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Research ArticleOpen accessSeparation of Guanine and Hypoxanthine with Some Ionic Liquids in RP-HPLCJ. Zheng, K. H. RowIn this paper, guanine and hypoxanthine were separated with four different ionic liquids as additives for the mobile phase using reversed phase high performance liquid chromatography (RP-HPLC). The ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) and 1-octyl-3-methylimidazolium methylsulfate ([OMIm][MS]) were used. Guanine and hypoxanthine couldn’t be separated with many different kinds of unadjusted mobile phase, such … …American Journal of Applied Sciences, Vol. 3, Issue 12 · 31 Dec 2006 · 6,892 accessesView PDFCite