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Research ArticleOpen accessEvery word in the titleThe Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in Pichia pastoris KM71Popi Asri Kurniatin, Fifi Fitriyah Masduki, Neni Nurainy et al.A spacer peptide (Glu-Ala repeats) connects N-terminal of a polypeptide precursor to the signal peptide of α-mating factor (α-MF) from Saccharomyces cerevisiae. During the polypeptide precursor secretion, the signal peptide will be first cleaved by signal peptidase in the endoplasmic reticulum, followed by Kex2 endopeptidase and finally the spacer peptide is removed by dipeptidyl aminopeptidase in the Golgi apparatus. In … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 31 Oct 2019 · 9,026 accessesView PDF Cite

1 article for “Spacer Peptide”