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6 articles for “Primer Design”
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Research ArticleOpen accessIn the keywordsMolecular Identification of Domestic and Wild Boars Using Cytochrome-b Specific Primers for Halal AuthenticationRifqi, Edi Suryanto, Ari Surya Sukarno et al.The subject of adulterating wild boar and pig meat products with other animal meat is delicate, particularly in the Muslim nation of Indonesia. In recent years, there has been a lot of concern over halal meat products that contain wild boar and pig meat due to Economically Motivated Adulteration (EMA). However, there are limited studies of PCR-DNA-based to discriminate between … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 3 · 14 Nov 2025 · 2,378 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDesign and Evaluation of Three Pair Primers for Exon 1 Amplification of Hyaluroglucosaminidase-1 GeneUsman Sumo Friend Tambunan, Sylvia Sugito, Arli Aditya ParikesitProblem statement: Hyaluronidase is an enzyme which catalyze hydrolysis of Hyaluronan (HA). Hyaluronan is important in cell migration during embryonic development, cellular proliferation and differentiation and has a structural role in connective tissues. Hyaluronidase deficiency is correlated with mucopolysaccharidosis IX. In human, hyaluronidase is encoded by HYAL1 gene. The mutation study of HYAL1 gene was carried out by many researchers, … …OnLine Journal of Biological Sciences, Vol. 10, Issue 2 · 16 Jun 2010 · 8,702 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Forensic Profiling of Primer-Derived Inorganic Gunshot Residues Using SEM-EDX SpectroscopyAhmed M. Bendary Salem, Aldo Barbaro, Bolaji OladipoGunshot Residue (GSR) analysis presents a significant challenge in forensic science due to the need to detect and identify minute traces of powders and initiating compositions formed during the chemical decomposition and deflagration processes within cartridge cases. This research is a qualitative comparative profiling study, designed to create a reference database of the most characteristic elemental markers for commonly used … …American Journal of Applied Sciences, Vol. 22, Issue 1 · 6 Mar 2026 · 2,726 accessesView PDFCite
Research ArticleOpen accessIn the abstractEstablishment and Application of Rapid Quantitative Detection Technology for Bacillus cereusFeilong Sun, Hanyu Li, Jingxue Qian et al.Infections caused by Bacillus cereus often occur, causing a certain degree of health hazards and economic losses to people around the world. Rapid quantitative detection is of great significance for prevention. This study aims to develop a simple and rapid quantitative detection method for B. cereus, which can be used to monitor the contamination of B. cereus in food samples. … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 21 Mar 2023 · 5,564 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment, Polymorphism and Cross-Species Transferability of Genomic SSR Markers in Duabanga Moluccana, an Indigenous Tree Species from SarawakKit-Siong Liew, Wei-Seng Ho, Shek-Ling Pang, Julaihi AbdullahIn this study, we used ISSR-suppression methods to develop a set of SSR markers for Duabanga moluccana. It is an indigenous fast growing tropical tree species. A total of 44 SSR regions were identified and specific primer pairs were designed. The SSR motifs contained perfect compound with 24 (54.5%) occurrences, followed by the imperfect compounds with 8 (18.2%), simple perfect … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 26 Jan 2016 · 7,784 accessesView PDFCite
Research ArticleOpen accessIn the abstractFire Design: Direct Comparison Between Fire Curves. The Case Study of a NurseryMara Lombardi, Giuliano Rossi, Nicolò Sciarretta, Nino OrangesAccording to fire safety engineering, the present study analyzes fire design settings for simulation of fire in a nursery and proposes to compare simulations developed with a natural fire curve and nominal fire curve. Comparative analysis was developed according to thermo-fluid dynamic parameters that are relevant to the safety of the exposed and for the representative period of the danger … …American Journal of Engineering and Applied Sciences, Vol. 6, Issue 3 · 9 Sep 2013 · 7,345 accessesView PDFCite