Research ArticleOpen accessIn the keywordsBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,854 accessesView PDFCite