite
Research ArticleOpen accessIn the abstractMeltability and Stretchability of White Brined Cheese: Effect of Emulsifier SaltsKhaled Abu-Alruz, Ayman Suliman Mazahreh, Ali F. Al-Shawabkeh et al.Problem statement: This study was based on the hypothesis that by adding low concentrations of emulsifier salts, may specifically act on the cross linking bonds of the protein matrix, to the original brine (storage medium) it would be possible to induce meltability and stretchability in white brined cheese. Approach: A new apparatus for measuring the actual stretchability was designed and … …American Journal of Applied Sciences, Vol. 6, Issue 8 · 31 Aug 2009 · 7,093 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of adjusting PH on Stretchability and Meltability to White Brined Nabulsi CheeseAyman Suliman Mazahreh, Jihad M. Quasem, Ali Faleh Al-Shawabkeh, Ibrahim Abdullah AfanehProblem statement: Boiled white brined (Nabulsi cheese) is the mostly consumed in Jordan; this cheese should show meltability and high stretchability in order to fit in the production of high quality Kunafa and other popular local sweets and pastries.The most outstanding characteristic of Nabulsi cheese is the long keeping ability (more than one year) without cooling, since it is preserved … …American Journal of Applied Sciences, Vol. 6, Issue 4 · 3 Apr 2009 · 7,786 accessesView PDFCite