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18 articles for “Trypsin”
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Research ArticleOpen accessEvery word in the titleThe Use of Artificial Neural Networks in Analysis Cationic Trypsinogen Gene and Hepatitis B Surface AntigenLiu Qicai, Zeng Kai, Zhuang Zehao et al.Problem statement: More and more severe hepatitis cases reported in China are infected with HBV and the immune response of HBV will be reduced if mutations occur in the TCR, therefore it is very important to investigate the relation of T-cellular function and clinical effect by studying the function of T-cell receptor. Approach: Artificial Neural Networks (ANN) was applied to … …American Journal of Immunology, Vol. 5, Issue 2 · 30 Apr 2009 · 7,564 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePurification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity ColumnSanhita Roy, Samir Kumar DuttaProblem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 7,095 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHydroxyl Radical-Scavenging Activity-Guided Extraction of Peptides from Oviductus ranaeJieyang Shen, Yihui Liu, Jiahao Xu et al.Oviductus Ranae (OR) is a traditional Chinese medicinal material that is listed in Chinese Pharmacopeia with proteins the most abundant ingredients. In present work, pepsin/trypsin-based stepwise hydrolysis was applied to extract Antioxidant Peptides from OR (APOR) using hydroxyl radical-scavenging capacity as evaluation index. After single-factor experiment, response surface method coupled with Box-Behnken design were used for optimizing process parameters and … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 18 Oct 2021 · 6,609 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOSNicole Mika, Katharina Cierpka, Michaela C. Lange et al.As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Feb 2014 · 8,157 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDFCite
Research ArticleOpen accessIn the keywordsProtective Effects of Covalent Cross-Linking on Proteolysis of Human Coproporphyrinogen Oxidase and Implications for PorphyriaAhmed W. Jafri, Jason R. Stephenson, Justin B. Morgenthaler et al.The effects of covalent cross-linkers on the enzyme, coproporphyrinogen oxidase, had been previously studied but their role in protecting the enzyme from protease cleavage has not been evaluated. Therefore, we examined how the cross-linker bis (sulfosuccinimidyl) suberate (BS3) affects the ability of trypsin to digest purified, wild type recombinant human coproporphyrinogen oxidase and selected mutants. Following incubation, the apparent molecular … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,687 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffects of Steam Conditioning and Extrusion Temperature on Some Anti-nutritional Factors of Soyabean (Glycine max) for Pet Food ApplicationsB. Purushotham, P.M. Radhakrishna, B.S. SherigaraExtrusion processing of vegetable ingredients such as soyabean (Glycine max) characteristically depends on associating process conditions that influence the product qualities. The process parameters were optimized for extrusion of steam-conditioned material in order to obtain the maximum nutritive value by inactivating the anti-nutritional factors such as urease, trypsin inhibitors and lipase. The processing conditions such as moisture content, temperature and … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Jul 2007 · 9,021 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Feed Additives on the Development of Proteolytic Enzymes of the Tropical Sport Fish Malaysian Mahseer (Tor tambroides-Bleeker) FryK.C.A. Jalal, M. A. Ambak, M.A.B. Abol et al.Tor tambroides fry with a mean of 8.0±0.72 mm standard length (SL) and weighing (W) 0.06±0.01g were stocked at the rate of fifty (50) individuals in each of the fifteen 150l rectangular fibre glass tanks for a period of 5 weeks. The development of proteolytic enzymes (Trypsin and Chymotrypsin) was studied during these feeding treatments. 45% protein diet without additives … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,999 accessesView PDFCite
Research ArticleOpen accessIn the keywordsExtraction, Purification and Characterization of Fish Chymotrypsin: A ReviewLiang Zhou, Suzanne M. Budge, Abdel E. Ghaly et al.Problem statement: Solid fish waste is generated from the unwanted parts of fish including heads, tails, fins, frames, offal (guts, kidney and liver) and skin. It accounts for up to 80% of material from production of surimi, 66% from production of fillet and 27% from production of headed and gutted fish. Currently, fish wastes are disposed off in land-based waste … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 22 Sep 2011 · 10,585 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Sturgeon Protein Fractions on Active Peptide Generation via Enzymatic HydrolysisMengjie Lu, Yingmei Hou, Yejing Sun et al.To identify the sturgeon protein component responsible for generating bioactive peptides, including antioxidant peptides and alcohol dehydrogenase (ADH) promoting peptides, sturgeon protein and its isolated protein components (sarcoplasmic, myofibrillar, and stromal proteins) were studied. Among the four isolated protein components, stromal protein demonstrated the highest susceptibility to enzymatic hydrolysis (P<0.05). Besides, pepsin and alkaline protease are the most effective enzymes … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 1 · 8 Apr 2026 · 1,419 accessesView PDFCite