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20 articles for “Thermophilic”
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Research ArticleOpen accessEvery word in the titleOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,935 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExploration and Characterization of Thermophilic Bacteria from the Kerinci-Jambi Geothermal Source for Alcohol ProductionAnthoni Agustien, Weni Cahyati, Yetria RildaAlcohol produced by thermophilic bacteria is renewable and a safe fuel because it is mainly based on microbial fermentation. This research needs to be done to find out the potential alcohol production of thermophilic bacteria because have many advantages such as degrading a much wider range of carbohydrates, the possibility of direct alcohol recovery from the fermentation broth, tolerance to … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 10 Feb 2024 · 5,199 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEnhanced Amylase Activity by Modulating Abiotic Factors and Enzyme Stability in the Thermophilic Bacterial IsolatesAnthoni Agustien, Denny Bendrianis, Feskaharny Alamsyah et al.Amylase is one of the enzymes that is widely used in bioprocess technology, amylase from different microbial sources also varies in form, so it is suitable for its specific application. The purpose of this study was to determine the optimum temperature, pH, agitation, and stability of the amylase enzyme from two thermophilic bacterial isolates, TUA-07 and TUA-09. This study used … …OnLine Journal of Biological Sciences, Vol. 24, Issue 2 · 7 Feb 2024 · 5,493 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIsolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent CompostAzhari Samsu Baharuddin, Mohamad Nafis Abd Razak, Lim Siong Hock et al.Problems statement: Lack of information on locally isolated cellulase-producing bacterium in thermophilic compost using a mixture of Empty Fruit Bunch (EFB) and Palm Oil Mill Effluent (POME) as composting materials. Approach: The isolation of microbes from compost heap was conducted at day 7 of composting process where the mixture of composting materials consisted of 45.8% cellulose, 17.1% hemicellulose and 28.3% … …American Journal of Applied Sciences, Vol. 7, Issue 1 · 31 Jan 2010 · 12,155 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffective Thermophilic Composting of Crop Residues for Inactivation of Tobacco Mosaic VirusA.E. Ghaly, F. Alkoaik, A. Snow, R. SinghAn effective thermophilic composting bioreactor, in which a homogenous distribution of temperature was maintained at 63-65°C by the addition of a bioavailable carbon and low mixing, was developed. The bioreactor operated on a mixture of tomato plant residues-wood shavings-municipal solid waste compost infected with tobacco mosaic virus (TMV). The initial C: N ratio and moisture content of the compost mixture … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 3 · 30 Sep 2006 · 6,963 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleUse of a Mixture of Thermophilic Enzymes Produced by the Fungus Thermoascus aurantiacus to Enhance the Enzymatic Hydrolysis of the Sugarcane Bagasse CelluloseJ. R. Monte, W. Carvalho, A. M. F. MilagresProblem statement: The production of hydrolytic enzymes by T. aurantiacus has been performed under solid-state fermentations using lignocellulosic materials. The influences of the inoculum size and of the fermentation medium on the production of hemicellulases and cellulases were studied. Filtrates from the cultures were used to hydrolyze a pulp of sugarcane bagasse and the produced enzymes were shown to be … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 13 Dec 2010 · 9,212 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCharacteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71Farouk Al-Quadan, Hazem Akel, Rasha NatshiProblem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,400 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCarbon Dioxide Utilization for an Enhanced Biohydrogen Production of a Biomass HydrolysateAlma Negre Abug, Young Sook OhBio-hydrogen (H2) production in a thermophilic anaerobic bioreactor with carbon dioxide (CO2) utilization at the headspace was investigated at different ratio of Biomass substrate to Microorganism (B/M). A 3 mL volume of 80% NaOH (m/v) solution was continuously hung at the headspace of the bioreactors to capture carbon dioxide. The utilization of CO2 at its headspace, proved to enhance bio … …American Journal of Environmental Sciences, Vol. 12, Issue 4 · 9 Aug 2016 · 7,229 accessesView PDFCite
Research ArticleOpen accessIn the keywordsConformal Mapping of Temperature Variation in Compost HeapJonah Chukwuemeka Agunwamba, Oji Achuka NwokeLaboratory in vessel trapezoidal compost system was designed and constructed. Temperature variation in the compost heap was measured for five days at various depths. Mathematical model for the variation of temperature of the composting process was developed using Conformal Mapping Technique. One of the parameters in the model had two distinct roots, one a positive root and the other negative … …American Journal of Environmental Sciences, Vol. 12, Issue 3 · 17 Jun 2016 · 7,347 accessesView PDFCite
Research ArticleOpen accessIn the keywordsKinetics of Xylanase Fermentation by Recombinant Escherichia coli DH5α in Shake Flask CultureFarliahati Mohd Rusli, Mohd Shamzi Mohamed, Rosfarizan Mohamad et al.Problem statement: Interest in xylanase enzyme application has markedly increased in pulp and paper processing industries. The switch to xylanase-producing recombinant Escherichia coli DH5α pTP510 is seen here as an economic alternative towards higher productivity and easier downstream purification. Modeling of E. coli DH5α growth and enzyme secretion is thus desired for future optimization in fermentation process. Approach: Kinetics of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,480 accessesView PDFCite