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18 articles for “RT-qPCR”
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Case ReportOpen accessPhrase in the titleCOVID-19 Diagnosis in Asymptomatic: RT-qPCR or any other Cost-Effective Method?Mayank Agarwal, Yogesh Arvind Bahurupi, Prasan Kumar PandaOn January 30th, 2020, the World Health Organization announced the COVID-19 outbreak as a Public Health Emergency of International Concern. In the view of this pandemic, early diagnosis is the mainstay for halting the disease progression. Quantitative real-time Reverse Transcriptase-Polymerase Chain Reaction (RT-qPCR) has been established as the cornerstone for the diagnosis of COVID-19. However, the significance of RT-qPCR positivity … …American Journal of Infectious Diseases, Vol. 17, Issue 3 · 2 Jun 2021 · 6,557 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDifferential Gene Transcription in Red Oak (Quercus rubra) Genotypes Resistant to Copper ToxicityProulx Migueal, Paul Michael, Charnelle Djeukam, Kabwe NkongoloToxicity of metals is a major abiotic stressor of plants. Copper (Cu) is one of the most abundant metals in soils from the Greater Sudbury Region (GSR) due to its mining history. Recent studies have described several transporters and chelating proteins involved in copper resistance. Quercus rubra (Red Oak) is a common species that grows in metal contaminated soils in … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 11 Dec 2017 · 8,545 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHigh Level of Nicotianamine Synthase (NAS3) and Natural Resistance Associated Macrophage Protein (NRAMP4) Gene Transcription Induced by Potassium Nitrate in Trembling Aspen (Populus tremuloides)Karolina M. Czajka, Kabwe NkongoloChanges in gene transcription in response to excess metal concentrations have been reported in many organisms, including yeast, microorganisms and plants. Most investigations on the effects of nickel toxicity in plants use commercial salts whose effects have not been analyzed in detail. The main objective of the present study was to determine the effects of different doses of nickel nitrate … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 15 Jul 2018 · 10,884 accessesView PDFCite
Research ArticleOpen accessIn the abstractNitric Oxide Treatment Improves the Antioxidant Capacity While Inhibiting Anthocyanin Biosynthesis of Peach Fruit (‘Xiahui-8’) During RipeningZiqi Wang, Yanyan Li, Yingling Deng et al.Nitric Oxide (NO) is an important bioactive signal molecule that plays an important role in diverse physiological processes. This study aimed to explore the effects of Nitric Oxide (NO) treatments on postharvest peach fruit. Specifically, we examined changes in quality attributes, antioxidant ability, and anthocyanin profiles during storage, given the important role of NO as a bioactive signal molecule in … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 1 · 14 Apr 2026 · 1,392 accessesView PDFCite
Research ArticleOpen accessIn the abstractNerve Growth Factor (NGF) Gene Analysis in Menstrual Blood of Endometriosis: DNA Methylation and mRNA Expression LevelsOcktariyana, Nurul Hikmawati et al.Menstrual blood flow is reversed through the fallopian tubes during the menstrual process and implants outside the uterus, which is the theory of pathogenesis in endometriosis sufferers. This study aims to analyze the level of DNA methylation and mRNA expression of the Nerve Growth Factor (NGF) gene in menstrual blood in endometriosis patients and analyze the correlation. The study was … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 7 Aug 2025 · 4,684 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of Feline Leukemia Virus and Feline Immunodeficiency Virus in Patients from Veterinary Centers in Three Colombian CitiesAna Sofía Mejía-Villada, Maria José Arenas-González, Carolina Ríos Usuga et al.In Colombia, research on Feline Leukemia Virus (FeLV) and Feline Immunodeficiency Virus (FIV) is incipient, despite being the viruses of greatest clinical and epidemiological importance in domestic cats, presenting a worldwide distribution but with very variable prevalence. To determine the molecular prevalence of FeLV and FIV and their associated factors in domestic cats treated in veterinary centers in three Colombian … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 11 Jun 2024 · 7,283 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssociations of HSD17B1 Gene Expression with its DNA Methylation and Estradiol Level in Polycystic Ovary Syndrome Indonesian PatientsRina Puspita, Andon Hestiantoro et al.PCOS's origin and mechanism are still unknown. Epigenetics has been linked to PCOS in an increasing number of studies in recent years. The most extensively researched epigenetic alteration is DNA methylation. During organismal development, DNA methylation can control gene expression by altering transcription factor binding. The alterations in DNA methylation are directly associated with follicular development in PCOS. Studies show … …OnLine Journal of Biological Sciences, Vol. 24, Issue 2 · 8 Jan 2024 · 5,399 accessesView PDFCite
Research ArticleOpen accessIn the abstractDifferential Gene Expression of Peroxide Resistant Protein (AhpC) in Planktonic and Biofilm State of Uropathogenic Escherichia coli CellsOmar Sadik Shalal, Ani-Simona SevastreThe gastrointestinal tract seems to be the primary reservoir of Uropathogenic Escherichia coli (UPEC) in humans. UPEC strains harbor the Urinary Tract (UT) and cause Urinary Tract Infections (UTI) which might represent a serious threat to human life. To counteract with the damage caused by the Reactive Oxygen Species (ROS), bacterial strains produce various enzymes and proteins like Alkyl hydroperoxide … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 3 · 13 Oct 2023 · 5,324 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression of Exosome-Derived MicroRNAs miR-21 and miR-135 are Differentially Regulated Among Dental Pulp Stem CellsMatthew Hunsaker, Brandon Richards, Adelle Fuller et al.Dental Pulp Stem Cells (DPSCs) are a type of mesenchymal stem cell that has the potential to differentiate into various types of cells and tissue. Previous research has demonstrated that DPSCs can be easily accessed and isolated from both permanent and deciduous teeth, such as wisdom teeth or third molars, although there is less information available about the mechanisms and … …Current Research in Medicine, Vol. 11, Issue 1 · 10 Jan 2023 · 10,533 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression of Metallothionein-Like Protein 2B Gene Induced by Different Concentrations of Copper in White Spruce (Picea glauca)Megan McKergow, Kabwe NkongoloThe alterations to the expressions of metal transporter genes can induce metal ion uptake to maintain cell homeostasis when metal concentrations are low. Metallothioneins (MTs) are a class of low molecular mass (4-8 kDa), cysteine (Cys)-rich proteins that can bind metals via the thiol groups of their Cys residues. Studies on MT gene expressions in higher plants, such as conifers, … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 25 Dec 2022 · 5,118 accessesView PDFCite