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40 articles for “Western blot”
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Research ArticleOpen accessPhrase in the titleAn Alternative to the Traditional Western Blot For Recombinant His-tagged ProteinsJustin B. Morgenthaler, Jason R. Stephenson, Jon A. Friesen, Marjorie A. JonesA rapid, inexpensive and sensitive alternative to the traditional Western Blot is presented here. This method is very useful for detection of recombinant 6-his tagged proteins. …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,846 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSDS-PAGE Protein Pattern and Antigenicity Cross Reaction of Human SchistosomesNahed A.A. EL-Ossily, Doaa A. Yones, Mohamed El-Salahy M.M. Monib et al.Schistosomiasis is one of the most important neglected tropical diseases. Its control depends on treatment with the available drug praziquantel. No vaccine exists despite the intense search for molecular candidates and adjuvant formulations over the last three decades. The present study aimed to compare the antigenic protein structures of Schistosoma mansoni, S. haematobium, Fasciola. hepatica and Echinococus granulosus hydatid cyst … …American Journal of Infectious Diseases, Vol. 12, Issue 1 · 10 Feb 2016 · 7,354 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAntigenic and Immunogenic Components of Haemonchus longistipes Identified by Western ImmunoblotingE. M. El Hassan, Sabry M. El-BahrThe present study aimed to examine the ways that the Haemonchus longistipes components interact with the host by investigating the components of the parasite which acted as antigens during infection and those who are capable of inducing immune response when administered as immunogens. Adult H. longistipes worms (300 females and 200 males) were obtained from the abomasums of naturally infected … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 3 · 20 Sep 2012 · 7,498 accessesView PDFCite
Research ArticleOpen accessIn the keywordsNa,K-ATPase α Subunit-like Immunoreactive Proteins in Human Body FluidsJeng-Hsiung F. PengNa,K-ATPase was recently considered a target for breast cancer treatment since its potent inhibitors ouabain and related digitalis compounds that were reported circulating in body fluids before, were found to possess potent anti-breast cancer activity. Based on this finding, it is hypothesized that Na,K-ATPase derivatives could be circulating in the body and potential candidate biomarkers for early disease detection. Western … …American Journal of Immunology, Vol. 2, Issue 1 · 31 Mar 2006 · 6,892 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPurification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity ColumnSanhita Roy, Samir Kumar DuttaProblem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 7,095 accessesView PDFCite
Research ArticleOpen accessIn the keywordsELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) SpeciesPhumzile Mkhize, Phetole MangenaFusarium contains the most toxigenic and important fungal pathogens, producing a wide range of mycotoxins that include the more toxic secondary metabolites called fumonisins. This study analyzed the presence of common exo- and mycelium-soluble antigens, purified proteins using reducing SDS-PAGE and the expression of antibodies against different fungal pathogens. The results showed the presence of common antigens between pathogens and … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 18 Nov 2020 · 6,601 accessesView PDFCite
Research ArticleOpen accessIn the abstractCloning and Expression of SCAMP3 in Transgenic Silkworm (Bombyx mori)Selly S. Rajagukguk, Sabar Pambudi, Astari Dwiranti et al.SCAMP3 (Secretory Carrier Membrane Protein 3) is a crucial component of vesicular trafficking pathways and plays important roles in endocytosis and protein recycling. Previous studies demonstrate SCAMP3 dysregulation in various cancers, suggesting potential applications as a biomarker and therapeutic target. Understanding SCAMP3 expression and post-translational processing provides insights into its biological functions and potential applications in cancer diagnostics and therapy. … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 16 Feb 2026 · 1,521 accessesView PDFCite
Research ArticleOpen accessIn the abstractClinical Significance of Precise Hyperthermic Intraperitoneal Chemotherapy Up-regulation RP11-356I2.2 Restraining the Occurrence and Development of Gastric CancerYu Yan, Feng Yanlin, Feng Jinxin et al.In order to study the therapeutic effect and possible mechanism of Precise Hyperthermic Intraperitoneal Chemotherapy (HIPEC) on gastric cancer, the expression of RP11-356I2.2 in gastric cancer cell lines and tissues was detected by real-time fluorescence quantitative PCR (RT-PCR). Chi-square test was used to analyze the correlation between the expression and clinic pathological parameters. Western blotting was used to detect the … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 4 Jun 2020 · 6,487 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in Pichia pastoris KM71Popi Asri Kurniatin, Fifi Fitriyah Masduki, Neni Nurainy et al.A spacer peptide (Glu-Ala repeats) connects N-terminal of a polypeptide precursor to the signal peptide of α-mating factor (α-MF) from Saccharomyces cerevisiae. During the polypeptide precursor secretion, the signal peptide will be first cleaved by signal peptidase in the endoplasmic reticulum, followed by Kex2 endopeptidase and finally the spacer peptide is removed by dipeptidyl aminopeptidase in the Golgi apparatus. In … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 31 Oct 2019 · 9,026 accessesView PDFCite
Research ArticleOpen accessIn the abstractDihydroartemisinin Attenuates HBV-Induced Proliferation and Migration in LO2 cells via EMT Pathway ModulationYingde Nong, Caifang Ma, Wei Huang et al.Dihydroartemisinin (DHA), a derivative of artemisinin, possesses well-documented anti-inflammatory properties. This study investigated the inhibitory effects of DHA on hepatitis B virus (HBV)-induced cellular alterations in LO2 cells and elucidated underlying molecular mechanisms. Serum interleukin-6 (IL-6) levels were measured in HBV patients and analyzed for correlations with HBsAg, anti-HBs, and HBeAg. In vitro, LO2 cells were infected with clinical HBV … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 3 · 8 Jan 2026 · 2,071 accessesView PDFCite